Beihai tombus-like virus 17
Average proteome isoelectric point is 7.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFW8|A0A1L3KFW8_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 17 OX=1922720 PE=4 SV=1
MM1 pKa = 7.36 MNTRR5 pKa = 11.84 KK6 pKa = 9.78 QIRR9 pKa = 11.84 NLNFEE14 pKa = 4.94 EE15 pKa = 4.22 LTKK18 pKa = 10.96 ACGCDD23 pKa = 3.18 RR24 pKa = 11.84 DD25 pKa = 3.89 AQNWLKK31 pKa = 10.9 VALDD35 pKa = 3.78 PFHH38 pKa = 7.79 DD39 pKa = 4.36 YY40 pKa = 10.89 NVQLAGLPDD49 pKa = 4.29 NDD51 pKa = 4.4 SQPSAIQIVPKK62 pKa = 10.26 VYY64 pKa = 10.23 QIKK67 pKa = 10.48 APAGLAVNEE76 pKa = 4.29 TWSAHH81 pKa = 5.68 ISTLPIASTHH91 pKa = 5.76 ACRR94 pKa = 11.84 SYY96 pKa = 11.0 YY97 pKa = 10.48 RR98 pKa = 11.84 NNTSGVVGDD107 pKa = 4.26 AAVTLSNDD115 pKa = 3.17 GQAGEE120 pKa = 4.52 IGTITVITHH129 pKa = 6.29 GDD131 pKa = 3.14 AGGLNGYY138 pKa = 10.48 DD139 pKa = 3.42 NLSYY143 pKa = 9.97 PANVAQATDD152 pKa = 4.17 FGAITRR158 pKa = 11.84 EE159 pKa = 4.05 FKK161 pKa = 10.96 AFSLDD166 pKa = 3.58 DD167 pKa = 5.54 DD168 pKa = 4.51 NDD170 pKa = 3.39 KK171 pKa = 10.8 TMKK174 pKa = 10.51 KK175 pKa = 9.65 LVAGGFEE182 pKa = 4.19 VHH184 pKa = 6.72 NDD186 pKa = 2.87 TAALYY191 pKa = 9.15 KK192 pKa = 10.25 QGSVTVYY199 pKa = 10.7 SSGQGTQRR207 pKa = 11.84 EE208 pKa = 4.48 GALLRR213 pKa = 11.84 NADD216 pKa = 3.4 TSTVYY221 pKa = 10.19 HH222 pKa = 6.18 HH223 pKa = 5.08 QHH225 pKa = 5.66 LRR227 pKa = 11.84 KK228 pKa = 9.52 SRR230 pKa = 11.84 QPPGSKK236 pKa = 10.39 AEE238 pKa = 4.31 AGSMPDD244 pKa = 2.79 SRR246 pKa = 11.84 TFEE249 pKa = 4.33 AADD252 pKa = 3.47 GCYY255 pKa = 10.4 VPIRR259 pKa = 11.84 LGDD262 pKa = 3.63 DD263 pKa = 3.19 THH265 pKa = 8.24 YY266 pKa = 11.08 SIASRR271 pKa = 11.84 TTYY274 pKa = 9.33 LTEE277 pKa = 4.28 SVDD280 pKa = 3.48 SSLVTTRR287 pKa = 11.84 PAGYY291 pKa = 10.22 VITPFAATAGYY302 pKa = 10.19 SEE304 pKa = 6.02 DD305 pKa = 3.41 YY306 pKa = 9.95 QANHH310 pKa = 6.95 RR311 pKa = 11.84 PLDD314 pKa = 3.56 IEE316 pKa = 4.14 TTGAYY321 pKa = 10.1 FSGLSPEE328 pKa = 4.29 TTLTLTIKK336 pKa = 10.3 FIIEE340 pKa = 4.33 LCPTSSNQAMLYY352 pKa = 7.85 MASPTAMYY360 pKa = 9.9 CPRR363 pKa = 11.84 AIEE366 pKa = 5.34 LYY368 pKa = 9.39 HH369 pKa = 7.39 QMIRR373 pKa = 11.84 SLPPGVPVSFNDD385 pKa = 3.19 KK386 pKa = 10.92 GDD388 pKa = 3.72 WFRR391 pKa = 11.84 MATKK395 pKa = 10.61 VMADD399 pKa = 3.44 VAPLVSPFLSALNPTLGAGVQLAGQVAKK427 pKa = 9.41 ATNKK431 pKa = 10.31 AISKK435 pKa = 9.92 QEE437 pKa = 3.75 TSVAPNQKK445 pKa = 8.41 STQPPPRR452 pKa = 11.84 TKK454 pKa = 10.42 VVSQPNIGRR463 pKa = 11.84 SVARR467 pKa = 11.84 KK468 pKa = 9.07 KK469 pKa = 10.7
Molecular weight: 50.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.551
IPC2_protein 6.59
IPC_protein 6.737
Toseland 6.81
ProMoST 7.293
Dawson 7.249
Bjellqvist 7.161
Wikipedia 7.22
Rodwell 7.234
Grimsley 6.927
Solomon 7.278
Lehninger 7.293
Nozaki 7.483
DTASelect 7.527
Thurlkill 7.571
EMBOSS 7.6
Sillero 7.644
Patrickios 4.075
IPC_peptide 7.293
IPC2_peptide 7.102
IPC2.peptide.svr19 6.994
Protein with the highest isoelectric point:
>tr|A0A1L3KFW8|A0A1L3KFW8_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 17 OX=1922720 PE=4 SV=1
MM1 pKa = 7.48 GKK3 pKa = 9.8 KK4 pKa = 9.95 KK5 pKa = 10.76 SRR7 pKa = 11.84 FRR9 pKa = 11.84 DD10 pKa = 3.12 KK11 pKa = 11.33 DD12 pKa = 3.66 LLDD15 pKa = 3.72 EE16 pKa = 5.3 RR17 pKa = 11.84 GLGDD21 pKa = 3.65 GPVNEE26 pKa = 4.05 PQKK29 pKa = 10.83 RR30 pKa = 11.84 KK31 pKa = 10.54 DD32 pKa = 3.48 YY33 pKa = 10.81 LRR35 pKa = 11.84 EE36 pKa = 3.76 RR37 pKa = 11.84 NLISFKK43 pKa = 11.21 GEE45 pKa = 3.41 NTGRR49 pKa = 11.84 QNQKK53 pKa = 10.43 FEE55 pKa = 4.88 DD56 pKa = 4.66 DD57 pKa = 3.73 YY58 pKa = 11.9 NSYY61 pKa = 11.04 LKK63 pKa = 10.65 RR64 pKa = 11.84 EE65 pKa = 4.04 PVSNKK70 pKa = 9.32 EE71 pKa = 3.89 KK72 pKa = 10.04 EE73 pKa = 4.42 AKK75 pKa = 10.03 EE76 pKa = 3.64 KK77 pKa = 9.26 TAYY80 pKa = 9.32 NARR83 pKa = 11.84 IRR85 pKa = 11.84 LLRR88 pKa = 11.84 KK89 pKa = 10.14 DD90 pKa = 4.17 KK91 pKa = 10.79 EE92 pKa = 4.35 LTDD95 pKa = 3.9 GKK97 pKa = 9.43 PVKK100 pKa = 10.43 GQIRR104 pKa = 11.84 ILDD107 pKa = 3.69 DD108 pKa = 4.76 KK109 pKa = 10.57 ITVGGSKK116 pKa = 10.19 NVTYY120 pKa = 10.32 AVPGIRR126 pKa = 11.84 RR127 pKa = 11.84 PEE129 pKa = 3.94 TQRR132 pKa = 11.84 VDD134 pKa = 2.87 GGNRR138 pKa = 11.84 MRR140 pKa = 11.84 DD141 pKa = 3.4 RR142 pKa = 11.84 QGKK145 pKa = 6.18 GHH147 pKa = 6.25 RR148 pKa = 11.84 TVVPHH153 pKa = 5.82 PRR155 pKa = 11.84 EE156 pKa = 3.77 YY157 pKa = 10.75 TIVDD161 pKa = 3.51 NSSEE165 pKa = 4.15 MTVTFHH171 pKa = 7.1 GFFSEE176 pKa = 4.54 VVTFTTLPKK185 pKa = 9.48 PHH187 pKa = 6.66 QSRR190 pKa = 11.84 LDD192 pKa = 3.52 RR193 pKa = 11.84 HH194 pKa = 6.61 GGGQDD199 pKa = 3.68 DD200 pKa = 4.37 NDD202 pKa = 3.98 VVYY205 pKa = 9.13 TWDD208 pKa = 3.23 LGRR211 pKa = 11.84 IKK213 pKa = 10.54 RR214 pKa = 11.84 NKK216 pKa = 9.2 LVHH219 pKa = 6.07 ALKK222 pKa = 11.0 GNTPYY227 pKa = 10.48 VWHH230 pKa = 6.57 VPRR233 pKa = 11.84 KK234 pKa = 8.54 EE235 pKa = 3.97 ANKK238 pKa = 9.92 LQHH241 pKa = 6.41 SLNGNTPEE249 pKa = 4.58 AEE251 pKa = 4.17 PKK253 pKa = 10.36 KK254 pKa = 10.48 AVCNTCGKK262 pKa = 9.84 EE263 pKa = 3.92 GHH265 pKa = 6.47 FKK267 pKa = 10.74 RR268 pKa = 11.84 NCPKK272 pKa = 10.66 AGAKK276 pKa = 10.11 GGGRR280 pKa = 11.84 GGDD283 pKa = 3.21 KK284 pKa = 9.73 MLKK287 pKa = 10.05 RR288 pKa = 11.84 GMADD292 pKa = 2.95 MEE294 pKa = 4.3 AQRR297 pKa = 11.84 KK298 pKa = 9.13 AEE300 pKa = 3.96 NDD302 pKa = 2.99 NAKK305 pKa = 10.19 EE306 pKa = 3.94 KK307 pKa = 10.99 RR308 pKa = 11.84 GDD310 pKa = 3.44 QKK312 pKa = 11.22 KK313 pKa = 9.86 EE314 pKa = 3.72 KK315 pKa = 9.95 EE316 pKa = 4.06 AVKK319 pKa = 10.68 CRR321 pKa = 11.84 FFNDD325 pKa = 2.85 NLEE328 pKa = 4.59 PIRR331 pKa = 11.84 FKK333 pKa = 11.55 VEE335 pKa = 3.6 VKK337 pKa = 10.74 EE338 pKa = 4.18 PVTPMSWIRR347 pKa = 11.84 LGFLFRR353 pKa = 11.84 IAPLMMGFFFFDD365 pKa = 3.43 LGLWPHH371 pKa = 6.98 ISNLAATLAEE381 pKa = 4.29 HH382 pKa = 5.94 VVHH385 pKa = 6.96 VILFLPVFVGMMMLEE400 pKa = 4.36 NIFVGLVAYY409 pKa = 9.13 RR410 pKa = 11.84 AKK412 pKa = 10.8 AVFTFFLGKK421 pKa = 8.84 LTVRR425 pKa = 11.84 HH426 pKa = 6.5 SIALYY431 pKa = 8.5 PVPDD435 pKa = 3.33 HH436 pKa = 7.4 RR437 pKa = 11.84 EE438 pKa = 3.67 FSVEE442 pKa = 3.61 EE443 pKa = 4.08 DD444 pKa = 4.27 KK445 pKa = 11.49 RR446 pKa = 11.84 PLDD449 pKa = 3.6 CTFGPRR455 pKa = 11.84 AYY457 pKa = 10.44 VAGICRR463 pKa = 11.84 YY464 pKa = 7.55 TVTEE468 pKa = 3.97 CFYY471 pKa = 11.52 VSFPYY476 pKa = 9.32 WQIHH480 pKa = 4.7 MALGSRR486 pKa = 11.84 RR487 pKa = 11.84 LAVRR491 pKa = 11.84 EE492 pKa = 4.08 CSSTAIALYY501 pKa = 10.59 GSVINSNLTVEE512 pKa = 5.07 EE513 pKa = 3.93 YY514 pKa = 10.8 RR515 pKa = 11.84 DD516 pKa = 3.77 AIRR519 pKa = 11.84 RR520 pKa = 11.84 NLSKK524 pKa = 10.48 CSKK527 pKa = 9.74 IDD529 pKa = 3.09 WDD531 pKa = 3.98 AEE533 pKa = 3.77 EE534 pKa = 4.61 MIRR537 pKa = 11.84 GNILEE542 pKa = 4.3 NTCSYY547 pKa = 11.43 LEE549 pKa = 3.93 YY550 pKa = 10.88 LKK552 pKa = 11.21 LKK554 pKa = 10.18 VDD556 pKa = 3.42 QGDD559 pKa = 3.57 FDD561 pKa = 4.43 KK562 pKa = 11.45 SLEE565 pKa = 3.96 NRR567 pKa = 11.84 SYY569 pKa = 11.74 EE570 pKa = 4.72 DD571 pKa = 2.94 VDD573 pKa = 5.55 LFDD576 pKa = 5.69 APWFHH581 pKa = 6.55 TKK583 pKa = 10.14 AGRR586 pKa = 11.84 IFSVLFVLGLVYY598 pKa = 10.61 FGGGTLASIVMALFRR613 pKa = 11.84 VVAAVWTAISAFMVQCNEE631 pKa = 3.82 FAGSIASLRR640 pKa = 11.84 PLGVYY645 pKa = 9.98 GYY647 pKa = 10.06 SVSDD651 pKa = 4.36 PIFQNVSINEE661 pKa = 3.81 VDD663 pKa = 3.5 PEE665 pKa = 4.21 KK666 pKa = 10.67 FLSFTSIFPALNKK679 pKa = 8.89 TRR681 pKa = 11.84 AVMATFSPFAIPGIALPMTDD701 pKa = 4.23 RR702 pKa = 11.84 GDD704 pKa = 3.84 MLTTAAGLIHH714 pKa = 7.19 RR715 pKa = 11.84 GAALTPKK722 pKa = 9.82 VDD724 pKa = 4.78 KK725 pKa = 10.23 IWNRR729 pKa = 11.84 AKK731 pKa = 10.68 RR732 pKa = 11.84 EE733 pKa = 3.96 IVDD736 pKa = 3.78 QIVDD740 pKa = 3.77 RR741 pKa = 11.84 FFLPLSDD748 pKa = 4.04 SDD750 pKa = 5.66 LIGFDD755 pKa = 4.22 EE756 pKa = 5.48 MIDD759 pKa = 3.61 NTNYY763 pKa = 9.78 TLDD766 pKa = 3.4 RR767 pKa = 11.84 KK768 pKa = 10.33 KK769 pKa = 10.55 QIKK772 pKa = 7.58 TWHH775 pKa = 6.76 KK776 pKa = 10.05 NRR778 pKa = 11.84 KK779 pKa = 9.01 SDD781 pKa = 3.52 FARR784 pKa = 11.84 PTKK787 pKa = 10.48 HH788 pKa = 6.72 KK789 pKa = 8.52 EE790 pKa = 3.47 TGMFNKK796 pKa = 10.13 EE797 pKa = 3.46 EE798 pKa = 4.58 PYY800 pKa = 10.86 SVLKK804 pKa = 9.21 QARR807 pKa = 11.84 SIQGMYY813 pKa = 10.4 RR814 pKa = 11.84 SLLEE818 pKa = 3.92 SDD820 pKa = 3.21 VWGSVGRR827 pKa = 11.84 LVHH830 pKa = 6.03 TVQKK834 pKa = 9.21 TVYY837 pKa = 9.59 SLPPSIKK844 pKa = 10.32 KK845 pKa = 10.03 LDD847 pKa = 3.61 PQGIVDD853 pKa = 4.5 KK854 pKa = 10.73 LLSLGPGSKK863 pKa = 8.77 TVSDD867 pKa = 4.01 YY868 pKa = 11.76 SSYY871 pKa = 10.36 EE872 pKa = 3.63 ASFKK876 pKa = 9.98 EE877 pKa = 4.59 TVKK880 pKa = 10.53 EE881 pKa = 3.65 AAQFRR886 pKa = 11.84 LYY888 pKa = 11.11 DD889 pKa = 3.68 RR890 pKa = 11.84 LTEE893 pKa = 4.08 NLSGYY898 pKa = 9.81 AKK900 pKa = 9.73 QVRR903 pKa = 11.84 GQARR907 pKa = 11.84 WVLGRR912 pKa = 11.84 EE913 pKa = 3.74 VSVRR917 pKa = 11.84 NKK919 pKa = 10.01 FFNASIKK926 pKa = 10.39 NIKK929 pKa = 10.15 CSGDD933 pKa = 3.19 FDD935 pKa = 4.19 TSLSNWFDD943 pKa = 4.19 NVATWLTVFEE953 pKa = 4.72 MKK955 pKa = 10.48 HH956 pKa = 4.79 GVHH959 pKa = 5.61 WTDD962 pKa = 3.91 AINWILCEE970 pKa = 4.21 GDD972 pKa = 5.28 DD973 pKa = 5.87 NITDD977 pKa = 3.41 DD978 pKa = 4.59 HH979 pKa = 7.03 GYY981 pKa = 8.13 TFEE984 pKa = 5.86 KK985 pKa = 10.78 EE986 pKa = 4.32 DD987 pKa = 3.75 FAKK990 pKa = 10.79 LGMTAKK996 pKa = 10.05 IEE998 pKa = 4.34 SGLEE1002 pKa = 3.55 LSEE1005 pKa = 5.84 AGFCQKK1011 pKa = 10.2 YY1012 pKa = 9.86 VNVSTGTLIGDD1023 pKa = 4.27 VITFLGKK1030 pKa = 9.88 RR1031 pKa = 11.84 QYY1033 pKa = 11.36 LPARR1037 pKa = 11.84 YY1038 pKa = 8.93 QNSSQVTKK1046 pKa = 10.96 LSLSKK1051 pKa = 9.69 ATAMSVLSMFKK1062 pKa = 9.95 NAPGISEE1069 pKa = 3.87 WAYY1072 pKa = 10.03 KK1073 pKa = 10.32 VLEE1076 pKa = 4.31 LTSGITVRR1084 pKa = 11.84 AKK1086 pKa = 10.57 HH1087 pKa = 5.81 LVAEE1091 pKa = 4.49 VDD1093 pKa = 3.41 KK1094 pKa = 10.51 HH1095 pKa = 5.18 AHH1097 pKa = 5.84 SGTGVKK1103 pKa = 10.6 LSTLFTKK1110 pKa = 10.35 PVILDD1115 pKa = 3.12 SDD1117 pKa = 4.44 RR1118 pKa = 11.84 IMVSRR1123 pKa = 11.84 VFGFTLEE1130 pKa = 4.04 QQAILTTALDD1140 pKa = 3.42 AWTGGPLQLPLSWFPDD1156 pKa = 3.36 LWRR1159 pKa = 11.84 DD1160 pKa = 3.76 FYY1162 pKa = 11.75 GGYY1165 pKa = 8.08 NTLLVGPSHH1174 pKa = 6.79 EE1175 pKa = 4.89 FFNEE1179 pKa = 3.75 TNTISEE1185 pKa = 4.51 LRR1187 pKa = 11.84 RR1188 pKa = 11.84 KK1189 pKa = 9.82 FPEE1192 pKa = 4.04 LLEE1195 pKa = 4.26
Molecular weight: 135.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.589
IPC2_protein 8.478
IPC_protein 8.331
Toseland 9.092
ProMoST 8.916
Dawson 9.399
Bjellqvist 9.194
Wikipedia 9.545
Rodwell 9.648
Grimsley 9.443
Solomon 9.428
Lehninger 9.385
Nozaki 9.326
DTASelect 9.107
Thurlkill 9.268
EMBOSS 9.56
Sillero 9.399
Patrickios 4.902
IPC_peptide 9.414
IPC2_peptide 7.761
IPC2.peptide.svr19 7.666
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1664
469
1195
832.0
93.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.572 ± 1.67
1.202 ± 0.039
5.889 ± 0.067
5.228 ± 0.919
4.808 ± 1.029
7.212 ± 0.089
2.284 ± 0.031
4.627 ± 0.076
6.851 ± 1.092
8.293 ± 0.636
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.224 ± 0.046
4.567 ± 0.17
4.748 ± 0.726
3.185 ± 0.761
5.889 ± 0.714
6.851 ± 0.633
6.731 ± 1.017
7.091 ± 0.351
1.382 ± 0.375
3.365 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here