Amino acid dipepetide frequency for Nocardioides sp. HDW12A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.684AlaAla: 18.684 ± 0.189
1.058AlaCys: 1.058 ± 0.036
8.411AlaAsp: 8.411 ± 0.096
7.757AlaGlu: 7.757 ± 0.104
3.604AlaPhe: 3.604 ± 0.062
11.954AlaGly: 11.954 ± 0.118
2.579AlaHis: 2.579 ± 0.05
4.646AlaIle: 4.646 ± 0.077
2.832AlaLys: 2.832 ± 0.077
13.21AlaLeu: 13.21 ± 0.161
2.857AlaMet: 2.857 ± 0.058
2.154AlaAsn: 2.154 ± 0.042
6.044AlaPro: 6.044 ± 0.084
3.701AlaGln: 3.701 ± 0.056
9.167AlaArg: 9.167 ± 0.097
6.741AlaSer: 6.741 ± 0.087
7.573AlaThr: 7.573 ± 0.092
11.061AlaVal: 11.061 ± 0.121
1.864AlaTrp: 1.864 ± 0.041
2.324AlaTyr: 2.324 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.913CysAla: 0.913 ± 0.03
0.082CysCys: 0.082 ± 0.008
0.533CysAsp: 0.533 ± 0.022
0.445CysGlu: 0.445 ± 0.021
0.242CysPhe: 0.242 ± 0.015
0.867CysGly: 0.867 ± 0.029
0.194CysHis: 0.194 ± 0.013
0.197CysIle: 0.197 ± 0.014
0.127CysLys: 0.127 ± 0.011
0.687CysLeu: 0.687 ± 0.025
0.116CysMet: 0.116 ± 0.01
0.149CysAsn: 0.149 ± 0.013
0.467CysPro: 0.467 ± 0.023
0.199CysGln: 0.199 ± 0.013
0.578CysArg: 0.578 ± 0.024
0.536CysSer: 0.536 ± 0.023
0.46CysThr: 0.46 ± 0.022
0.655CysVal: 0.655 ± 0.027
0.115CysTrp: 0.115 ± 0.012
0.132CysTyr: 0.132 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.689AspAla: 7.689 ± 0.095
0.417AspCys: 0.417 ± 0.022
4.669AspAsp: 4.669 ± 0.081
4.654AspGlu: 4.654 ± 0.074
1.851AspPhe: 1.851 ± 0.042
6.125AspGly: 6.125 ± 0.088
1.571AspHis: 1.571 ± 0.038
2.212AspIle: 2.212 ± 0.044
1.344AspLys: 1.344 ± 0.036
7.336AspLeu: 7.336 ± 0.091
0.984AspMet: 0.984 ± 0.028
1.19AspAsn: 1.19 ± 0.034
4.471AspPro: 4.471 ± 0.08
1.908AspGln: 1.908 ± 0.044
4.767AspArg: 4.767 ± 0.075
2.819AspSer: 2.819 ± 0.051
2.912AspThr: 2.912 ± 0.057
6.252AspVal: 6.252 ± 0.078
1.056AspTrp: 1.056 ± 0.034
1.302AspTyr: 1.302 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
7.081GluAla: 7.081 ± 0.098
0.388GluCys: 0.388 ± 0.018
3.066GluAsp: 3.066 ± 0.054
3.368GluGlu: 3.368 ± 0.064
1.576GluPhe: 1.576 ± 0.038
4.338GluGly: 4.338 ± 0.069
1.671GluHis: 1.671 ± 0.046
2.579GluIle: 2.579 ± 0.052
1.405GluLys: 1.405 ± 0.046
6.514GluLeu: 6.514 ± 0.087
1.068GluMet: 1.068 ± 0.034
0.892GluAsn: 0.892 ± 0.028
3.208GluPro: 3.208 ± 0.067
2.326GluGln: 2.326 ± 0.047
5.048GluArg: 5.048 ± 0.069
3.034GluSer: 3.034 ± 0.059
3.124GluThr: 3.124 ± 0.058
5.565GluVal: 5.565 ± 0.081
0.841GluTrp: 0.841 ± 0.029
0.786GluTyr: 0.786 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.826PheAla: 3.826 ± 0.065
0.287PheCys: 0.287 ± 0.017
2.226PheAsp: 2.226 ± 0.047
1.777PheGlu: 1.777 ± 0.041
0.964PhePhe: 0.964 ± 0.03
3.094PheGly: 3.094 ± 0.059
0.609PheHis: 0.609 ± 0.023
0.918PheIle: 0.918 ± 0.031
0.534PheLys: 0.534 ± 0.024
2.582PheLeu: 2.582 ± 0.058
0.498PheMet: 0.498 ± 0.019
0.64PheAsn: 0.64 ± 0.023
1.29PhePro: 1.29 ± 0.037
0.622PheGln: 0.622 ± 0.026
1.687PheArg: 1.687 ± 0.043
1.571PheSer: 1.571 ± 0.039
1.931PheThr: 1.931 ± 0.044
2.776PheVal: 2.776 ± 0.053
0.4PheTrp: 0.4 ± 0.019
0.591PheTyr: 0.591 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
10.174GlyAla: 10.174 ± 0.117
0.832GlyCys: 0.832 ± 0.026
5.549GlyAsp: 5.549 ± 0.078
4.988GlyGlu: 4.988 ± 0.074
3.047GlyPhe: 3.047 ± 0.053
7.739GlyGly: 7.739 ± 0.091
2.17GlyHis: 2.17 ± 0.045
3.711GlyIle: 3.711 ± 0.054
2.249GlyLys: 2.249 ± 0.051
9.261GlyLeu: 9.261 ± 0.099
2.062GlyMet: 2.062 ± 0.042
1.738GlyAsn: 1.738 ± 0.047
4.283GlyPro: 4.283 ± 0.07
2.721GlyGln: 2.721 ± 0.057
6.969GlyArg: 6.969 ± 0.083
5.579GlySer: 5.579 ± 0.08
5.277GlyThr: 5.277 ± 0.084
7.975GlyVal: 7.975 ± 0.103
1.742GlyTrp: 1.742 ± 0.038
2.016GlyTyr: 2.016 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.705HisAla: 2.705 ± 0.057
0.2HisCys: 0.2 ± 0.013
1.619HisAsp: 1.619 ± 0.044
1.354HisGlu: 1.354 ± 0.033
0.604HisPhe: 0.604 ± 0.023
2.17HisGly: 2.17 ± 0.046
0.734HisHis: 0.734 ± 0.029
0.597HisIle: 0.597 ± 0.023
0.388HisLys: 0.388 ± 0.019
2.413HisLeu: 2.413 ± 0.053
0.348HisMet: 0.348 ± 0.019
0.367HisAsn: 0.367 ± 0.02
1.556HisPro: 1.556 ± 0.036
0.64HisGln: 0.64 ± 0.023
1.762HisArg: 1.762 ± 0.04
1.007HisSer: 1.007 ± 0.028
1.165HisThr: 1.165 ± 0.037
2.036HisVal: 2.036 ± 0.042
0.349HisTrp: 0.349 ± 0.018
0.481HisTyr: 0.481 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.033IleAla: 5.033 ± 0.072
0.311IleCys: 0.311 ± 0.018
2.978IleAsp: 2.978 ± 0.051
2.567IleGlu: 2.567 ± 0.055
0.867IlePhe: 0.867 ± 0.031
3.803IleGly: 3.803 ± 0.065
0.738IleHis: 0.738 ± 0.027
1.174IleIle: 1.174 ± 0.038
0.904IleLys: 0.904 ± 0.035
2.752IleLeu: 2.752 ± 0.059
0.514IleMet: 0.514 ± 0.022
0.925IleAsn: 0.925 ± 0.031
1.916IlePro: 1.916 ± 0.041
0.809IleGln: 0.809 ± 0.027
2.374IleArg: 2.374 ± 0.051
2.099IleSer: 2.099 ± 0.045
2.403IleThr: 2.403 ± 0.052
3.367IleVal: 3.367 ± 0.055
0.452IleTrp: 0.452 ± 0.019
0.627IleTyr: 0.627 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
2.668LysAla: 2.668 ± 0.06
0.117LysCys: 0.117 ± 0.012
1.246LysAsp: 1.246 ± 0.038
1.073LysGlu: 1.073 ± 0.037
0.561LysPhe: 0.561 ± 0.026
1.706LysGly: 1.706 ± 0.045
0.521LysHis: 0.521 ± 0.02
0.903LysIle: 0.903 ± 0.027
0.889LysLys: 0.889 ± 0.053
1.868LysLeu: 1.868 ± 0.053
0.435LysMet: 0.435 ± 0.021
0.479LysAsn: 0.479 ± 0.025
1.322LysPro: 1.322 ± 0.04
0.804LysGln: 0.804 ± 0.026
1.565LysArg: 1.565 ± 0.044
1.285LysSer: 1.285 ± 0.044
1.33LysThr: 1.33 ± 0.04
2.159LysVal: 2.159 ± 0.053
0.277LysTrp: 0.277 ± 0.017
0.423LysTyr: 0.423 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.868LeuAla: 14.868 ± 0.143
0.703LeuCys: 0.703 ± 0.027
7.263LeuAsp: 7.263 ± 0.088
5.518LeuGlu: 5.518 ± 0.093
2.523LeuPhe: 2.523 ± 0.051
9.509LeuGly: 9.509 ± 0.104
2.086LeuHis: 2.086 ± 0.047
3.29LeuIle: 3.29 ± 0.069
1.898LeuLys: 1.898 ± 0.047
10.451LeuLeu: 10.451 ± 0.137
1.734LeuMet: 1.734 ± 0.036
1.64LeuAsn: 1.64 ± 0.041
5.586LeuPro: 5.586 ± 0.079
2.314LeuGln: 2.314 ± 0.047
7.405LeuArg: 7.405 ± 0.099
5.407LeuSer: 5.407 ± 0.069
6.353LeuThr: 6.353 ± 0.071
10.519LeuVal: 10.519 ± 0.116
1.108LeuTrp: 1.108 ± 0.039
1.415LeuTyr: 1.415 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.487MetAla: 2.487 ± 0.045
0.144MetCys: 0.144 ± 0.011
0.988MetAsp: 0.988 ± 0.036
0.817MetGlu: 0.817 ± 0.029
0.52MetPhe: 0.52 ± 0.027
1.527MetGly: 1.527 ± 0.042
0.387MetHis: 0.387 ± 0.021
0.707MetIle: 0.707 ± 0.026
0.514MetLys: 0.514 ± 0.02
2.024MetLeu: 2.024 ± 0.043
0.404MetMet: 0.404 ± 0.025
0.415MetAsn: 0.415 ± 0.022
1.162MetPro: 1.162 ± 0.034
0.504MetGln: 0.504 ± 0.023
1.514MetArg: 1.514 ± 0.041
1.594MetSer: 1.594 ± 0.035
1.731MetThr: 1.731 ± 0.042
1.641MetVal: 1.641 ± 0.037
0.232MetTrp: 0.232 ± 0.015
0.274MetTyr: 0.274 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.213AsnAla: 2.213 ± 0.049
0.144AsnCys: 0.144 ± 0.013
1.201AsnAsp: 1.201 ± 0.039
0.959AsnGlu: 0.959 ± 0.03
0.511AsnPhe: 0.511 ± 0.023
1.732AsnGly: 1.732 ± 0.045
0.411AsnHis: 0.411 ± 0.02
0.722AsnIle: 0.722 ± 0.027
0.418AsnLys: 0.418 ± 0.02
1.842AsnLeu: 1.842 ± 0.036
0.315AsnMet: 0.315 ± 0.017
0.473AsnAsn: 0.473 ± 0.023
1.409AsnPro: 1.409 ± 0.038
0.533AsnGln: 0.533 ± 0.021
1.22AsnArg: 1.22 ± 0.035
0.894AsnSer: 0.894 ± 0.028
1.057AsnThr: 1.057 ± 0.035
1.543AsnVal: 1.543 ± 0.044
0.262AsnTrp: 0.262 ± 0.015
0.391AsnTyr: 0.391 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
7.034ProAla: 7.034 ± 0.103
0.328ProCys: 0.328 ± 0.018
4.376ProAsp: 4.376 ± 0.067
3.809ProGlu: 3.809 ± 0.067
1.443ProPhe: 1.443 ± 0.036
5.435ProGly: 5.435 ± 0.083
1.187ProHis: 1.187 ± 0.032
1.768ProIle: 1.768 ± 0.043
1.034ProLys: 1.034 ± 0.033
4.698ProLeu: 4.698 ± 0.058
1.124ProMet: 1.124 ± 0.039
0.858ProAsn: 0.858 ± 0.029
2.787ProPro: 2.787 ± 0.063
1.454ProGln: 1.454 ± 0.038
3.65ProArg: 3.65 ± 0.064
3.215ProSer: 3.215 ± 0.061
3.705ProThr: 3.705 ± 0.071
5.09ProVal: 5.09 ± 0.069
0.879ProTrp: 0.879 ± 0.032
1.087ProTyr: 1.087 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.682GlnAla: 3.682 ± 0.067
0.157GlnCys: 0.157 ± 0.013
1.398GlnAsp: 1.398 ± 0.038
1.353GlnGlu: 1.353 ± 0.04
0.763GlnPhe: 0.763 ± 0.026
2.309GlnGly: 2.309 ± 0.047
0.732GlnHis: 0.732 ± 0.025
1.148GlnIle: 1.148 ± 0.038
0.548GlnLys: 0.548 ± 0.023
3.036GlnLeu: 3.036 ± 0.06
0.542GlnMet: 0.542 ± 0.025
0.447GlnAsn: 0.447 ± 0.017
1.675GlnPro: 1.675 ± 0.044
1.11GlnGln: 1.11 ± 0.035
2.535GlnArg: 2.535 ± 0.051
1.34GlnSer: 1.34 ± 0.035
1.544GlnThr: 1.544 ± 0.04
2.916GlnVal: 2.916 ± 0.051
0.494GlnTrp: 0.494 ± 0.022
0.395GlnTyr: 0.395 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
8.662ArgAla: 8.662 ± 0.099
0.516ArgCys: 0.516 ± 0.021
4.611ArgAsp: 4.611 ± 0.072
4.26ArgGlu: 4.26 ± 0.067
2.328ArgPhe: 2.328 ± 0.044
5.489ArgGly: 5.489 ± 0.071
1.86ArgHis: 1.86 ± 0.038
3.131ArgIle: 3.131 ± 0.053
1.617ArgLys: 1.617 ± 0.042
8.091ArgLeu: 8.091 ± 0.098
1.73ArgMet: 1.73 ± 0.037
1.317ArgAsn: 1.317 ± 0.039
4.153ArgPro: 4.153 ± 0.074
2.157ArgGln: 2.157 ± 0.045
7.1ArgArg: 7.1 ± 0.106
4.438ArgSer: 4.438 ± 0.07
4.422ArgThr: 4.422 ± 0.074
6.174ArgVal: 6.174 ± 0.082
1.294ArgTrp: 1.294 ± 0.038
1.351ArgTyr: 1.351 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.849SerAla: 6.849 ± 0.086
0.413SerCys: 0.413 ± 0.019
3.298SerAsp: 3.298 ± 0.058
2.809SerGlu: 2.809 ± 0.06
1.807SerPhe: 1.807 ± 0.044
5.831SerGly: 5.831 ± 0.087
1.144SerHis: 1.144 ± 0.034
1.996SerIle: 1.996 ± 0.043
1.12SerLys: 1.12 ± 0.035
5.473SerLeu: 5.473 ± 0.069
1.445SerMet: 1.445 ± 0.035
1.005SerAsn: 1.005 ± 0.03
3.246SerPro: 3.246 ± 0.068
1.528SerGln: 1.528 ± 0.036
3.987SerArg: 3.987 ± 0.064
3.59SerSer: 3.59 ± 0.063
3.766SerThr: 3.766 ± 0.07
4.717SerVal: 4.717 ± 0.074
0.963SerTrp: 0.963 ± 0.032
1.253SerTyr: 1.253 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.418ThrAla: 7.418 ± 0.095
0.529ThrCys: 0.529 ± 0.024
3.638ThrAsp: 3.638 ± 0.059
2.971ThrGlu: 2.971 ± 0.058
1.903ThrPhe: 1.903 ± 0.043
5.946ThrGly: 5.946 ± 0.074
1.264ThrHis: 1.264 ± 0.032
2.362ThrIle: 2.362 ± 0.054
1.277ThrLys: 1.277 ± 0.042
5.658ThrLeu: 5.658 ± 0.08
1.188ThrMet: 1.188 ± 0.037
1.125ThrAsn: 1.125 ± 0.036
3.938ThrPro: 3.938 ± 0.068
1.632ThrGln: 1.632 ± 0.042
4.037ThrArg: 4.037 ± 0.057
3.878ThrSer: 3.878 ± 0.064
4.337ThrThr: 4.337 ± 0.082
5.371ThrVal: 5.371 ± 0.084
1.04ThrTrp: 1.04 ± 0.035
1.455ThrTyr: 1.455 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
12.165ValAla: 12.165 ± 0.127
0.739ValCys: 0.739 ± 0.025
6.129ValAsp: 6.129 ± 0.076
5.69ValGlu: 5.69 ± 0.087
2.515ValPhe: 2.515 ± 0.053
7.665ValGly: 7.665 ± 0.093
1.966ValHis: 1.966 ± 0.042
3.498ValIle: 3.498 ± 0.063
1.84ValLys: 1.84 ± 0.05
9.889ValLeu: 9.889 ± 0.121
1.702ValMet: 1.702 ± 0.04
1.755ValAsn: 1.755 ± 0.04
4.763ValPro: 4.763 ± 0.062
2.127ValGln: 2.127 ± 0.038
6.682ValArg: 6.682 ± 0.091
5.202ValSer: 5.202 ± 0.081
5.979ValThr: 5.979 ± 0.088
10.276ValVal: 10.276 ± 0.109
1.17ValTrp: 1.17 ± 0.037
1.34ValTyr: 1.34 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.602TrpAla: 1.602 ± 0.047
0.163TrpCys: 0.163 ± 0.013
0.839TrpAsp: 0.839 ± 0.03
0.7TrpGlu: 0.7 ± 0.028
0.561TrpPhe: 0.561 ± 0.023
1.05TrpGly: 1.05 ± 0.034
0.363TrpHis: 0.363 ± 0.021
0.561TrpIle: 0.561 ± 0.022
0.318TrpLys: 0.318 ± 0.017
1.885TrpLeu: 1.885 ± 0.046
0.307TrpMet: 0.307 ± 0.015
0.322TrpAsn: 0.322 ± 0.018
0.761TrpPro: 0.761 ± 0.027
0.566TrpGln: 0.566 ± 0.023
1.339TrpArg: 1.339 ± 0.039
1.058TrpSer: 1.058 ± 0.032
0.902TrpThr: 0.902 ± 0.028
1.285TrpVal: 1.285 ± 0.034
0.397TrpTrp: 0.397 ± 0.025
0.234TrpTyr: 0.234 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.47TyrAla: 2.47 ± 0.054
0.167TyrCys: 0.167 ± 0.011
1.492TyrAsp: 1.492 ± 0.039
1.004TyrGlu: 1.004 ± 0.034
0.632TyrPhe: 0.632 ± 0.026
1.792TyrGly: 1.792 ± 0.042
0.308TyrHis: 0.308 ± 0.017
0.493TyrIle: 0.493 ± 0.021
0.375TyrLys: 0.375 ± 0.019
1.916TyrLeu: 1.916 ± 0.042
0.21TyrMet: 0.21 ± 0.015
0.358TyrAsn: 0.358 ± 0.02
0.927TyrPro: 0.927 ± 0.034
0.471TyrGln: 0.471 ± 0.019
1.315TyrArg: 1.315 ± 0.034
0.954TyrSer: 0.954 ± 0.029
0.997TyrThr: 0.997 ± 0.035
1.715TyrVal: 1.715 ± 0.042
0.279TyrTrp: 0.279 ± 0.018
0.43TyrTyr: 0.43 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3471 proteins (1077956 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski