Luteimonas sp. JM171
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2668 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B3W5G1|A0A1B3W5G1_9GAMM Uncharacterized protein OS=Luteimonas sp. JM171 OX=1896164 GN=BGP89_02155 PE=4 SV=1
AA1 pKa = 7.7 EE2 pKa = 5.0 GVDD5 pKa = 3.49 STAIGFFSHH14 pKa = 6.92 AEE16 pKa = 3.86 AAGSVALGVDD26 pKa = 3.77 SVADD30 pKa = 3.98 RR31 pKa = 11.84 EE32 pKa = 4.48 NTVSVGSAGNEE43 pKa = 3.49 RR44 pKa = 11.84 QIANVADD51 pKa = 3.79 GTEE54 pKa = 3.97 ATDD57 pKa = 4.38 AVNLGQLEE65 pKa = 4.34 AVTSDD70 pKa = 2.84 THH72 pKa = 6.84 YY73 pKa = 10.66 FAATGDD79 pKa = 3.88 GEE81 pKa = 4.66 AFATGLDD88 pKa = 3.68 ATASGSDD95 pKa = 3.18 AFAEE99 pKa = 4.48 GDD101 pKa = 3.66 YY102 pKa = 10.51 STATGSASWALGEE115 pKa = 4.21 GASAFGSGAQAWGQYY130 pKa = 7.99 ATASGFNAYY139 pKa = 10.26 AEE141 pKa = 4.78 GDD143 pKa = 3.65 STTAVGGWLYY153 pKa = 11.35 YY154 pKa = 10.23 EE155 pKa = 5.26 DD156 pKa = 5.04 PATGDD161 pKa = 3.57 VLLDD165 pKa = 3.34 ATTTAFEE172 pKa = 4.35 VGASAFGAGAQALGAFSTASGAAAEE197 pKa = 4.43 AGGLQATATGYY208 pKa = 10.69 SSSASGNYY216 pKa = 7.86 STASGGFSSAGDD228 pKa = 3.53 VGATALGYY236 pKa = 10.56 GADD239 pKa = 3.7 ASAGYY244 pKa = 8.37 ATAVGTAAWAQGVSSVAVGEE264 pKa = 4.13 YY265 pKa = 10.6 SEE267 pKa = 4.34 ATGAEE272 pKa = 4.32 SVAVGGTVFGFIPSRR287 pKa = 11.84 ASGAGAAAFGAGAWATGDD305 pKa = 3.39 EE306 pKa = 4.5 STALGWISSADD317 pKa = 3.41 GDD319 pKa = 3.97 YY320 pKa = 10.37 STAVGAASWAEE331 pKa = 3.95 AADD334 pKa = 3.93 STVVGYY340 pKa = 10.5 GGYY343 pKa = 10.77 ASAEE347 pKa = 3.91 NSVALGAGSVADD359 pKa = 4.16 RR360 pKa = 11.84 ADD362 pKa = 3.72 SVSVGSAGGEE372 pKa = 3.67 RR373 pKa = 11.84 QITNVAAGTEE383 pKa = 4.26 GTDD386 pKa = 3.31 AVNVEE391 pKa = 4.17 QLTDD395 pKa = 4.71 AIAAVDD401 pKa = 3.68 TEE403 pKa = 4.35 GNRR406 pKa = 11.84 FFDD409 pKa = 3.5 ASAGVDD415 pKa = 3.54 EE416 pKa = 5.5 AGVGASADD424 pKa = 3.68 GEE426 pKa = 4.48 YY427 pKa = 9.98 ATAGGEE433 pKa = 4.03 LASASGDD440 pKa = 3.32 LSVAMGALSDD450 pKa = 3.69 ASGWGATAVGVEE462 pKa = 4.61 SVASGEE468 pKa = 3.83 LAAAFGGAAEE478 pKa = 4.29 AFGDD482 pKa = 4.44 FSTALGGFSTAAGYY496 pKa = 11.09 NSTALGNFATATADD510 pKa = 3.3 NSVALGADD518 pKa = 3.38 SVADD522 pKa = 3.92 RR523 pKa = 11.84 DD524 pKa = 3.91 NTVSVGSEE532 pKa = 3.65 GSEE535 pKa = 3.59 RR536 pKa = 11.84 QITNVAAGTEE546 pKa = 4.23 GTDD549 pKa = 4.02 AVNLGQLEE557 pKa = 4.09 EE558 pKa = 4.05 ATAYY562 pKa = 9.64 NQYY565 pKa = 10.09 FRR567 pKa = 11.84 ATGEE571 pKa = 4.13 GEE573 pKa = 3.64 AWAVGEE579 pKa = 4.55 DD580 pKa = 3.34 ATAAGSNAWAGGDD593 pKa = 3.47 YY594 pKa = 9.68 STAVGSTAEE603 pKa = 3.82 AWMEE607 pKa = 3.89 GASAFGSGAFALEE620 pKa = 4.18 EE621 pKa = 3.95 NATALGFNTVAGGIDD636 pKa = 3.6 AVALGSYY643 pKa = 10.43 AEE645 pKa = 4.6 AYY647 pKa = 9.98 GEE649 pKa = 3.84 QSIALGSQAYY659 pKa = 9.45 AAEE662 pKa = 4.46 TGASAIGAGAVAEE675 pKa = 4.55 GAYY678 pKa = 8.92 STATGSEE685 pKa = 3.84 ASASGLQGTATGFRR699 pKa = 11.84 SEE701 pKa = 3.97 ASGDD705 pKa = 3.14 GSAAYY710 pKa = 9.75 GGYY713 pKa = 9.98 SVAQGFGASALGYY726 pKa = 9.49 GANALADD733 pKa = 3.78 GATAVGFAADD743 pKa = 3.56 ASGEE747 pKa = 4.16 SSVAVGEE754 pKa = 4.05 AAIASGFNSVAVGGASFFGLLPTEE778 pKa = 4.13 ATGDD782 pKa = 3.62 YY783 pKa = 8.57 ATAVGAGSWAPGANGTALGSLSMATGVDD811 pKa = 3.74 SLAVGADD818 pKa = 3.2 AFASAEE824 pKa = 4.13 GAVALGVGSEE834 pKa = 3.91 ADD836 pKa = 3.3 RR837 pKa = 11.84 DD838 pKa = 3.94 YY839 pKa = 11.58 SVSVGSAGNEE849 pKa = 3.59 RR850 pKa = 11.84 QITNVAAGTEE860 pKa = 4.1 ATDD863 pKa = 4.26 AVNLGQLEE871 pKa = 4.38 AVTGDD876 pKa = 3.34 THH878 pKa = 6.85 YY879 pKa = 10.57 FAASGEE885 pKa = 4.22 GQAWATGDD893 pKa = 3.75 DD894 pKa = 3.84 ATASGSDD901 pKa = 3.29 AFAEE905 pKa = 4.72 GDD907 pKa = 3.43 FSTATGATSFAVGQGASAFGSGAQAWGQYY936 pKa = 7.97 ATASGYY942 pKa = 10.39 NAVAEE947 pKa = 4.79 GDD949 pKa = 3.51 SSTAVGGWLYY959 pKa = 11.35 YY960 pKa = 10.19 EE961 pKa = 5.18 DD962 pKa = 4.72 PATGEE967 pKa = 4.15 VLLDD971 pKa = 3.41 QTTTALEE978 pKa = 4.32 AGASAFGAGAQALGAFSTATGAASTAEE1005 pKa = 4.17 GLQATATGYY1014 pKa = 11.04 SSTASGLASTANGGFSEE1031 pKa = 4.32 ATGEE1035 pKa = 3.95 FSTALGYY1042 pKa = 10.7 GAVASNTRR1050 pKa = 11.84 TTAVGISAEE1059 pKa = 3.98 ASGLNATAVGNTATASGSAGVAVGSSSSASGVNGTAVGGTAWATGEE1105 pKa = 4.2 DD1106 pKa = 3.93 ATALGQFAWATRR1118 pKa = 11.84 AGATALGQDD1127 pKa = 3.44 TFAYY1131 pKa = 10.36 ADD1133 pKa = 3.55 GATAVGASAWSNGVNSVALGSGSRR1157 pKa = 11.84 ASADD1161 pKa = 3.07 NVVSVGNGTGNSGFPATRR1179 pKa = 11.84 RR1180 pKa = 11.84 IVNVSDD1186 pKa = 4.34 GVDD1189 pKa = 3.64 DD1190 pKa = 4.17 NDD1192 pKa = 3.89 AVNVGQMGEE1201 pKa = 4.38 FVAEE1205 pKa = 4.38 ANRR1208 pKa = 11.84 HH1209 pKa = 4.12 FQATGEE1215 pKa = 3.99 ADD1217 pKa = 3.32 AYY1219 pKa = 10.92 ALGEE1223 pKa = 4.09 EE1224 pKa = 4.56 STAAGANAYY1233 pKa = 10.21 AEE1235 pKa = 4.65 ADD1237 pKa = 3.57 YY1238 pKa = 9.9 STAVGSASSAFEE1250 pKa = 4.27 LGASAFGSGALAGQQQATAVGFNASAAGSNSSAFGAGADD1289 pKa = 3.74 AYY1291 pKa = 10.31 GDD1293 pKa = 3.41 ASTAVGGQGEE1303 pKa = 4.37 AFDD1306 pKa = 3.92 EE1307 pKa = 4.79 FGWPIEE1313 pKa = 4.21 DD1314 pKa = 4.14 SEE1316 pKa = 4.66 GNPVLVAATAADD1328 pKa = 3.74 RR1329 pKa = 11.84 GTAVGAGAQSTGTGSASLGYY1349 pKa = 9.45 GARR1352 pKa = 11.84 AEE1354 pKa = 4.7 GEE1356 pKa = 3.81 FSLAGGHH1363 pKa = 6.53 KK1364 pKa = 9.93 AHH1366 pKa = 6.73 AQGGYY1371 pKa = 7.59 TVSLGDD1377 pKa = 3.44 NSRR1380 pKa = 11.84 ANGDD1384 pKa = 3.12 QSTATGSYY1392 pKa = 10.02 AWATEE1397 pKa = 4.14 SGSTALGSFAWATGEE1412 pKa = 3.8 EE1413 pKa = 4.19 ATAVGVDD1420 pKa = 3.65 SYY1422 pKa = 11.44 ATGRR1426 pKa = 11.84 RR1427 pKa = 11.84 SVSIGSGSWSPGVSSVAVGRR1447 pKa = 11.84 GAFGYY1452 pKa = 10.59 DD1453 pKa = 3.83 DD1454 pKa = 3.58 YY1455 pKa = 11.44 TVAVGHH1461 pKa = 5.8 QALGNDD1467 pKa = 2.75 IHH1469 pKa = 7.94 AIAVGTNSLANAEE1482 pKa = 4.15 SAIAIGAGATVAATAPNSVALGSGSVAEE1510 pKa = 4.39 RR1511 pKa = 11.84 EE1512 pKa = 4.19 RR1513 pKa = 11.84 TLSVGSVGNEE1523 pKa = 3.43 RR1524 pKa = 11.84 QITNVAAGTQDD1535 pKa = 3.01 TDD1537 pKa = 3.82 AVNLGQLNAVADD1549 pKa = 4.51 GVTALADD1556 pKa = 3.6 SAVVYY1561 pKa = 10.87 DD1562 pKa = 4.42 DD1563 pKa = 4.75 DD1564 pKa = 4.46 SRR1566 pKa = 11.84 GAITLAGEE1574 pKa = 4.2 EE1575 pKa = 4.52 GTLVTNLADD1584 pKa = 3.83 GEE1586 pKa = 4.51 VSAEE1590 pKa = 4.06 STDD1593 pKa = 3.51 AVTGSQLFEE1602 pKa = 4.01 TNEE1605 pKa = 3.87 RR1606 pKa = 11.84 VGVVEE1611 pKa = 5.01 GRR1613 pKa = 11.84 VDD1615 pKa = 3.76 DD1616 pKa = 4.35 LDD1618 pKa = 3.81 VRR1620 pKa = 11.84 IGDD1623 pKa = 3.91 VEE1625 pKa = 4.5 GMASNAISYY1634 pKa = 10.74 DD1635 pKa = 3.5 DD1636 pKa = 4.65 DD1637 pKa = 3.83 SRR1639 pKa = 11.84 AVATLDD1645 pKa = 3.64 GEE1647 pKa = 4.65 GGTRR1651 pKa = 11.84 LTNLAPGLIRR1661 pKa = 11.84 NGSSDD1666 pKa = 3.78 AVTGGQVFASMGTIATALGGGTVVDD1691 pKa = 3.97 ANGRR1695 pKa = 11.84 LIGTVYY1701 pKa = 10.46 SVQGNYY1707 pKa = 9.66 YY1708 pKa = 9.87 GNVGEE1713 pKa = 5.47 ALAALDD1719 pKa = 3.44 THH1721 pKa = 7.72 LEE1723 pKa = 4.21 GFGNRR1728 pKa = 11.84 LSGVEE1733 pKa = 4.13 AIVRR1737 pKa = 11.84 SAQGPGNGRR1746 pKa = 11.84 VAVGGAGRR1754 pKa = 11.84 AQLGAEE1760 pKa = 5.09 DD1761 pKa = 4.2 INAIAAGDD1769 pKa = 3.65 DD1770 pKa = 4.09 AYY1772 pKa = 11.05 AHH1774 pKa = 6.67 GPNDD1778 pKa = 3.21 IAIGGNAKK1786 pKa = 10.03 VNADD1790 pKa = 2.9 GSTAVGSNATITADD1804 pKa = 3.1 ATNAVAIGEE1813 pKa = 4.43 GTSVSAASGTAVGQGASVTAEE1834 pKa = 3.88 GAVALGQGSVADD1846 pKa = 4.17 RR1847 pKa = 11.84 ANTVSVGAEE1856 pKa = 3.68 GSEE1859 pKa = 3.79 RR1860 pKa = 11.84 QIVNVAAGTADD1871 pKa = 3.25 TDD1873 pKa = 3.81 AANVGQVNAAAAQSRR1888 pKa = 11.84 SYY1890 pKa = 10.24 TDD1892 pKa = 3.31 TVATQTLSSANAYY1905 pKa = 8.31 TDD1907 pKa = 3.12 QQFAVWNDD1915 pKa = 2.76 NFEE1918 pKa = 4.27 AFRR1921 pKa = 11.84 GDD1923 pKa = 3.47 VEE1925 pKa = 5.05 RR1926 pKa = 11.84 RR1927 pKa = 11.84 FHH1929 pKa = 7.47 DD1930 pKa = 3.02 VDD1932 pKa = 4.0 RR1933 pKa = 11.84 RR1934 pKa = 11.84 LDD1936 pKa = 3.47 RR1937 pKa = 11.84 QGAMSAAMLNMATSAAGIRR1956 pKa = 11.84 TQNRR1960 pKa = 11.84 IGVGVGFQSGEE1971 pKa = 4.01 SALSVGYY1978 pKa = 9.93 QRR1980 pKa = 11.84 AISEE1984 pKa = 4.23 RR1985 pKa = 11.84 ATITFGGAFSGDD1997 pKa = 3.53 EE1998 pKa = 4.05 SSVGIGAGFGWW2009 pKa = 3.8
Molecular weight: 193.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.643
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.834
Patrickios 1.837
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A1B3W7M5|A0A1B3W7M5_9GAMM Uncharacterized protein OS=Luteimonas sp. JM171 OX=1896164 GN=BGP89_06615 PE=4 SV=1
MM1 pKa = 7.29 HH2 pKa = 7.3 HH3 pKa = 7.24 PSASPEE9 pKa = 4.11 SRR11 pKa = 11.84 FLRR14 pKa = 11.84 QALAGAAFLGLVAVSTLSAARR35 pKa = 11.84 GGGPVGALALWLPGLPLLALAALALAGSRR64 pKa = 11.84 GHH66 pKa = 7.07 RR67 pKa = 11.84 DD68 pKa = 3.27 APASRR73 pKa = 11.84 PAPASSRR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 ARR84 pKa = 11.84 PAQARR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 APARR95 pKa = 11.84 PAHH98 pKa = 5.78 PRR100 pKa = 11.84 RR101 pKa = 11.84 RR102 pKa = 11.84 AAAA105 pKa = 3.68
Molecular weight: 10.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 10.891
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.144
Rodwell 12.164
Grimsley 12.705
Solomon 13.173
Lehninger 13.071
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 11.901
IPC_peptide 13.173
IPC2_peptide 12.164
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2668
0
2668
882712
41
2925
330.9
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.072 ± 0.076
0.744 ± 0.013
6.011 ± 0.038
6.122 ± 0.049
3.301 ± 0.027
9.132 ± 0.05
2.192 ± 0.025
4.068 ± 0.039
2.129 ± 0.042
10.601 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.315 ± 0.023
2.282 ± 0.032
5.527 ± 0.037
3.544 ± 0.027
8.205 ± 0.055
4.931 ± 0.045
4.553 ± 0.036
7.595 ± 0.043
1.54 ± 0.021
2.138 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here