Puccinia sorghi

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21032 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6UEM3|A0A0L6UEM3_9BASI Uncharacterized protein OS=Puccinia sorghi OX=27349 GN=VP01_679g1 PE=4 SV=1
CC1 pKa = 7.31FFDD4 pKa = 5.79KK5 pKa = 11.23LIDD8 pKa = 5.4LDD10 pKa = 4.29DD11 pKa = 3.53TDD13 pKa = 3.59YY14 pKa = 11.44WFDD17 pKa = 4.46FDD19 pKa = 5.23VLGCAEE25 pKa = 4.19TRR27 pKa = 11.84NLDD30 pKa = 3.87SIADD34 pKa = 4.19LSCPTNPSPADD45 pKa = 3.89RR46 pKa = 11.84DD47 pKa = 3.82PLLVEE52 pKa = 3.79QDD54 pKa = 3.73PLRR57 pKa = 11.84EE58 pKa = 3.92ARR60 pKa = 11.84QLDD63 pKa = 3.79EE64 pKa = 5.33GVCTSNDD71 pKa = 4.02PIGCSVSEE79 pKa = 4.41TNSTLPAPQYY89 pKa = 11.09CEE91 pKa = 3.7FVLYY95 pKa = 10.95SLVPVSAGQPSPPCEE110 pKa = 3.37IWKK113 pKa = 9.88DD114 pKa = 3.57AEE116 pKa = 4.34IVDD119 pKa = 4.62TICRR123 pKa = 11.84EE124 pKa = 4.17GPADD128 pKa = 3.7RR129 pKa = 11.84TTPRR133 pKa = 3.53

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6V4K4|A0A0L6V4K4_9BASI Uncharacterized protein OS=Puccinia sorghi OX=27349 GN=VP01_2682g3 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84PRR4 pKa = 11.84KK5 pKa = 9.81KK6 pKa = 10.67NGVFLIAFTSGQFIIVAARR25 pKa = 11.84RR26 pKa = 11.84LPRR29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 3.88QKK33 pKa = 10.43HH34 pKa = 5.18KK35 pKa = 10.55SRR37 pKa = 11.84TNRR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 4.96AGEE45 pKa = 4.17RR46 pKa = 11.84KK47 pKa = 9.59LRR49 pKa = 11.84TAWLGEE55 pKa = 3.67GRR57 pKa = 11.84GLLGEE62 pKa = 4.2THH64 pKa = 5.6EE65 pKa = 4.35QEE67 pKa = 4.49HH68 pKa = 6.08LHH70 pKa = 7.06AEE72 pKa = 4.12HH73 pKa = 7.33ADD75 pKa = 3.67NGGEE79 pKa = 3.66EE80 pKa = 4.08HH81 pKa = 7.18FYY83 pKa = 10.66IKK85 pKa = 10.73YY86 pKa = 10.0ILIVVLKK93 pKa = 10.36RR94 pKa = 11.84LVRR97 pKa = 11.84LKK99 pKa = 10.38RR100 pKa = 11.84PMMMEE105 pKa = 4.2SSSHH109 pKa = 5.22GACRR113 pKa = 11.84RR114 pKa = 11.84CCWPIGGKK122 pKa = 10.42AMGSGPTYY130 pKa = 8.12RR131 pKa = 11.84TRR133 pKa = 11.84RR134 pKa = 11.84GSEE137 pKa = 3.75LATCRR142 pKa = 11.84QSDD145 pKa = 4.05PSVKK149 pKa = 9.79TPSQSGLLTCRR160 pKa = 11.84GQCCGLRR167 pKa = 11.84TACHH171 pKa = 6.33SSLSVGGDD179 pKa = 3.4YY180 pKa = 10.92LSPPGHH186 pKa = 6.57SYY188 pKa = 11.41GILNRR193 pKa = 11.84EE194 pKa = 4.63LFPQQLGNTIGTATYY209 pKa = 9.5FDD211 pKa = 4.34SRR213 pKa = 11.84AEE215 pKa = 4.14SPLNALSIGQDD226 pKa = 2.78ASGVVTRR233 pKa = 11.84CLTPQICLDD242 pKa = 3.73VNQKK246 pKa = 8.64STVRR250 pKa = 11.84QLLSNSIRR258 pKa = 11.84PHH260 pKa = 6.38PGRR263 pKa = 11.84GVGSTASGQGKK274 pKa = 7.54WPSGPVVVRR283 pKa = 11.84FDD285 pKa = 3.4IVKK288 pKa = 10.39SVANQATTFDD298 pKa = 3.86VSPPASVIKK307 pKa = 10.31GGRR310 pKa = 11.84PLTVMLISFSLTTRR324 pKa = 11.84PLRR327 pKa = 11.84RR328 pKa = 11.84CFSKK332 pKa = 10.72RR333 pKa = 11.84GFNSSKK339 pKa = 10.43MMLEE343 pKa = 4.56QQDD346 pKa = 3.84SWCGRR351 pKa = 11.84PSHH354 pKa = 6.21QPAPMVSYY362 pKa = 10.06PLVSVGLTAKK372 pKa = 9.22LQKK375 pKa = 9.29MRR377 pKa = 11.84CCAPGSEE384 pKa = 4.04VRR386 pKa = 11.84LRR388 pKa = 11.84AGRR391 pKa = 11.84VTQHH395 pKa = 5.79VSMHH399 pKa = 6.45TILRR403 pKa = 11.84CC404 pKa = 3.6

Molecular weight: 44.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21032

0

21032

7432697

28

3846

353.4

39.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.112 ± 0.019

2.065 ± 0.011

4.348 ± 0.013

5.008 ± 0.016

4.611 ± 0.016

5.309 ± 0.016

3.243 ± 0.011

5.74 ± 0.014

5.897 ± 0.019

10.367 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.006

4.654 ± 0.011

6.179 ± 0.022

4.593 ± 0.012

5.165 ± 0.014

9.686 ± 0.023

5.794 ± 0.01

5.105 ± 0.011

1.433 ± 0.007

2.574 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski