Microbacterium phage Koji

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kojivirus; Microbacterium virus Koji

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CFE4|A0A2P1CFE4_9CAUD Uncharacterized protein OS=Microbacterium phage Koji OX=2099625 GN=53 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 4.23NTRR5 pKa = 11.84LKK7 pKa = 11.08LSGTTWSEE15 pKa = 3.51LLGIVIPTVITVPSGEE31 pKa = 3.87QFRR34 pKa = 11.84WDD36 pKa = 3.61EE37 pKa = 4.43SVGSYY42 pKa = 10.71CSDD45 pKa = 3.04VTGAGLYY52 pKa = 9.78IEE54 pKa = 5.15PSDD57 pKa = 4.46DD58 pKa = 3.73EE59 pKa = 5.62GNITPDD65 pKa = 2.67SYY67 pKa = 12.07YY68 pKa = 10.55EE69 pKa = 4.01LPEE72 pKa = 3.98PQGDD76 pKa = 3.64INGG79 pKa = 3.71

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CG32|A0A2P1CG32_9CAUD DNA polymerase I OS=Microbacterium phage Koji OX=2099625 GN=37 PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 5.03KK3 pKa = 10.95ALKK6 pKa = 9.06QVKK9 pKa = 10.39AEE11 pKa = 3.98DD12 pKa = 3.7LQRR15 pKa = 11.84GQVIIDD21 pKa = 3.59PEE23 pKa = 4.54GNQARR28 pKa = 11.84VIRR31 pKa = 11.84VRR33 pKa = 11.84RR34 pKa = 11.84VDD36 pKa = 3.29HH37 pKa = 5.28QRR39 pKa = 11.84GRR41 pKa = 11.84LEE43 pKa = 3.84TDD45 pKa = 3.27LGVAVVPLGQRR56 pKa = 11.84FPVLL60 pKa = 3.27

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12811

46

807

228.8

25.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.077 ± 0.457

0.687 ± 0.111

6.479 ± 0.273

6.167 ± 0.458

3.044 ± 0.184

8.227 ± 0.342

1.842 ± 0.204

4.582 ± 0.404

4.723 ± 0.432

8.469 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.098

3.356 ± 0.211

4.754 ± 0.22

4.184 ± 0.177

5.862 ± 0.356

5.854 ± 0.234

6.814 ± 0.305

7.548 ± 0.352

2.061 ± 0.244

2.974 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski