Porphyromonas sp. HMSC065F10
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1615 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1F1E1C0|A0A1F1E1C0_9PORP Uncharacterized protein OS=Porphyromonas sp. HMSC065F10 OX=1739394 GN=HMPREF2890_04790 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.16 LASKK10 pKa = 10.73 LDD12 pKa = 3.97 DD13 pKa = 4.74 APWPATKK20 pKa = 10.54 DD21 pKa = 3.44 EE22 pKa = 5.33 LIDD25 pKa = 3.63 YY26 pKa = 7.94 AQRR29 pKa = 11.84 TCAPMAVIEE38 pKa = 4.12 NLQEE42 pKa = 4.58 IEE44 pKa = 4.8 DD45 pKa = 4.44 DD46 pKa = 4.14 GEE48 pKa = 4.52 SYY50 pKa = 11.21 EE51 pKa = 4.48 SIEE54 pKa = 5.44 DD55 pKa = 3.13 IWPDD59 pKa = 3.64 YY60 pKa = 10.2 PSKK63 pKa = 11.18 EE64 pKa = 3.57 DD65 pKa = 3.4 FFFNEE70 pKa = 3.88 EE71 pKa = 4.02 EE72 pKa = 4.43 YY73 pKa = 11.38
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 3.605
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.656
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.645
Protein with the highest isoelectric point:
>tr|A0A1F1E715|A0A1F1E715_9PORP Potassium transporter TrkA OS=Porphyromonas sp. HMSC065F10 OX=1739394 GN=HMPREF2890_03410 PE=3 SV=1
MM1 pKa = 7.2 SRR3 pKa = 11.84 SRR5 pKa = 11.84 NAIAFGIIAILIVVAFVLNLSLGTIAIPFRR35 pKa = 11.84 DD36 pKa = 3.67 VVDD39 pKa = 3.46 ILLGRR44 pKa = 11.84 DD45 pKa = 3.25 TGGSEE50 pKa = 3.28 IWRR53 pKa = 11.84 NIVWKK58 pKa = 10.32 SRR60 pKa = 11.84 YY61 pKa = 7.95 PQTITALVAGAGLSISGLLMQTVFRR86 pKa = 11.84 NPLAGPSEE94 pKa = 4.38 LGISSGASLGVATLVLLSGSISQRR118 pKa = 11.84 ALIRR122 pKa = 11.84 MGFVGEE128 pKa = 4.21 MAVSVAALLGALAVMGIIMIMSEE151 pKa = 4.18 KK152 pKa = 10.67 VKK154 pKa = 10.98 GNVVLLIMGVMIGYY168 pKa = 9.07 IANAIIGVLKK178 pKa = 10.47 FFSNDD183 pKa = 2.66 EE184 pKa = 4.1 DD185 pKa = 3.62 VRR187 pKa = 11.84 SYY189 pKa = 10.62 VVWGLGSFSKK199 pKa = 10.97 VSGDD203 pKa = 3.24 RR204 pKa = 11.84 VTTFVLLMLVLIPLSFPLIKK224 pKa = 9.8 TLNLLLLGEE233 pKa = 4.42 NYY235 pKa = 10.18 ARR237 pKa = 11.84 SLGLNYY243 pKa = 9.53 KK244 pKa = 9.79 RR245 pKa = 11.84 ARR247 pKa = 11.84 HH248 pKa = 5.14 RR249 pKa = 11.84 VIFCSCLLTAIVTAYY264 pKa = 9.85 CGPIVFIGLAVPHH277 pKa = 6.85 LCRR280 pKa = 11.84 TIFATSNHH288 pKa = 7.2 RR289 pKa = 11.84 ILMPAVTLVGALLALICSVIARR311 pKa = 11.84 MPGFEE316 pKa = 4.06 GALPINSVTALIGAPIVIWVLFSRR340 pKa = 11.84 RR341 pKa = 11.84 RR342 pKa = 11.84 GEE344 pKa = 3.87 RR345 pKa = 11.84 LL346 pKa = 3.0
Molecular weight: 36.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.589
IPC_protein 10.409
Toseland 10.409
ProMoST 10.16
Dawson 10.57
Bjellqvist 10.321
Wikipedia 10.789
Rodwell 10.716
Grimsley 10.643
Solomon 10.657
Lehninger 10.613
Nozaki 10.438
DTASelect 10.306
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.394
IPC_peptide 10.657
IPC2_peptide 9.545
IPC2.peptide.svr19 8.475
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1615
0
1615
593930
38
2001
367.8
41.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.392 ± 0.055
1.007 ± 0.021
6.217 ± 0.038
6.989 ± 0.051
3.697 ± 0.035
7.305 ± 0.046
2.2 ± 0.023
6.075 ± 0.047
4.908 ± 0.057
10.15 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.022
3.187 ± 0.042
4.438 ± 0.036
3.175 ± 0.035
6.355 ± 0.054
6.595 ± 0.053
5.919 ± 0.037
6.624 ± 0.05
1.07 ± 0.021
4.104 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here