Firmicutes bacterium CAG:631

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1348 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5VX83|R5VX83_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:631 OX=1262996 GN=BN742_00171 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.33KK3 pKa = 9.61IFKK6 pKa = 10.64SFFLMMMIVFSAFSMTGCTGSFFGSDD32 pKa = 3.06EE33 pKa = 3.78QGIQIEE39 pKa = 4.93SITSEE44 pKa = 4.07EE45 pKa = 4.47LEE47 pKa = 4.8DD48 pKa = 4.29GSMLITITYY57 pKa = 8.32TDD59 pKa = 4.16EE60 pKa = 4.64DD61 pKa = 4.18RR62 pKa = 11.84EE63 pKa = 4.38PTTFLIPKK71 pKa = 9.85AEE73 pKa = 4.57DD74 pKa = 3.63GEE76 pKa = 4.39PGKK79 pKa = 10.4DD80 pKa = 3.18GNGIQGITYY89 pKa = 8.04TLSEE93 pKa = 5.36DD94 pKa = 3.71GLSTIVTITYY104 pKa = 8.2TDD106 pKa = 3.37QSIEE110 pKa = 4.06PTQITIPNGISVTGVNYY127 pKa = 10.09VVDD130 pKa = 4.16EE131 pKa = 4.39VTGNTLITLQYY142 pKa = 11.09SDD144 pKa = 4.51GTTSDD149 pKa = 5.32PIPVPKK155 pKa = 10.56GEE157 pKa = 4.03QGEE160 pKa = 4.2PGEE163 pKa = 5.14DD164 pKa = 3.43GNSIVGIDD172 pKa = 4.4QIVNDD177 pKa = 5.03DD178 pKa = 4.44DD179 pKa = 4.64SVTLIFHH186 pKa = 6.66FSKK189 pKa = 10.95SEE191 pKa = 3.98DD192 pKa = 3.76YY193 pKa = 10.51IVQIPAPHH201 pKa = 7.1EE202 pKa = 4.04GRR204 pKa = 11.84GIDD207 pKa = 3.63TIVAGEE213 pKa = 4.26TNDD216 pKa = 3.39QYY218 pKa = 11.91TMTIYY223 pKa = 10.97YY224 pKa = 10.29SDD226 pKa = 3.83GTDD229 pKa = 3.73EE230 pKa = 4.2TLTWDD235 pKa = 4.77KK236 pKa = 11.05PNPNQWYY243 pKa = 8.18TGSEE247 pKa = 4.06PSNSLGVDD255 pKa = 2.87GDD257 pKa = 4.18FYY259 pKa = 11.68FDD261 pKa = 3.79IYY263 pKa = 10.43HH264 pKa = 6.37HH265 pKa = 6.71NIYY268 pKa = 10.3VKK270 pKa = 10.65QSGVWVLIVSFDD282 pKa = 3.95NNIEE286 pKa = 4.04THH288 pKa = 6.18SVIFQLNDD296 pKa = 3.52SLDD299 pKa = 3.8EE300 pKa = 4.24PANMPSGSLMAYY312 pKa = 8.7QIPHH316 pKa = 6.48GSYY319 pKa = 9.48FAAQEE324 pKa = 4.99GYY326 pKa = 8.89TIPIPTRR333 pKa = 11.84NGYY336 pKa = 10.32DD337 pKa = 3.27FVGWYY342 pKa = 5.06TTKK345 pKa = 10.61TITPTSGKK353 pKa = 8.66FTDD356 pKa = 4.56LTPVMADD363 pKa = 3.15LTLYY367 pKa = 10.42AIWEE371 pKa = 4.31KK372 pKa = 10.77QVV374 pKa = 2.84

Molecular weight: 41.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5VQC5|R5VQC5_9FIRM FtsK/SpoIIIE family DNA translocase OS=Firmicutes bacterium CAG:631 OX=1262996 GN=BN742_00778 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.6RR10 pKa = 11.84KK11 pKa = 9.23HH12 pKa = 6.11KK13 pKa = 10.02NVHH16 pKa = 4.84GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPNGRR28 pKa = 11.84KK29 pKa = 9.3VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84NRR39 pKa = 11.84QVLSAA44 pKa = 4.43

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1348

0

1348

426106

30

2699

316.1

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.492 ± 0.059

1.309 ± 0.028

5.427 ± 0.052

6.737 ± 0.07

4.955 ± 0.059

5.334 ± 0.058

2.014 ± 0.032

9.101 ± 0.075

8.395 ± 0.085

9.734 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.03

5.761 ± 0.061

2.934 ± 0.036

4.298 ± 0.054

2.913 ± 0.041

6.081 ± 0.065

5.405 ± 0.073

6.061 ± 0.059

0.712 ± 0.019

4.943 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski