Sanxia atyid shrimp virus 1
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KK18|A0A1L3KK18_9VIRU Uncharacterized protein OS=Sanxia atyid shrimp virus 1 OX=1923355 PE=4 SV=1
MM1 pKa = 7.43 NFLTASKK8 pKa = 7.36 TTLALIWLRR17 pKa = 11.84 IAPMAIFWIVSSVVRR32 pKa = 11.84 LSVTTVCPNQLLNRR46 pKa = 11.84 LNTLNSLFPVAISVVLHH63 pKa = 5.14 VLRR66 pKa = 11.84 FFLTLLSHH74 pKa = 5.98 VNILILMLVIRR85 pKa = 11.84 GLEE88 pKa = 3.85 FSSLIFSLITSCPLSLLLYY107 pKa = 10.14 YY108 pKa = 11.1 LLLFLLAVSPAFLLAAAVANKK129 pKa = 10.01 LFSPSSATTCVFMALLPLVTSDD151 pKa = 4.86 ALFLSSSSVHH161 pKa = 6.42 FNDD164 pKa = 2.98 ITQGSISPSPSVPSGQWFLTSPLTMLNPEE193 pKa = 4.25 CSYY196 pKa = 11.09 ILSTSRR202 pKa = 11.84 IWPSEE207 pKa = 3.92 CEE209 pKa = 4.2 LPLSCGDD216 pKa = 3.61 MLEE219 pKa = 4.2 KK220 pKa = 10.56 QSLGGAFFSEE230 pKa = 4.06 TDD232 pKa = 2.99 LCFRR236 pKa = 11.84 FFHH239 pKa = 7.54 DD240 pKa = 3.22 YY241 pKa = 9.2 HH242 pKa = 6.51 TFQSYY247 pKa = 8.83 IVYY250 pKa = 10.23 GILEE254 pKa = 4.36 GTLNTSSYY262 pKa = 11.1 QIDD265 pKa = 3.94 FHH267 pKa = 9.37 SFDD270 pKa = 3.8 PPIWLHH276 pKa = 5.9 SPSARR281 pKa = 11.84 RR282 pKa = 11.84 SGWIHH287 pKa = 7.07 LDD289 pKa = 3.01 AAHH292 pKa = 6.56 YY293 pKa = 8.93 FLHH296 pKa = 6.86 YY297 pKa = 10.78 DD298 pKa = 3.52 PLKK301 pKa = 11.08 NKK303 pKa = 9.04 YY304 pKa = 9.6 AWDD307 pKa = 3.51 SSLFNTKK314 pKa = 10.09 RR315 pKa = 11.84 EE316 pKa = 3.98 CHH318 pKa = 5.47 MVNTSAVFFEE328 pKa = 5.07 APYY331 pKa = 10.35 IDD333 pKa = 4.76 YY334 pKa = 7.73 NTSSLLLHH342 pKa = 6.9 DD343 pKa = 6.43 LIFQDD348 pKa = 4.51 YY349 pKa = 9.44 LSSGIPLNYY358 pKa = 9.03 TLHH361 pKa = 6.44 PKK363 pKa = 8.25 KK364 pKa = 9.05 TYY366 pKa = 10.96 AFGTEE371 pKa = 4.07 LVLSFVPNSYY381 pKa = 10.6 FSFSVLGNGFISSDD395 pKa = 3.75 SFTHH399 pKa = 6.77 LLDD402 pKa = 3.8 NGTYY406 pKa = 8.35 ITIEE410 pKa = 4.14 SDD412 pKa = 3.57 PEE414 pKa = 4.02 TLSISLYY421 pKa = 10.22 PLDD424 pKa = 4.32 FNPFVEE430 pKa = 3.82 IFEE433 pKa = 4.51 YY434 pKa = 10.31 KK435 pKa = 9.63 GHH437 pKa = 6.34 SFSFMCTSTCAVEE450 pKa = 4.18 FLHH453 pKa = 5.67 YY454 pKa = 10.03 TNILSWLNLTSTSIKK469 pKa = 8.51 NTKK472 pKa = 7.72 EE473 pKa = 3.46 RR474 pKa = 11.84 CVRR477 pKa = 11.84 SISKK481 pKa = 10.07 VSYY484 pKa = 8.54 TNFVTGIVYY493 pKa = 10.38 RR494 pKa = 11.84 SISTFEE500 pKa = 3.84 HH501 pKa = 5.49 AVIRR505 pKa = 11.84 LFRR508 pKa = 11.84 LFVDD512 pKa = 5.47 AISTAFQQLLTYY524 pKa = 8.93 VLSMEE529 pKa = 4.21 RR530 pKa = 11.84 QFKK533 pKa = 10.32 IIEE536 pKa = 4.09 FGIILALCTAHH547 pKa = 6.64 FNNISTALLLTLSIWYY563 pKa = 7.65 VAPFNTQQ570 pKa = 2.34
Molecular weight: 64.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.578
IPC2_protein 5.728
IPC_protein 5.804
Toseland 6.237
ProMoST 6.186
Dawson 6.122
Bjellqvist 6.122
Wikipedia 6.148
Rodwell 6.122
Grimsley 6.376
Solomon 6.122
Lehninger 6.122
Nozaki 6.415
DTASelect 6.605
Thurlkill 6.634
EMBOSS 6.59
Sillero 6.517
Patrickios 0.896
IPC_peptide 6.148
IPC2_peptide 6.517
IPC2.peptide.svr19 6.397
Protein with the highest isoelectric point:
>tr|A0A1L3KKA1|A0A1L3KKA1_9VIRU Helicase OS=Sanxia atyid shrimp virus 1 OX=1923355 PE=4 SV=1
MM1 pKa = 7.95 ADD3 pKa = 3.05 TAKK6 pKa = 10.47 RR7 pKa = 11.84 PRR9 pKa = 11.84 FFTRR13 pKa = 11.84 PPPMMPRR20 pKa = 11.84 GPPPQARR27 pKa = 11.84 KK28 pKa = 9.09 SARR31 pKa = 11.84 SRR33 pKa = 11.84 KK34 pKa = 9.22 NGQSSDD40 pKa = 3.57 FFEE43 pKa = 4.91 GVLDD47 pKa = 3.81 ALRR50 pKa = 11.84 RR51 pKa = 11.84 TSSQPDD57 pKa = 3.88 VLCLLALALLVAYY70 pKa = 9.56 DD71 pKa = 3.31 ITHH74 pKa = 7.24 IDD76 pKa = 2.94 RR77 pKa = 11.84 LAAFFSARR85 pKa = 11.84 TSTTQLGSWISANSQVIAGLVILLPGLYY113 pKa = 9.85 KK114 pKa = 10.68 FPVKK118 pKa = 10.56 SRR120 pKa = 11.84 FIAGIVTFVTVLALPSQSSYY140 pKa = 11.34 VYY142 pKa = 9.62 FAVSLGMTVFWQVRR156 pKa = 11.84 QPGIRR161 pKa = 11.84 LVLFSALLLYY171 pKa = 10.67 LYY173 pKa = 9.84 VFNGAALGISPTTSATPPPVTTTTASTTTRR203 pKa = 11.84 PPAGRR208 pKa = 3.68
Molecular weight: 22.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 10.145
IPC_protein 11.184
Toseland 11.155
ProMoST 11.564
Dawson 11.213
Bjellqvist 11.111
Wikipedia 11.594
Rodwell 11.155
Grimsley 11.272
Solomon 11.55
Lehninger 11.477
Nozaki 11.14
DTASelect 11.111
Thurlkill 11.155
EMBOSS 11.608
Sillero 11.184
Patrickios 10.862
IPC_peptide 11.55
IPC2_peptide 10.467
IPC2.peptide.svr19 8.742
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3699
128
2629
739.8
82.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.921 ± 0.686
2.379 ± 0.685
6.164 ± 1.489
3.839 ± 0.629
5.758 ± 0.89
4.38 ± 0.445
2.703 ± 0.335
6.11 ± 0.443
4.866 ± 1.221
10.192 ± 1.614
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.974 ± 0.265
4.028 ± 0.435
5.569 ± 0.565
2.595 ± 0.494
4.677 ± 0.702
9.651 ± 1.041
5.434 ± 1.214
7.651 ± 0.728
1.027 ± 0.237
4.082 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here