Mycoplasma caviae
Average proteome isoelectric point is 7.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1008 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P8L7L7|A0A3P8L7L7_9MOLU 30S ribosomal protein S10 OS=Mycoplasma caviae OX=55603 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.02 SWKK4 pKa = 9.37 TKK6 pKa = 10.08 ILVVSDD12 pKa = 2.73 IHH14 pKa = 7.83 GDD16 pKa = 3.61 LSTLSAIVSVEE27 pKa = 3.8 MPDD30 pKa = 4.06 YY31 pKa = 10.76 IISCGDD37 pKa = 3.17 NLLPKK42 pKa = 10.46 SFMQEE47 pKa = 3.48 MFSYY51 pKa = 10.86 YY52 pKa = 10.42 VDD54 pKa = 3.76 GNNDD58 pKa = 3.28 EE59 pKa = 4.82 LDD61 pKa = 3.46 EE62 pKa = 4.38 EE63 pKa = 5.2 FYY65 pKa = 11.5 SRR67 pKa = 11.84 IPSARR72 pKa = 11.84 FEE74 pKa = 4.11 IDD76 pKa = 3.2 GLCFYY81 pKa = 10.94 VEE83 pKa = 4.49 HH84 pKa = 6.32 GHH86 pKa = 6.64 NLGNYY91 pKa = 7.89 EE92 pKa = 5.06 LLRR95 pKa = 11.84 DD96 pKa = 3.9 PKK98 pKa = 10.59 KK99 pKa = 11.04 VYY101 pKa = 10.93 LDD103 pKa = 3.99 FDD105 pKa = 3.77 TLAIKK110 pKa = 9.4 TYY112 pKa = 10.89
Molecular weight: 12.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.114
IPC2_protein 4.431
IPC_protein 4.342
Toseland 4.164
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.228
Rodwell 4.177
Grimsley 4.075
Solomon 4.304
Lehninger 4.266
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.469
Patrickios 2.003
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.371
Protein with the highest isoelectric point:
>tr|A0A3P8MEG6|A0A3P8MEG6_9MOLU Protein of uncharacterized function (DUF402) OS=Mycoplasma caviae OX=55603 GN=NCTC10126_00175 PE=4 SV=1
MM1 pKa = 7.52 ARR3 pKa = 11.84 ILNIEE8 pKa = 3.76 IPNNKK13 pKa = 9.0 RR14 pKa = 11.84 VVISLTYY21 pKa = 9.52 IYY23 pKa = 10.74 GIGRR27 pKa = 11.84 TRR29 pKa = 11.84 AQEE32 pKa = 3.49 ICAKK36 pKa = 10.7 AKK38 pKa = 10.17 INEE41 pKa = 4.01 NLRR44 pKa = 11.84 VKK46 pKa = 10.53 DD47 pKa = 3.86 LSEE50 pKa = 4.18 EE51 pKa = 3.84 QLSAIRR57 pKa = 11.84 EE58 pKa = 4.1 VAKK61 pKa = 10.51 EE62 pKa = 3.8 YY63 pKa = 8.2 TTEE66 pKa = 4.02 GDD68 pKa = 3.5 LRR70 pKa = 11.84 RR71 pKa = 11.84 EE72 pKa = 3.51 TSLNIKK78 pKa = 10.06 RR79 pKa = 11.84 LMEE82 pKa = 4.08 IKK84 pKa = 9.93 CYY86 pKa = 10.25 RR87 pKa = 11.84 GIRR90 pKa = 11.84 HH91 pKa = 6.31 RR92 pKa = 11.84 KK93 pKa = 7.28 GLPVRR98 pKa = 11.84 GQCTQKK104 pKa = 10.57 NARR107 pKa = 11.84 TRR109 pKa = 11.84 KK110 pKa = 9.32 GPRR113 pKa = 11.84 KK114 pKa = 7.39 TVAGKK119 pKa = 10.2 KK120 pKa = 10.08 SKK122 pKa = 10.84
Molecular weight: 14.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.692
IPC_protein 10.175
Toseland 10.76
ProMoST 10.379
Dawson 10.833
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 11.213
Grimsley 10.877
Solomon 10.921
Lehninger 10.906
Nozaki 10.745
DTASelect 10.482
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.76
Patrickios 10.935
IPC_peptide 10.935
IPC2_peptide 9.355
IPC2.peptide.svr19 8.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1008
0
1008
344285
30
3316
341.6
39.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.045 ± 0.085
0.686 ± 0.022
5.524 ± 0.055
6.915 ± 0.075
5.38 ± 0.058
4.564 ± 0.073
1.473 ± 0.024
9.189 ± 0.083
10.877 ± 0.075
9.356 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.866 ± 0.03
7.796 ± 0.091
2.484 ± 0.044
3.022 ± 0.036
3.134 ± 0.045
6.901 ± 0.059
4.955 ± 0.053
5.433 ± 0.046
0.981 ± 0.022
4.417 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here