Ambe virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Ambe phlebovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SHU8|A0A1S5SHU8_9VIRU Nucleoprotein OS=Ambe virus OX=1926500 PE=3 SV=1
MM1 pKa = 7.41NKK3 pKa = 9.84FYY5 pKa = 10.6LYY7 pKa = 10.96DD8 pKa = 3.49KK9 pKa = 10.38MSIIRR14 pKa = 11.84PGGLYY19 pKa = 10.45CSLPRR24 pKa = 11.84VTFEE28 pKa = 5.08AFNAYY33 pKa = 9.1QEE35 pKa = 4.48NPISRR40 pKa = 11.84YY41 pKa = 9.75SDD43 pKa = 3.02IEE45 pKa = 4.12FPLPAGFRR53 pKa = 11.84VSSKK57 pKa = 9.09TRR59 pKa = 11.84ATLSDD64 pKa = 3.71YY65 pKa = 11.43YY66 pKa = 10.35MNRR69 pKa = 11.84EE70 pKa = 3.86FPMHH74 pKa = 6.79WGCSQQSYY82 pKa = 11.07ALAPYY87 pKa = 8.5ITVFDD92 pKa = 4.2EE93 pKa = 4.67MIEE96 pKa = 4.22CLSHH100 pKa = 6.84FKK102 pKa = 10.27EE103 pKa = 4.42HH104 pKa = 6.82EE105 pKa = 4.0ISAEE109 pKa = 3.94CHH111 pKa = 6.07PNLHH115 pKa = 6.66EE116 pKa = 4.47ALSWPTGGPNLDD128 pKa = 3.33YY129 pKa = 10.87MRR131 pKa = 11.84FYY133 pKa = 11.28SNKK136 pKa = 8.08PDD138 pKa = 3.14CMYY141 pKa = 10.73EE142 pKa = 4.2RR143 pKa = 11.84KK144 pKa = 9.84CRR146 pKa = 11.84EE147 pKa = 3.46ATLILRR153 pKa = 11.84ASRR156 pKa = 11.84PNWWFAEE163 pKa = 4.02SLYY166 pKa = 10.78NSHH169 pKa = 6.93KK170 pKa = 10.38NAFHH174 pKa = 6.49EE175 pKa = 4.7SVVRR179 pKa = 11.84GLDD182 pKa = 3.15PRR184 pKa = 11.84FFNGSDD190 pKa = 3.63VIKK193 pKa = 9.67EE194 pKa = 3.8ICIVQCVRR202 pKa = 11.84LLNAVLFDD210 pKa = 4.65GIYY213 pKa = 10.61SRR215 pKa = 11.84VPNKK219 pKa = 9.96FYY221 pKa = 10.42MAVEE225 pKa = 3.79YY226 pKa = 10.46FRR228 pKa = 11.84NKK230 pKa = 10.34LEE232 pKa = 4.18HH233 pKa = 6.95LGPAILGNRR242 pKa = 11.84KK243 pKa = 8.98WIPHH247 pKa = 4.66VNTRR251 pKa = 11.84FDD253 pKa = 4.62LLNKK257 pKa = 10.39CIDD260 pKa = 3.73EE261 pKa = 4.33EE262 pKa = 4.63FCTDD266 pKa = 3.28SVYY269 pKa = 10.98EE270 pKa = 4.17SDD272 pKa = 5.16LDD274 pKa = 3.92LAKK277 pKa = 10.67EE278 pKa = 4.16FEE280 pKa = 5.97DD281 pKa = 3.24ILKK284 pKa = 10.68DD285 pKa = 3.62VTEE288 pKa = 4.3LL289 pKa = 3.64

Molecular weight: 33.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SHU8|A0A1S5SHU8_9VIRU Nucleoprotein OS=Ambe virus OX=1926500 PE=3 SV=1
MM1 pKa = 7.8TDD3 pKa = 3.44YY4 pKa = 10.82QALAIEE10 pKa = 4.66FANAGVSPAEE20 pKa = 3.68IQDD23 pKa = 3.46WVRR26 pKa = 11.84EE27 pKa = 3.97FAYY30 pKa = 10.46QGFDD34 pKa = 2.66AHH36 pKa = 7.34RR37 pKa = 11.84IIEE40 pKa = 4.16LLRR43 pKa = 11.84TLGGDD48 pKa = 2.96AWQADD53 pKa = 4.1ARR55 pKa = 11.84RR56 pKa = 11.84MIVLGLTRR64 pKa = 11.84GNKK67 pKa = 8.66LEE69 pKa = 4.02KK70 pKa = 9.67MVMRR74 pKa = 11.84MSDD77 pKa = 2.95KK78 pKa = 10.92GKK80 pKa = 9.86QATADD85 pKa = 3.64LVRR88 pKa = 11.84KK89 pKa = 9.86YY90 pKa = 10.37KK91 pKa = 10.65LKK93 pKa = 10.72SGNPSRR99 pKa = 11.84DD100 pKa = 3.28EE101 pKa = 3.99LTLSRR106 pKa = 11.84VAAALAGWTCQALVAVQEE124 pKa = 4.33HH125 pKa = 6.26LPITGSYY132 pKa = 8.43MDD134 pKa = 4.61SVSPGYY140 pKa = 8.75PRR142 pKa = 11.84QMMHH146 pKa = 7.0PSFAGLVDD154 pKa = 3.21PTIPQTHH161 pKa = 7.46LDD163 pKa = 3.91TVLEE167 pKa = 4.03AHH169 pKa = 6.47SLYY172 pKa = 10.56LVHH175 pKa = 7.3FSRR178 pKa = 11.84MINPEE183 pKa = 3.45LRR185 pKa = 11.84GRR187 pKa = 11.84PRR189 pKa = 11.84SEE191 pKa = 3.78VVATFRR197 pKa = 11.84QPMLAAVNSNFITPEE212 pKa = 3.51QRR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 9.81FLVSFGIVNSNGVPSAQVTAAANVFKK242 pKa = 11.13AMPP245 pKa = 4.23

Molecular weight: 27.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3997

245

2087

999.3

113.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.154 ± 1.02

2.602 ± 0.721

5.154 ± 0.65

7.105 ± 0.438

5.279 ± 0.313

5.554 ± 0.248

2.627 ± 0.075

6.48 ± 0.476

6.805 ± 0.712

9.057 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.177 ± 0.494

4.528 ± 0.361

3.728 ± 0.529

3.227 ± 0.306

4.929 ± 0.537

8.982 ± 0.693

5.504 ± 0.566

6.28 ± 0.285

1.176 ± 0.154

2.652 ± 0.518
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski