Capybara microvirus Cap1_SP_161
Average proteome isoelectric point is 5.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4G3|A0A4P8W4G3_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_161 OX=2585397 PE=4 SV=1
MM1 pKa = 7.31 NLYY4 pKa = 10.34 VLYY7 pKa = 10.45 DD8 pKa = 3.69 KK9 pKa = 10.37 EE10 pKa = 4.1 AHH12 pKa = 6.41 IVQCPYY18 pKa = 10.24 PQPLEE23 pKa = 3.84 NDD25 pKa = 4.03 TIARR29 pKa = 11.84 RR30 pKa = 11.84 WWKK33 pKa = 10.46 KK34 pKa = 6.75 STEE37 pKa = 4.13 SLVDD41 pKa = 3.17 VMGILPSMISLYY53 pKa = 10.91 CVGIWNKK60 pKa = 7.95 EE61 pKa = 4.02 TGEE64 pKa = 3.87 ITPCFDD70 pKa = 3.09 EE71 pKa = 4.49 VAVISSDD78 pKa = 3.73 DD79 pKa = 3.28
Molecular weight: 9.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.105
IPC2_protein 4.457
IPC_protein 4.304
Toseland 4.126
ProMoST 4.304
Dawson 4.266
Bjellqvist 4.482
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.202
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.164
EMBOSS 4.177
Sillero 4.418
Patrickios 1.99
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.353
Protein with the highest isoelectric point:
>tr|A0A4P8W5A4|A0A4P8W5A4_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_161 OX=2585397 PE=3 SV=1
MM1 pKa = 7.36 ICSSPKK7 pKa = 10.27 LVYY10 pKa = 10.59 LKK12 pKa = 10.07 NNCSIRR18 pKa = 11.84 VPCGHH23 pKa = 7.46 CINCRR28 pKa = 11.84 IQKK31 pKa = 10.05 SSDD34 pKa = 2.37 WATRR38 pKa = 11.84 ICLEE42 pKa = 4.61 LPFWDD47 pKa = 3.87 SNIFTTLTYY56 pKa = 10.98 DD57 pKa = 4.2 DD58 pKa = 4.92 DD59 pKa = 5.97 HH60 pKa = 8.43 IPSDD64 pKa = 3.59 WSIHH68 pKa = 6.67 KK69 pKa = 10.47 DD70 pKa = 3.26 DD71 pKa = 4.14 LQKK74 pKa = 10.67 FFKK77 pKa = 10.37 RR78 pKa = 11.84 LRR80 pKa = 11.84 RR81 pKa = 11.84 EE82 pKa = 3.26 IEE84 pKa = 3.99 YY85 pKa = 10.11 NAKK88 pKa = 10.61 SNGLEE93 pKa = 4.12 VPKK96 pKa = 10.26 IAYY99 pKa = 7.62 FASGEE104 pKa = 4.14 YY105 pKa = 10.02 GDD107 pKa = 4.4 KK108 pKa = 10.87 SMRR111 pKa = 11.84 PHH113 pKa = 4.89 YY114 pKa = 9.89 HH115 pKa = 6.84 AIIFGVGLDD124 pKa = 3.56 LNNYY128 pKa = 9.04 NNEE131 pKa = 4.01 LQCYY135 pKa = 9.83 YY136 pKa = 10.7 SDD138 pKa = 4.68 CKK140 pKa = 10.87 LIEE143 pKa = 4.46 NNWSYY148 pKa = 11.71 GNVYY152 pKa = 9.76 NGCVSYY158 pKa = 11.29 DD159 pKa = 3.49 SARR162 pKa = 11.84 YY163 pKa = 6.4 TADD166 pKa = 3.33 YY167 pKa = 11.03 VLKK170 pKa = 10.46 QYY172 pKa = 10.94 SGEE175 pKa = 4.11 LKK177 pKa = 10.49 QKK179 pKa = 10.5 VYY181 pKa = 10.46 TDD183 pKa = 3.75 NGLEE187 pKa = 4.2 TPFCIHH193 pKa = 6.82 SNGIGKK199 pKa = 9.68 RR200 pKa = 11.84 FVEE203 pKa = 5.05 VYY205 pKa = 10.05 EE206 pKa = 4.1 SDD208 pKa = 3.77 IIGKK212 pKa = 9.89 GYY214 pKa = 10.22 IIIRR218 pKa = 11.84 GVKK221 pKa = 9.38 KK222 pKa = 10.15 ALPRR226 pKa = 11.84 YY227 pKa = 9.64 FYY229 pKa = 10.73 DD230 pKa = 3.58 ILNKK234 pKa = 9.81 KK235 pKa = 8.21 YY236 pKa = 9.62 RR237 pKa = 11.84 WHH239 pKa = 6.01 KK240 pKa = 10.77 CEE242 pKa = 4.08 YY243 pKa = 10.71 DD244 pKa = 3.87 DD245 pKa = 5.23 DD246 pKa = 5.77 GKK248 pKa = 10.92 MIFGFSYY255 pKa = 10.6 FEE257 pKa = 3.97 NFQKK261 pKa = 10.65 HH262 pKa = 3.91 MKK264 pKa = 9.37 NYY266 pKa = 9.31 KK267 pKa = 10.24 GEE269 pKa = 4.06 YY270 pKa = 10.05 LEE272 pKa = 4.53 ADD274 pKa = 3.1 QMEE277 pKa = 5.06 LFRR280 pKa = 11.84 RR281 pKa = 11.84 WRR283 pKa = 11.84 DD284 pKa = 3.3 EE285 pKa = 3.92 CKK287 pKa = 10.29 EE288 pKa = 4.52 LYY290 pKa = 10.63 DD291 pKa = 4.86 CDD293 pKa = 4.2 VSFDD297 pKa = 4.9 DD298 pKa = 5.49 FLQKK302 pKa = 10.38 EE303 pKa = 4.25 KK304 pKa = 11.09 CDD306 pKa = 4.13 FSDD309 pKa = 5.11 KK310 pKa = 10.67 IEE312 pKa = 4.17 QNVARR317 pKa = 11.84 NKK319 pKa = 10.46 LNEE322 pKa = 3.94 HH323 pKa = 6.54 KK324 pKa = 10.63 KK325 pKa = 10.62 IIDD328 pKa = 3.6 AQRR331 pKa = 11.84 SLII334 pKa = 3.94
Molecular weight: 39.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.097
IPC2_protein 6.262
IPC_protein 6.338
Toseland 6.376
ProMoST 6.781
Dawson 6.766
Bjellqvist 6.751
Wikipedia 6.751
Rodwell 6.751
Grimsley 6.453
Solomon 6.766
Lehninger 6.766
Nozaki 7.059
DTASelect 7.132
Thurlkill 7.161
EMBOSS 7.146
Sillero 7.176
Patrickios 3.541
IPC_peptide 6.781
IPC2_peptide 6.854
IPC2.peptide.svr19 6.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1425
79
558
285.0
32.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.053 ± 0.72
1.825 ± 0.627
7.509 ± 0.825
5.614 ± 0.556
4.912 ± 0.695
5.895 ± 0.531
1.684 ± 0.398
5.263 ± 0.73
6.877 ± 1.527
8.07 ± 0.531
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.456 ± 0.278
5.895 ± 0.498
3.368 ± 0.955
5.053 ± 1.018
4.07 ± 0.409
9.404 ± 0.718
4.14 ± 0.822
5.965 ± 0.632
1.544 ± 0.307
5.404 ± 0.831
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here