Moraxella phage Mcat19
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PIP6|A0A0R6PIP6_9CAUD Uncharacterized protein OS=Moraxella phage Mcat19 OX=1647534 PE=4 SV=1
MM1 pKa = 6.86 YY2 pKa = 10.44 KK3 pKa = 10.09 KK4 pKa = 10.65 VQTEE8 pKa = 3.69 EE9 pKa = 3.75 DD10 pKa = 3.83 TILNNSAYY18 pKa = 10.23 SILYY22 pKa = 8.48 SVCDD26 pKa = 3.82 EE27 pKa = 4.35 NGEE30 pKa = 4.25 PMFTEE35 pKa = 4.53 ADD37 pKa = 3.78 LPQIAQIDD45 pKa = 3.82 AGTVGEE51 pKa = 5.16 LMDD54 pKa = 5.98 AIKK57 pKa = 10.44 KK58 pKa = 9.09 VNRR61 pKa = 11.84 FDD63 pKa = 3.66 EE64 pKa = 5.05 DD65 pKa = 3.43 EE66 pKa = 5.28 AEE68 pKa = 4.16 LEE70 pKa = 4.42 KK71 pKa = 11.35 NSS73 pKa = 3.69
Molecular weight: 8.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.225
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.732
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.151
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.024
Patrickios 3.503
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.946
Protein with the highest isoelectric point:
>tr|A0A0R6PIL8|A0A0R6PIL8_9CAUD Integrase OS=Moraxella phage Mcat19 OX=1647534 PE=3 SV=1
MM1 pKa = 7.02 YY2 pKa = 10.4 GGWSHH7 pKa = 6.29 EE8 pKa = 4.38 KK9 pKa = 9.93 IAVRR13 pKa = 11.84 YY14 pKa = 9.1 LSAIGYY20 pKa = 8.4 RR21 pKa = 11.84 GKK23 pKa = 10.63 SRR25 pKa = 11.84 ASRR28 pKa = 11.84 HH29 pKa = 5.37 DD30 pKa = 3.4 VRR32 pKa = 11.84 SALIKK37 pKa = 10.73 AEE39 pKa = 4.06 SFLAGLLHH47 pKa = 6.76 KK48 pKa = 10.43 LYY50 pKa = 11.03 SFTT53 pKa = 4.73
Molecular weight: 6.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.26
IPC2_protein 9.911
IPC_protein 10.394
Toseland 10.292
ProMoST 10.043
Dawson 10.496
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.833
Grimsley 10.584
Solomon 10.54
Lehninger 10.511
Nozaki 10.248
DTASelect 10.204
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.599
IPC_peptide 10.54
IPC2_peptide 8.975
IPC2.peptide.svr19 8.609
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
41
0
41
7809
37
1462
190.5
21.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.695 ± 0.981
1.088 ± 0.268
6.262 ± 0.378
6.134 ± 0.25
3.611 ± 0.183
6.864 ± 0.366
2.087 ± 0.253
6.108 ± 0.254
7.479 ± 0.339
9.066 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.702 ± 0.21
4.853 ± 0.246
3.061 ± 0.403
4.405 ± 0.238
4.93 ± 0.254
6.057 ± 0.351
5.583 ± 0.298
6.108 ± 0.272
1.255 ± 0.146
3.65 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here