Circovirus-like genome DCCV-1
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WH92|A0A190WH92_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DCCV-1 OX=1788437 PE=3 SV=1
MM1 pKa = 7.29 ATRR4 pKa = 11.84 HH5 pKa = 5.27 VVFTINNWTEE15 pKa = 4.2 EE16 pKa = 3.48 IWLEE20 pKa = 4.01 VTQFDD25 pKa = 3.56 WLYY28 pKa = 9.87 VVVGKK33 pKa = 10.39 EE34 pKa = 3.68 IGEE37 pKa = 4.35 SGTPHH42 pKa = 5.84 LQGYY46 pKa = 9.16 GCFHH50 pKa = 7.04 KK51 pKa = 10.34 KK52 pKa = 7.76 QRR54 pKa = 11.84 YY55 pKa = 5.61 ATLARR60 pKa = 11.84 KK61 pKa = 8.4 WKK63 pKa = 9.77 GHH65 pKa = 5.45 FEE67 pKa = 4.18 VSRR70 pKa = 11.84 GTPQQASDD78 pKa = 3.77 YY79 pKa = 8.91 CKK81 pKa = 10.51 KK82 pKa = 10.85 GGDD85 pKa = 3.66 YY86 pKa = 10.66 FEE88 pKa = 6.17 KK89 pKa = 11.22 GEE91 pKa = 4.1 LPEE94 pKa = 4.28 SQSAKK99 pKa = 10.3 GGEE102 pKa = 4.22 ATKK105 pKa = 10.76 KK106 pKa = 10.12 LYY108 pKa = 10.75 EE109 pKa = 4.46 DD110 pKa = 4.38 AFSLAQKK117 pKa = 10.99 GEE119 pKa = 4.05 ILSIPEE125 pKa = 3.75 PLRR128 pKa = 11.84 TRR130 pKa = 11.84 FYY132 pKa = 10.3 ATYY135 pKa = 10.42 KK136 pKa = 10.45 KK137 pKa = 10.1 VAKK140 pKa = 10.16 DD141 pKa = 3.45 YY142 pKa = 9.59 MPKK145 pKa = 10.17 PEE147 pKa = 4.72 SLSEE151 pKa = 4.49 LKK153 pKa = 10.83 NLWICGEE160 pKa = 4.08 AGVGKK165 pKa = 8.15 TVLADD170 pKa = 4.88 LILPDD175 pKa = 5.15 AYY177 pKa = 11.23 SKK179 pKa = 11.26 NCNKK183 pKa = 9.26 WWDD186 pKa = 4.07 GYY188 pKa = 9.2 QNEE191 pKa = 4.76 PGVIINDD198 pKa = 3.76 LGIEE202 pKa = 4.15 HH203 pKa = 7.15 KK204 pKa = 11.1 VLGHH208 pKa = 6.35 HH209 pKa = 6.51 LKK211 pKa = 10.68 LWGEE215 pKa = 3.95 HH216 pKa = 6.04 RR217 pKa = 11.84 GFIAEE222 pKa = 4.29 TKK224 pKa = 10.47 GGALHH229 pKa = 6.66 IRR231 pKa = 11.84 PEE233 pKa = 4.32 RR234 pKa = 11.84 IIITSQYY241 pKa = 10.55 EE242 pKa = 3.7 IGQVFEE248 pKa = 4.43 DD249 pKa = 3.95 WEE251 pKa = 4.28 TRR253 pKa = 11.84 DD254 pKa = 3.75 AIRR257 pKa = 11.84 RR258 pKa = 11.84 RR259 pKa = 11.84 YY260 pKa = 8.62 KK261 pKa = 8.31 QHH263 pKa = 6.85 KK264 pKa = 8.3 LTGAFTRR271 pKa = 11.84 EE272 pKa = 3.76 ALIEE276 pKa = 4.08 LASKK280 pKa = 10.49 LL281 pKa = 3.79
Molecular weight: 32.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.29
IPC2_protein 7.644
IPC_protein 7.614
Toseland 7.571
ProMoST 8.083
Dawson 8.331
Bjellqvist 8.478
Wikipedia 8.302
Rodwell 8.361
Grimsley 7.585
Solomon 8.478
Lehninger 8.478
Nozaki 8.624
DTASelect 8.331
Thurlkill 8.404
EMBOSS 8.551
Sillero 8.668
Patrickios 4.431
IPC_peptide 8.478
IPC2_peptide 7.38
IPC2.peptide.svr19 7.589
Protein with the highest isoelectric point:
>tr|A0A190WH92|A0A190WH92_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DCCV-1 OX=1788437 PE=3 SV=1
MM1 pKa = 7.29 ATRR4 pKa = 11.84 HH5 pKa = 5.27 VVFTINNWTEE15 pKa = 4.2 EE16 pKa = 3.48 IWLEE20 pKa = 4.01 VTQFDD25 pKa = 3.56 WLYY28 pKa = 9.87 VVVGKK33 pKa = 10.39 EE34 pKa = 3.68 IGEE37 pKa = 4.35 SGTPHH42 pKa = 5.84 LQGYY46 pKa = 9.16 GCFHH50 pKa = 7.04 KK51 pKa = 10.34 KK52 pKa = 7.76 QRR54 pKa = 11.84 YY55 pKa = 5.61 ATLARR60 pKa = 11.84 KK61 pKa = 8.4 WKK63 pKa = 9.77 GHH65 pKa = 5.45 FEE67 pKa = 4.18 VSRR70 pKa = 11.84 GTPQQASDD78 pKa = 3.77 YY79 pKa = 8.91 CKK81 pKa = 10.51 KK82 pKa = 10.85 GGDD85 pKa = 3.66 YY86 pKa = 10.66 FEE88 pKa = 6.17 KK89 pKa = 11.22 GEE91 pKa = 4.1 LPEE94 pKa = 4.28 SQSAKK99 pKa = 10.3 GGEE102 pKa = 4.22 ATKK105 pKa = 10.76 KK106 pKa = 10.12 LYY108 pKa = 10.75 EE109 pKa = 4.46 DD110 pKa = 4.38 AFSLAQKK117 pKa = 10.99 GEE119 pKa = 4.05 ILSIPEE125 pKa = 3.75 PLRR128 pKa = 11.84 TRR130 pKa = 11.84 FYY132 pKa = 10.3 ATYY135 pKa = 10.42 KK136 pKa = 10.45 KK137 pKa = 10.1 VAKK140 pKa = 10.16 DD141 pKa = 3.45 YY142 pKa = 9.59 MPKK145 pKa = 10.17 PEE147 pKa = 4.72 SLSEE151 pKa = 4.49 LKK153 pKa = 10.83 NLWICGEE160 pKa = 4.08 AGVGKK165 pKa = 8.15 TVLADD170 pKa = 4.88 LILPDD175 pKa = 5.15 AYY177 pKa = 11.23 SKK179 pKa = 11.26 NCNKK183 pKa = 9.26 WWDD186 pKa = 4.07 GYY188 pKa = 9.2 QNEE191 pKa = 4.76 PGVIINDD198 pKa = 3.76 LGIEE202 pKa = 4.15 HH203 pKa = 7.15 KK204 pKa = 11.1 VLGHH208 pKa = 6.35 HH209 pKa = 6.51 LKK211 pKa = 10.68 LWGEE215 pKa = 3.95 HH216 pKa = 6.04 RR217 pKa = 11.84 GFIAEE222 pKa = 4.29 TKK224 pKa = 10.47 GGALHH229 pKa = 6.66 IRR231 pKa = 11.84 PEE233 pKa = 4.32 RR234 pKa = 11.84 IIITSQYY241 pKa = 10.55 EE242 pKa = 3.7 IGQVFEE248 pKa = 4.43 DD249 pKa = 3.95 WEE251 pKa = 4.28 TRR253 pKa = 11.84 DD254 pKa = 3.75 AIRR257 pKa = 11.84 RR258 pKa = 11.84 RR259 pKa = 11.84 YY260 pKa = 8.62 KK261 pKa = 8.31 QHH263 pKa = 6.85 KK264 pKa = 8.3 LTGAFTRR271 pKa = 11.84 EE272 pKa = 3.76 ALIEE276 pKa = 4.08 LASKK280 pKa = 10.49 LL281 pKa = 3.79
Molecular weight: 32.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.29
IPC2_protein 7.644
IPC_protein 7.614
Toseland 7.571
ProMoST 8.083
Dawson 8.331
Bjellqvist 8.478
Wikipedia 8.302
Rodwell 8.361
Grimsley 7.585
Solomon 8.478
Lehninger 8.478
Nozaki 8.624
DTASelect 8.331
Thurlkill 8.404
EMBOSS 8.551
Sillero 8.668
Patrickios 4.431
IPC_peptide 8.478
IPC2_peptide 7.38
IPC2.peptide.svr19 7.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
281
281
281
281.0
32.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.762 ± 0.0
1.423 ± 0.0
3.915 ± 0.0
9.253 ± 0.0
3.559 ± 0.0
9.253 ± 0.0
3.559 ± 0.0
6.406 ± 0.0
9.253 ± 0.0
8.541 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.712 ± 0.0
2.491 ± 0.0
3.559 ± 0.0
3.915 ± 0.0
4.982 ± 0.0
4.27 ± 0.0
5.694 ± 0.0
4.626 ± 0.0
3.203 ± 0.0
4.626 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here