Porphyromonas sp. HMSC065F10

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; unclassified Porphyromonas

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1F1E1C0|A0A1F1E1C0_9PORP Uncharacterized protein OS=Porphyromonas sp. HMSC065F10 OX=1739394 GN=HMPREF2890_04790 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.16LASKK10 pKa = 10.73LDD12 pKa = 3.97DD13 pKa = 4.74APWPATKK20 pKa = 10.54DD21 pKa = 3.44EE22 pKa = 5.33LIDD25 pKa = 3.63YY26 pKa = 7.94AQRR29 pKa = 11.84TCAPMAVIEE38 pKa = 4.12NLQEE42 pKa = 4.58IEE44 pKa = 4.8DD45 pKa = 4.44DD46 pKa = 4.14GEE48 pKa = 4.52SYY50 pKa = 11.21EE51 pKa = 4.48SIEE54 pKa = 5.44DD55 pKa = 3.13IWPDD59 pKa = 3.64YY60 pKa = 10.2PSKK63 pKa = 11.18EE64 pKa = 3.57DD65 pKa = 3.4FFFNEE70 pKa = 3.88EE71 pKa = 4.02EE72 pKa = 4.43YY73 pKa = 11.38

Molecular weight:
8.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1F1E715|A0A1F1E715_9PORP Potassium transporter TrkA OS=Porphyromonas sp. HMSC065F10 OX=1739394 GN=HMPREF2890_03410 PE=3 SV=1
MM1 pKa = 7.2SRR3 pKa = 11.84SRR5 pKa = 11.84NAIAFGIIAILIVVAFVLNLSLGTIAIPFRR35 pKa = 11.84DD36 pKa = 3.67VVDD39 pKa = 3.46ILLGRR44 pKa = 11.84DD45 pKa = 3.25TGGSEE50 pKa = 3.28IWRR53 pKa = 11.84NIVWKK58 pKa = 10.32SRR60 pKa = 11.84YY61 pKa = 7.95PQTITALVAGAGLSISGLLMQTVFRR86 pKa = 11.84NPLAGPSEE94 pKa = 4.38LGISSGASLGVATLVLLSGSISQRR118 pKa = 11.84ALIRR122 pKa = 11.84MGFVGEE128 pKa = 4.21MAVSVAALLGALAVMGIIMIMSEE151 pKa = 4.18KK152 pKa = 10.67VKK154 pKa = 10.98GNVVLLIMGVMIGYY168 pKa = 9.07IANAIIGVLKK178 pKa = 10.47FFSNDD183 pKa = 2.66EE184 pKa = 4.1DD185 pKa = 3.62VRR187 pKa = 11.84SYY189 pKa = 10.62VVWGLGSFSKK199 pKa = 10.97VSGDD203 pKa = 3.24RR204 pKa = 11.84VTTFVLLMLVLIPLSFPLIKK224 pKa = 9.8TLNLLLLGEE233 pKa = 4.42NYY235 pKa = 10.18ARR237 pKa = 11.84SLGLNYY243 pKa = 9.53KK244 pKa = 9.79RR245 pKa = 11.84ARR247 pKa = 11.84HH248 pKa = 5.14RR249 pKa = 11.84VIFCSCLLTAIVTAYY264 pKa = 9.85CGPIVFIGLAVPHH277 pKa = 6.85LCRR280 pKa = 11.84TIFATSNHH288 pKa = 7.2RR289 pKa = 11.84ILMPAVTLVGALLALICSVIARR311 pKa = 11.84MPGFEE316 pKa = 4.06GALPINSVTALIGAPIVIWVLFSRR340 pKa = 11.84RR341 pKa = 11.84RR342 pKa = 11.84GEE344 pKa = 3.87RR345 pKa = 11.84LL346 pKa = 3.0

Molecular weight:
36.95 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1615

0

1615

593930

38

2001

367.8

41.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.392 ± 0.055

1.007 ± 0.021

6.217 ± 0.038

6.989 ± 0.051

3.697 ± 0.035

7.305 ± 0.046

2.2 ± 0.023

6.075 ± 0.047

4.908 ± 0.057

10.15 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.022

3.187 ± 0.042

4.438 ± 0.036

3.175 ± 0.035

6.355 ± 0.054

6.595 ± 0.053

5.919 ± 0.037

6.624 ± 0.05

1.07 ± 0.021

4.104 ± 0.039

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski