Amino acid dipepetide frequency for Firmicutes bacterium CAG:631

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.034AlaAla: 3.034 ± 0.122	0.798AlaCys: 0.798 ± 0.045	2.481AlaAsp: 2.481 ± 0.091	2.445AlaGlu: 2.445 ± 0.094	2.915AlaPhe: 2.915 ± 0.127	2.802AlaGly: 2.802 ± 0.093	1.049AlaHis: 1.049 ± 0.05	5.708AlaIle: 5.708 ± 0.115	4.903AlaLys: 4.903 ± 0.113	6.17AlaLeu: 6.17 ± 0.157	1.46AlaMet: 1.46 ± 0.059	2.962AlaAsn: 2.962 ± 0.094	1.42AlaPro: 1.42 ± 0.064	2.093AlaGln: 2.093 ± 0.077	1.629AlaArg: 1.629 ± 0.066	3.581AlaSer: 3.581 ± 0.097	3.49AlaThr: 3.49 ± 0.104	2.95AlaVal: 2.95 ± 0.087	0.357AlaTrp: 0.357 ± 0.025	2.675AlaTyr: 2.675 ± 0.086	0.0AlaXaa: 0.0 ± 0.0
Cys	0.659CysAla: 0.659 ± 0.039	0.239CysCys: 0.239 ± 0.036	0.777CysAsp: 0.777 ± 0.041	0.695CysGlu: 0.695 ± 0.043	0.77CysPhe: 0.77 ± 0.044	0.929CysGly: 0.929 ± 0.056	0.38CysHis: 0.38 ± 0.032	1.15CysIle: 1.15 ± 0.057	0.983CysLys: 0.983 ± 0.051	1.321CysLeu: 1.321 ± 0.044	0.23CysMet: 0.23 ± 0.026	0.622CysAsn: 0.622 ± 0.039	0.552CysPro: 0.552 ± 0.045	0.568CysGln: 0.568 ± 0.043	0.394CysArg: 0.394 ± 0.033	0.843CysSer: 0.843 ± 0.044	0.537CysThr: 0.537 ± 0.039	0.8CysVal: 0.8 ± 0.053	0.075CysTrp: 0.075 ± 0.016	0.568CysTyr: 0.568 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	3.325AspAla: 3.325 ± 0.082	0.674AspCys: 0.674 ± 0.037	2.88AspAsp: 2.88 ± 0.099	4.673AspGlu: 4.673 ± 0.107	3.232AspPhe: 3.232 ± 0.093	3.243AspGly: 3.243 ± 0.125	1.051AspHis: 1.051 ± 0.054	4.656AspIle: 4.656 ± 0.109	3.6AspLys: 3.6 ± 0.111	5.611AspLeu: 5.611 ± 0.113	1.115AspMet: 1.115 ± 0.055	2.283AspAsn: 2.283 ± 0.083	1.551AspPro: 1.551 ± 0.059	2.133AspGln: 2.133 ± 0.068	1.429AspArg: 1.429 ± 0.067	3.02AspSer: 3.02 ± 0.101	2.535AspThr: 2.535 ± 0.08	3.527AspVal: 3.527 ± 0.095	0.397AspTrp: 0.397 ± 0.031	3.337AspTyr: 3.337 ± 0.098	0.0AspXaa: 0.0 ± 0.0
Glu	3.558GluAla: 3.558 ± 0.081	0.706GluCys: 0.706 ± 0.038	3.71GluAsp: 3.71 ± 0.111	5.461GluGlu: 5.461 ± 0.147	2.701GluPhe: 2.701 ± 0.085	3.182GluGly: 3.182 ± 0.099	0.976GluHis: 0.976 ± 0.061	6.332GluIle: 6.332 ± 0.118	7.156GluLys: 7.156 ± 0.142	5.78GluLeu: 5.78 ± 0.123	2.007GluMet: 2.007 ± 0.074	5.424GluAsn: 5.424 ± 0.135	1.413GluPro: 1.413 ± 0.061	2.513GluGln: 2.513 ± 0.085	1.913GluArg: 1.913 ± 0.076	3.154GluSer: 3.154 ± 0.1	3.579GluThr: 3.579 ± 0.105	4.335GluVal: 4.335 ± 0.097	0.49GluTrp: 0.49 ± 0.032	2.976GluTyr: 2.976 ± 0.084	0.005GluXaa: 0.005 ± 0.003
Phe	2.781PheAla: 2.781 ± 0.103	0.652PheCys: 0.652 ± 0.043	3.164PheAsp: 3.164 ± 0.083	3.175PheGlu: 3.175 ± 0.085	2.542PhePhe: 2.542 ± 0.091	2.499PheGly: 2.499 ± 0.094	0.932PheHis: 0.932 ± 0.056	4.776PheIle: 4.776 ± 0.134	3.647PheLys: 3.647 ± 0.104	5.097PheLeu: 5.097 ± 0.14	1.131PheMet: 1.131 ± 0.043	2.729PheAsn: 2.729 ± 0.09	1.342PhePro: 1.342 ± 0.061	1.683PheGln: 1.683 ± 0.08	1.126PheArg: 1.126 ± 0.047	3.649PheSer: 3.649 ± 0.091	2.445PheThr: 2.445 ± 0.085	3.241PheVal: 3.241 ± 0.104	0.317PheTrp: 0.317 ± 0.031	2.619PheTyr: 2.619 ± 0.085	0.0PheXaa: 0.0 ± 0.0
Gly	2.99GlyAla: 2.99 ± 0.102	0.932GlyCys: 0.932 ± 0.049	2.513GlyAsp: 2.513 ± 0.086	3.119GlyGlu: 3.119 ± 0.088	2.551GlyPhe: 2.551 ± 0.09	3.274GlyGly: 3.274 ± 0.105	1.136GlyHis: 1.136 ± 0.055	5.175GlyIle: 5.175 ± 0.121	4.684GlyLys: 4.684 ± 0.099	4.597GlyLeu: 4.597 ± 0.118	1.432GlyMet: 1.432 ± 0.062	2.924GlyAsn: 2.924 ± 0.092	1.028GlyPro: 1.028 ± 0.055	1.596GlyGln: 1.596 ± 0.062	1.711GlyArg: 1.711 ± 0.085	3.25GlySer: 3.25 ± 0.108	3.328GlyThr: 3.328 ± 0.12	3.577GlyVal: 3.577 ± 0.113	0.493GlyTrp: 0.493 ± 0.038	3.027GlyTyr: 3.027 ± 0.099	0.002GlyXaa: 0.002 ± 0.002
His	1.042HisAla: 1.042 ± 0.052	0.333HisCys: 0.333 ± 0.027	1.007HisAsp: 1.007 ± 0.055	1.166HisGlu: 1.166 ± 0.056	1.129HisPhe: 1.129 ± 0.059	1.056HisGly: 1.056 ± 0.055	0.669HisHis: 0.669 ± 0.048	1.601HisIle: 1.601 ± 0.066	1.054HisLys: 1.054 ± 0.049	2.546HisLeu: 2.546 ± 0.093	0.406HisMet: 0.406 ± 0.033	0.732HisAsn: 0.732 ± 0.041	0.962HisPro: 0.962 ± 0.052	1.366HisGln: 1.366 ± 0.07	0.667HisArg: 0.667 ± 0.042	1.164HisSer: 1.164 ± 0.044	0.873HisThr: 0.873 ± 0.04	1.204HisVal: 1.204 ± 0.057	0.122HisTrp: 0.122 ± 0.018	1.044HisTyr: 1.044 ± 0.05	0.0HisXaa: 0.0 ± 0.0
Ile	5.649IleAla: 5.649 ± 0.109	1.31IleCys: 1.31 ± 0.051	5.576IleAsp: 5.576 ± 0.109	6.29IleGlu: 6.29 ± 0.123	4.248IlePhe: 4.248 ± 0.129	4.957IleGly: 4.957 ± 0.11	2.103IleHis: 2.103 ± 0.077	7.562IleIle: 7.562 ± 0.193	6.475IleLys: 6.475 ± 0.141	9.301IleLeu: 9.301 ± 0.209	1.762IleMet: 1.762 ± 0.072	4.33IleAsn: 4.33 ± 0.096	3.215IlePro: 3.215 ± 0.111	4.778IleGln: 4.778 ± 0.135	2.701IleArg: 2.701 ± 0.085	5.398IleSer: 5.398 ± 0.138	4.727IleThr: 4.727 ± 0.126	5.973IleVal: 5.973 ± 0.11	0.575IleTrp: 0.575 ± 0.037	4.081IleTyr: 4.081 ± 0.11	0.002IleXaa: 0.002 ± 0.002
Lys	4.121LysAla: 4.121 ± 0.097	0.753LysCys: 0.753 ± 0.045	5.24LysAsp: 5.24 ± 0.116	8.749LysGlu: 8.749 ± 0.168	2.267LysPhe: 2.267 ± 0.072	3.922LysGly: 3.922 ± 0.104	1.333LysHis: 1.333 ± 0.064	7.787LysIle: 7.787 ± 0.145	9.732LysLys: 9.732 ± 0.183	5.712LysLeu: 5.712 ± 0.12	2.638LysMet: 2.638 ± 0.086	6.052LysAsn: 6.052 ± 0.13	1.887LysPro: 1.887 ± 0.063	3.382LysGln: 3.382 ± 0.112	2.837LysArg: 2.837 ± 0.084	3.753LysSer: 3.753 ± 0.113	4.56LysThr: 4.56 ± 0.097	5.02LysVal: 5.02 ± 0.111	0.589LysTrp: 0.589 ± 0.042	3.612LysTyr: 3.612 ± 0.1	0.0LysXaa: 0.0 ± 0.0
Leu	5.346LeuAla: 5.346 ± 0.122	1.312LeuCys: 1.312 ± 0.053	5.109LeuAsp: 5.109 ± 0.131	5.846LeuGlu: 5.846 ± 0.135	5.21LeuPhe: 5.21 ± 0.124	4.769LeuGly: 4.769 ± 0.122	1.746LeuHis: 1.746 ± 0.073	8.068LeuIle: 8.068 ± 0.165	9.756LeuLys: 9.756 ± 0.197	9.521LeuLeu: 9.521 ± 0.199	2.248LeuMet: 2.248 ± 0.079	5.912LeuAsn: 5.912 ± 0.132	3.213LeuPro: 3.213 ± 0.082	3.835LeuGln: 3.835 ± 0.113	2.49LeuArg: 2.49 ± 0.075	7.282LeuSer: 7.282 ± 0.144	5.621LeuThr: 5.621 ± 0.136	5.358LeuVal: 5.358 ± 0.095	0.62LeuTrp: 0.62 ± 0.035	4.074LeuTyr: 4.074 ± 0.105	0.007LeuXaa: 0.007 ± 0.004
Met	1.488MetAla: 1.488 ± 0.065	0.244MetCys: 0.244 ± 0.024	1.537MetAsp: 1.537 ± 0.068	1.537MetGlu: 1.537 ± 0.066	0.899MetPhe: 0.899 ± 0.055	1.345MetGly: 1.345 ± 0.061	0.415MetHis: 0.415 ± 0.03	2.511MetIle: 2.511 ± 0.084	2.276MetLys: 2.276 ± 0.069	1.95MetLeu: 1.95 ± 0.068	0.73MetMet: 0.73 ± 0.043	1.68MetAsn: 1.68 ± 0.061	0.735MetPro: 0.735 ± 0.039	1.016MetGln: 1.016 ± 0.055	0.617MetArg: 0.617 ± 0.037	1.249MetSer: 1.249 ± 0.06	1.272MetThr: 1.272 ± 0.057	1.495MetVal: 1.495 ± 0.059	0.129MetTrp: 0.129 ± 0.017	0.735MetTyr: 0.735 ± 0.039	0.0MetXaa: 0.0 ± 0.0
Asn	3.22AsnAla: 3.22 ± 0.094	0.732AsnCys: 0.732 ± 0.047	3.145AsnAsp: 3.145 ± 0.098	4.398AsnGlu: 4.398 ± 0.113	2.546AsnPhe: 2.546 ± 0.075	3.403AsnGly: 3.403 ± 0.101	1.497AsnHis: 1.497 ± 0.066	4.647AsnIle: 4.647 ± 0.105	4.236AsnLys: 4.236 ± 0.124	5.125AsnLeu: 5.125 ± 0.116	1.213AsnMet: 1.213 ± 0.053	2.992AsnAsn: 2.992 ± 0.095	2.185AsnPro: 2.185 ± 0.078	3.903AsnGln: 3.903 ± 0.118	1.873AsnArg: 1.873 ± 0.062	2.858AsnSer: 2.858 ± 0.101	2.729AsnThr: 2.729 ± 0.086	3.358AsnVal: 3.358 ± 0.079	0.408AsnTrp: 0.408 ± 0.03	3.142AsnTyr: 3.142 ± 0.093	0.002AsnXaa: 0.002 ± 0.002
Pro	1.366ProAla: 1.366 ± 0.068	0.35ProCys: 0.35 ± 0.033	1.354ProAsp: 1.354 ± 0.06	1.72ProGlu: 1.72 ± 0.065	1.906ProPhe: 1.906 ± 0.073	1.368ProGly: 1.368 ± 0.067	0.697ProHis: 0.697 ± 0.038	2.931ProIle: 2.931 ± 0.079	1.927ProLys: 1.927 ± 0.069	3.138ProLeu: 3.138 ± 0.09	0.643ProMet: 0.643 ± 0.04	1.748ProAsn: 1.748 ± 0.07	0.535ProPro: 0.535 ± 0.037	1.183ProGln: 1.183 ± 0.044	0.685ProArg: 0.685 ± 0.039	2.187ProSer: 2.187 ± 0.078	1.849ProThr: 1.849 ± 0.067	1.835ProVal: 1.835 ± 0.067	0.23ProTrp: 0.23 ± 0.027	1.69ProTyr: 1.69 ± 0.071	0.0ProXaa: 0.0 ± 0.0
Gln	2.26GlnAla: 2.26 ± 0.084	0.46GlnCys: 0.46 ± 0.033	2.129GlnAsp: 2.129 ± 0.073	3.288GlnGlu: 3.288 ± 0.103	1.784GlnPhe: 1.784 ± 0.067	1.734GlnGly: 1.734 ± 0.06	0.725GlnHis: 0.725 ± 0.041	3.929GlnIle: 3.929 ± 0.101	4.494GlnLys: 4.494 ± 0.125	4.231GlnLeu: 4.231 ± 0.126	1.19GlnMet: 1.19 ± 0.052	2.812GlnAsn: 2.812 ± 0.097	1.094GlnPro: 1.094 ± 0.046	1.927GlnGln: 1.927 ± 0.08	1.265GlnArg: 1.265 ± 0.06	2.389GlnSer: 2.389 ± 0.079	2.309GlnThr: 2.309 ± 0.079	2.323GlnVal: 2.323 ± 0.088	0.422GlnTrp: 0.422 ± 0.034	2.215GlnTyr: 2.215 ± 0.09	0.002GlnXaa: 0.002 ± 0.003
Arg	1.45ArgAla: 1.45 ± 0.062	0.507ArgCys: 0.507 ± 0.038	1.286ArgAsp: 1.286 ± 0.056	1.586ArgGlu: 1.586 ± 0.065	1.612ArgPhe: 1.612 ± 0.068	1.486ArgGly: 1.486 ± 0.068	0.655ArgHis: 0.655 ± 0.043	2.795ArgIle: 2.795 ± 0.091	2.485ArgLys: 2.485 ± 0.086	2.997ArgLeu: 2.997 ± 0.085	0.735ArgMet: 0.735 ± 0.042	1.723ArgAsn: 1.723 ± 0.067	0.911ArgPro: 0.911 ± 0.049	1.131ArgGln: 1.131 ± 0.056	1.136ArgArg: 1.136 ± 0.056	1.46ArgSer: 1.46 ± 0.07	1.389ArgThr: 1.389 ± 0.055	1.922ArgVal: 1.922 ± 0.064	0.23ArgTrp: 0.23 ± 0.022	1.636ArgTyr: 1.636 ± 0.06	0.0ArgXaa: 0.0 ± 0.0
Ser	2.865SerAla: 2.865 ± 0.098	0.873SerCys: 0.873 ± 0.046	2.83SerAsp: 2.83 ± 0.098	3.009SerGlu: 3.009 ± 0.088	4.006SerPhe: 4.006 ± 0.108	3.485SerGly: 3.485 ± 0.098	1.037SerHis: 1.037 ± 0.049	5.895SerIle: 5.895 ± 0.129	4.614SerLys: 4.614 ± 0.097	6.738SerLeu: 6.738 ± 0.147	1.469SerMet: 1.469 ± 0.056	3.64SerAsn: 3.64 ± 0.113	1.511SerPro: 1.511 ± 0.06	2.157SerGln: 2.157 ± 0.071	1.68SerArg: 1.68 ± 0.059	4.715SerSer: 4.715 ± 0.147	3.267SerThr: 3.267 ± 0.101	3.394SerVal: 3.394 ± 0.106	0.422SerTrp: 0.422 ± 0.035	3.194SerTyr: 3.194 ± 0.095	0.005SerXaa: 0.005 ± 0.003
Thr	2.87ThrAla: 2.87 ± 0.098	0.554ThrCys: 0.554 ± 0.035	2.436ThrAsp: 2.436 ± 0.078	2.373ThrGlu: 2.373 ± 0.09	3.103ThrPhe: 3.103 ± 0.109	3.117ThrGly: 3.117 ± 0.112	1.08ThrHis: 1.08 ± 0.052	5.963ThrIle: 5.963 ± 0.144	4.194ThrLys: 4.194 ± 0.096	5.691ThrLeu: 5.691 ± 0.135	1.138ThrMet: 1.138 ± 0.047	3.189ThrAsn: 3.189 ± 0.095	2.068ThrPro: 2.068 ± 0.08	1.913ThrGln: 1.913 ± 0.062	1.439ThrArg: 1.439 ± 0.057	3.574ThrSer: 3.574 ± 0.104	3.281ThrThr: 3.281 ± 0.123	2.708ThrVal: 2.708 ± 0.106	0.441ThrTrp: 0.441 ± 0.036	2.924ThrTyr: 2.924 ± 0.117	0.0ThrXaa: 0.0 ± 0.0
Val	3.75ValAla: 3.75 ± 0.116	0.894ValCys: 0.894 ± 0.054	3.544ValAsp: 3.544 ± 0.101	4.152ValGlu: 4.152 ± 0.113	3.044ValPhe: 3.044 ± 0.078	3.593ValGly: 3.593 ± 0.098	1.096ValHis: 1.096 ± 0.052	5.142ValIle: 5.142 ± 0.112	4.229ValLys: 4.229 ± 0.115	6.099ValLeu: 6.099 ± 0.128	1.302ValMet: 1.302 ± 0.052	3.023ValAsn: 3.023 ± 0.1	1.781ValPro: 1.781 ± 0.069	2.328ValGln: 2.328 ± 0.081	1.701ValArg: 1.701 ± 0.076	4.093ValSer: 4.093 ± 0.091	3.34ValThr: 3.34 ± 0.127	4.255ValVal: 4.255 ± 0.127	0.397ValTrp: 0.397 ± 0.032	2.847ValTyr: 2.847 ± 0.083	0.0ValXaa: 0.0 ± 0.0
Trp	0.354TrpAla: 0.354 ± 0.032	0.075TrpCys: 0.075 ± 0.014	0.364TrpAsp: 0.364 ± 0.031	0.422TrpGlu: 0.422 ± 0.031	0.404TrpPhe: 0.404 ± 0.032	0.401TrpGly: 0.401 ± 0.033	0.174TrpHis: 0.174 ± 0.02	0.699TrpIle: 0.699 ± 0.04	0.505TrpLys: 0.505 ± 0.036	0.749TrpLeu: 0.749 ± 0.044	0.221TrpMet: 0.221 ± 0.022	0.493TrpAsn: 0.493 ± 0.038	0.171TrpPro: 0.171 ± 0.025	0.268TrpGln: 0.268 ± 0.024	0.185TrpArg: 0.185 ± 0.019	0.345TrpSer: 0.345 ± 0.029	0.394TrpThr: 0.394 ± 0.03	0.448TrpVal: 0.448 ± 0.034	0.089TrpTrp: 0.089 ± 0.016	0.357TrpTyr: 0.357 ± 0.032	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.786TyrAla: 2.786 ± 0.093	0.695TyrCys: 0.695 ± 0.042	3.192TyrAsp: 3.192 ± 0.101	3.262TyrGlu: 3.262 ± 0.1	2.685TyrPhe: 2.685 ± 0.092	2.774TyrGly: 2.774 ± 0.094	1.392TyrHis: 1.392 ± 0.065	3.685TyrIle: 3.685 ± 0.093	2.997TyrLys: 2.997 ± 0.087	4.964TyrLeu: 4.964 ± 0.125	0.786TyrMet: 0.786 ± 0.044	2.344TyrAsn: 2.344 ± 0.081	1.77TyrPro: 1.77 ± 0.067	3.21TyrGln: 3.21 ± 0.112	1.65TyrArg: 1.65 ± 0.056	2.842TyrSer: 2.842 ± 0.083	2.528TyrThr: 2.528 ± 0.09	2.884TyrVal: 2.884 ± 0.071	0.314TyrTrp: 0.314 ± 0.029	2.668TyrTyr: 2.668 ± 0.086	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.005XaaAla: 0.005 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.005XaaGlu: 0.005 ± 0.004	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.002XaaMet: 0.002 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.002	0.002XaaVal: 0.002 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.005XaaTyr: 0.005 ± 0.003	0.04XaaXaa: 0.04 ± 0.013

Statistics based on 1348 proteins (426106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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