Anaerosacchriphilus polymeriproducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerosacchariphilus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371ASX5|A0A371ASX5_9FIRM Heme chaperone HemW OS=Anaerosacchriphilus polymeriproducens OX=1812858 GN=DWV06_14340 PE=3 SV=1
MM1 pKa = 7.61TNYY4 pKa = 10.26QSIFQTGTLEE14 pKa = 5.06SGFTYY19 pKa = 10.36NAPQVVDD26 pKa = 3.28NTSYY30 pKa = 11.53NFVGTKK36 pKa = 9.82SYY38 pKa = 11.22SGTTGFTDD46 pKa = 3.17VATLIGATYY55 pKa = 10.82DD56 pKa = 3.32SGTGKK61 pKa = 7.99MTQGLTKK68 pKa = 10.69AEE70 pKa = 3.97ISSMLFTKK78 pKa = 10.78SMGAYY83 pKa = 9.61NLNDD87 pKa = 3.49AVAWTYY93 pKa = 11.54DD94 pKa = 3.39YY95 pKa = 10.3GTVGDD100 pKa = 4.73GSDD103 pKa = 3.21ATVTDD108 pKa = 3.84GTTTVPVSILTSCGITGTSALADD131 pKa = 3.99GDD133 pKa = 4.34PVTGLTWEE141 pKa = 4.06MTNAEE146 pKa = 4.62VVTALQAAEE155 pKa = 4.33TNPDD159 pKa = 3.45IAWSYY164 pKa = 8.92STGSGYY170 pKa = 10.87VATHH174 pKa = 5.51TTTLGNSDD182 pKa = 4.9LNTGYY187 pKa = 10.89YY188 pKa = 9.59YY189 pKa = 10.96DD190 pKa = 3.7SAQGQFEE197 pKa = 4.24QGGTIVTAAEE207 pKa = 4.46AYY209 pKa = 9.04GIPADD214 pKa = 3.69TDD216 pKa = 3.57AVKK219 pKa = 9.37NTFTQNADD227 pKa = 3.03GSITLSTTTKK237 pKa = 8.17TKK239 pKa = 10.79DD240 pKa = 3.24YY241 pKa = 10.08IVQFNTSTGAFVSVNGEE258 pKa = 3.92DD259 pKa = 4.91DD260 pKa = 3.9VNLDD264 pKa = 3.76FAAGYY269 pKa = 11.46GNFKK273 pKa = 10.24DD274 pKa = 3.68INMDD278 pKa = 4.22FKK280 pKa = 10.93GTTMVDD286 pKa = 3.28NNGSSTLVMEE296 pKa = 4.84TGDD299 pKa = 3.81ADD301 pKa = 3.98GVGAGKK307 pKa = 10.68KK308 pKa = 9.21LGQMTGVSIQNNGMIYY324 pKa = 8.89GTYY327 pKa = 10.76DD328 pKa = 3.03NGNTKK333 pKa = 10.53LLGQIATASFANASGLLKK351 pKa = 10.55EE352 pKa = 4.55GEE354 pKa = 4.27NLYY357 pKa = 10.91SATLNSGEE365 pKa = 4.34FDD367 pKa = 4.86GIGQDD372 pKa = 3.46VTADD376 pKa = 3.65GGSMSSGVLEE386 pKa = 4.19MSNVDD391 pKa = 3.73LSAEE395 pKa = 4.03FTEE398 pKa = 5.13MITTQRR404 pKa = 11.84GFQANSRR411 pKa = 11.84IITTSDD417 pKa = 2.83SMLEE421 pKa = 3.66EE422 pKa = 5.03LINLKK427 pKa = 10.35RR428 pKa = 3.63

Molecular weight: 44.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371AVF4|A0A371AVF4_9FIRM Uncharacterized protein OS=Anaerosacchriphilus polymeriproducens OX=1812858 GN=DWV06_09000 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3531

0

3531

1128028

24

4564

319.5

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.939 ± 0.044

1.355 ± 0.015

5.216 ± 0.03

7.578 ± 0.052

4.443 ± 0.037

6.448 ± 0.036

1.569 ± 0.016

9.17 ± 0.043

8.37 ± 0.034

8.986 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.019

5.512 ± 0.037

2.833 ± 0.023

3.248 ± 0.027

3.493 ± 0.027

6.144 ± 0.029

5.184 ± 0.034

6.519 ± 0.032

0.826 ± 0.014

4.316 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski