Clostridium fallax

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4TCC7|A0A1M4TCC7_9CLOT Predicted heme/steroid binding protein OS=Clostridium fallax OX=1533 GN=SAMN05443638_102124 PE=4 SV=1
MM1 pKa = 7.09EE2 pKa = 5.47AKK4 pKa = 9.85VDD6 pKa = 3.69KK7 pKa = 9.7EE8 pKa = 4.09LCISCGLCPTVCPEE22 pKa = 3.74VFEE25 pKa = 4.95IEE27 pKa = 4.25GDD29 pKa = 3.62GKK31 pKa = 10.98AGVLVDD37 pKa = 4.42QIPDD41 pKa = 3.57GVEE44 pKa = 3.72DD45 pKa = 3.69QARR48 pKa = 11.84EE49 pKa = 4.09AQEE52 pKa = 4.36GCPVDD57 pKa = 5.36AIDD60 pKa = 5.04IFF62 pKa = 4.13

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4X5P2|A0A1M4X5P2_9CLOT Coenzyme A biosynthesis bifunctional protein CoaBC OS=Clostridium fallax OX=1533 GN=coaBC PE=3 SV=1
MM1 pKa = 7.16SRR3 pKa = 11.84EE4 pKa = 3.81VRR6 pKa = 11.84KK7 pKa = 9.99GGKK10 pKa = 8.29FRR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 10.8RR16 pKa = 11.84KK17 pKa = 8.3VCAFCVEE24 pKa = 3.91KK25 pKa = 11.26AEE27 pKa = 4.82AIDD30 pKa = 3.69YY31 pKa = 11.09KK32 pKa = 11.11DD33 pKa = 3.47VNKK36 pKa = 10.15LRR38 pKa = 11.84KK39 pKa = 9.37YY40 pKa = 7.57VTEE43 pKa = 4.22RR44 pKa = 11.84GKK46 pKa = 10.15ILPRR50 pKa = 11.84RR51 pKa = 11.84ISGTCAKK58 pKa = 9.97HH59 pKa = 5.27QRR61 pKa = 11.84QLTEE65 pKa = 3.95SIKK68 pKa = 9.95RR69 pKa = 11.84ARR71 pKa = 11.84NIALLPFTTEE81 pKa = 3.59

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2521

0

2521

777836

27

2089

308.5

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.166 ± 0.053

1.153 ± 0.021

5.629 ± 0.04

7.503 ± 0.063

4.578 ± 0.045

6.423 ± 0.046

1.202 ± 0.016

10.466 ± 0.066

10.04 ± 0.057

9.265 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.024

6.805 ± 0.057

2.739 ± 0.029

1.972 ± 0.02

3.177 ± 0.031

6.041 ± 0.039

4.563 ± 0.03

6.122 ± 0.042

0.646 ± 0.019

4.073 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski