Mount Mabu Lophuromys virus 2
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GJB4|A0A2P1GJB4_9MONO Putative TM protein OS=Mount Mabu Lophuromys virus 2 OX=2116560 GN=TM PE=4 SV=1
MM1 pKa = 7.42 SRR3 pKa = 11.84 LNSVLEE9 pKa = 3.93 EE10 pKa = 3.68 FRR12 pKa = 11.84 EE13 pKa = 4.56 FKK15 pKa = 10.95 NNPPKK20 pKa = 10.51 RR21 pKa = 11.84 GLISSALHH29 pKa = 5.62 GLKK32 pKa = 10.19 KK33 pKa = 10.57 SVIIPVPTFVDD44 pKa = 5.5 PIQRR48 pKa = 11.84 WYY50 pKa = 11.26 FMVFMLEE57 pKa = 4.23 LAWSNVASGSIMTGALLSLLSLFAEE82 pKa = 4.63 NPGAMVRR89 pKa = 11.84 SLLNDD94 pKa = 3.86 PDD96 pKa = 5.16 LDD98 pKa = 3.89 VQIAEE103 pKa = 4.23 VSRR106 pKa = 11.84 ASKK109 pKa = 11.13 DD110 pKa = 3.6 EE111 pKa = 3.99 IKK113 pKa = 10.8 LATRR117 pKa = 11.84 GKK119 pKa = 10.53 NMEE122 pKa = 4.66 DD123 pKa = 3.63 YY124 pKa = 10.12 EE125 pKa = 5.01 KK126 pKa = 10.99 EE127 pKa = 4.13 LIRR130 pKa = 11.84 IAGYY134 pKa = 10.52 GPYY137 pKa = 9.71 QGTKK141 pKa = 8.49 TDD143 pKa = 3.89 PFPFVKK149 pKa = 10.01 KK150 pKa = 10.42 DD151 pKa = 3.31 QDD153 pKa = 3.34 KK154 pKa = 10.56 MITKK158 pKa = 7.73 TTEE161 pKa = 3.77 DD162 pKa = 3.41 LQLAVQTVTIQIWILLTKK180 pKa = 10.49 AVTATDD186 pKa = 3.53 TAKK189 pKa = 10.56 EE190 pKa = 4.22 SEE192 pKa = 3.71 NRR194 pKa = 11.84 RR195 pKa = 11.84 WVKK198 pKa = 10.28 YY199 pKa = 8.73 LQQRR203 pKa = 11.84 RR204 pKa = 11.84 ADD206 pKa = 3.9 RR207 pKa = 11.84 DD208 pKa = 3.7 YY209 pKa = 11.53 KK210 pKa = 11.38 LEE212 pKa = 4.21 DD213 pKa = 2.83 TWLNLARR220 pKa = 11.84 ARR222 pKa = 11.84 IAEE225 pKa = 4.77 DD226 pKa = 2.91 ISVRR230 pKa = 11.84 RR231 pKa = 11.84 YY232 pKa = 7.94 MVEE235 pKa = 4.0 ILIEE239 pKa = 4.23 TNKK242 pKa = 9.28 MAGVKK247 pKa = 8.91 TRR249 pKa = 11.84 IVEE252 pKa = 4.32 MISDD256 pKa = 3.73 IGNYY260 pKa = 9.35 ISEE263 pKa = 4.29 AGMAGFFLTIKK274 pKa = 10.67 YY275 pKa = 10.24 GIEE278 pKa = 3.86 TKK280 pKa = 10.96 YY281 pKa = 10.24 PALALNEE288 pKa = 4.12 LQADD292 pKa = 4.15 LSIILSLMKK301 pKa = 10.07 TYY303 pKa = 10.66 TSLGEE308 pKa = 3.87 KK309 pKa = 9.73 APYY312 pKa = 9.25 MVILEE317 pKa = 4.66 DD318 pKa = 5.19 AIQTKK323 pKa = 9.64 FSPGNYY329 pKa = 8.42 PLLWSYY335 pKa = 12.02 AMGVGSVLDD344 pKa = 3.37 RR345 pKa = 11.84 AVNNLNYY352 pKa = 8.54 TRR354 pKa = 11.84 PYY356 pKa = 10.0 LEE358 pKa = 4.81 HH359 pKa = 7.66 SFFQLGEE366 pKa = 4.21 TMVEE370 pKa = 4.02 KK371 pKa = 10.78 MEE373 pKa = 4.12 GTVNNRR379 pKa = 11.84 IAAEE383 pKa = 4.19 LDD385 pKa = 3.43 LSPEE389 pKa = 4.09 QVQSIRR395 pKa = 11.84 SIVKK399 pKa = 10.0 LDD401 pKa = 3.47 QATSGPRR408 pKa = 11.84 VSGPQQKK415 pKa = 8.89 TSGPRR420 pKa = 11.84 PFEE423 pKa = 4.37 LYY425 pKa = 10.27 NAGDD429 pKa = 4.58 IIPEE433 pKa = 3.98 IEE435 pKa = 4.37 EE436 pKa = 4.15 EE437 pKa = 4.93 VSDD440 pKa = 4.03 EE441 pKa = 4.37 DD442 pKa = 5.32 KK443 pKa = 11.28 KK444 pKa = 10.97 DD445 pKa = 3.36 TYY447 pKa = 11.16 DD448 pKa = 3.18 VSKK451 pKa = 10.96 VVPVPNANSIFYY463 pKa = 10.93 DD464 pKa = 4.06 KK465 pKa = 11.22 DD466 pKa = 3.4 NQKK469 pKa = 11.06 DD470 pKa = 3.62 KK471 pKa = 11.47 RR472 pKa = 11.84 NLPKK476 pKa = 10.02 PPKK479 pKa = 9.28 PSRR482 pKa = 11.84 SGKK485 pKa = 9.64 KK486 pKa = 9.6 VNNLKK491 pKa = 10.64 EE492 pKa = 4.29 SLNDD496 pKa = 3.6 ALEE499 pKa = 4.37 DD500 pKa = 4.0 FVRR503 pKa = 11.84 DD504 pKa = 3.16 EE505 pKa = 6.06 SMTQKK510 pKa = 10.62 LNKK513 pKa = 9.57 YY514 pKa = 10.38 DD515 pKa = 4.2 EE516 pKa = 5.63 DD517 pKa = 4.08 DD518 pKa = 3.46 VSYY521 pKa = 10.04 STDD524 pKa = 3.21 QSPATMSDD532 pKa = 3.09 LDD534 pKa = 3.81 ALRR537 pKa = 11.84 SS538 pKa = 3.64
Molecular weight: 60.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.185
IPC2_protein 5.245
IPC_protein 5.181
Toseland 5.054
ProMoST 5.283
Dawson 5.13
Bjellqvist 5.27
Wikipedia 4.991
Rodwell 5.029
Grimsley 4.965
Solomon 5.118
Lehninger 5.08
Nozaki 5.232
DTASelect 5.385
Thurlkill 5.041
EMBOSS 5.003
Sillero 5.296
Patrickios 4.406
IPC_peptide 5.13
IPC2_peptide 5.296
IPC2.peptide.svr19 5.35
Protein with the highest isoelectric point:
>tr|A0A2P1GJ92|A0A2P1GJ92_9MONO RNA-directed RNA polymerase L OS=Mount Mabu Lophuromys virus 2 OX=2116560 GN=L PE=3 SV=1
MM1 pKa = 7.51 EE2 pKa = 5.31 SALSTLSSRR11 pKa = 11.84 IRR13 pKa = 11.84 RR14 pKa = 11.84 TFRR17 pKa = 11.84 RR18 pKa = 11.84 HH19 pKa = 4.34 TGEE22 pKa = 3.99 VQSINHH28 pKa = 5.52 QPRR31 pKa = 11.84 IEE33 pKa = 3.99 QRR35 pKa = 11.84 RR36 pKa = 11.84 GRR38 pKa = 11.84 PSQRR42 pKa = 11.84 VRR44 pKa = 11.84 VEE46 pKa = 4.02 DD47 pKa = 3.88 LSVNPGEE54 pKa = 4.01 WEE56 pKa = 3.79 AAKK59 pKa = 8.75 EE60 pKa = 4.31 TRR62 pKa = 11.84 KK63 pKa = 8.67 RR64 pKa = 11.84 TAAEE68 pKa = 3.72 VLEE71 pKa = 5.52 LIDD74 pKa = 4.0 HH75 pKa = 6.76 LEE77 pKa = 4.25 SKK79 pKa = 9.15 GTEE82 pKa = 3.79 VTAPRR87 pKa = 11.84 TPRR90 pKa = 11.84 EE91 pKa = 3.67 QEE93 pKa = 4.08 MITKK97 pKa = 10.49 LGMLKK102 pKa = 9.94 ILLNLVKK109 pKa = 9.34 EE110 pKa = 4.5 TGVIPDD116 pKa = 4.38 PNKK119 pKa = 10.42 QSIQQSGLLTRR130 pKa = 11.84 VEE132 pKa = 4.14 MEE134 pKa = 4.47 NLGEE138 pKa = 4.41 MIPALKK144 pKa = 10.5 SILKK148 pKa = 9.84 NN149 pKa = 3.45
Molecular weight: 16.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.973
IPC2_protein 8.975
IPC_protein 9.048
Toseland 10.292
ProMoST 9.692
Dawson 10.335
Bjellqvist 9.882
Wikipedia 10.423
Rodwell 10.774
Grimsley 10.335
Solomon 10.409
Lehninger 10.409
Nozaki 10.248
DTASelect 9.882
Thurlkill 10.248
EMBOSS 10.657
Sillero 10.248
Patrickios 10.57
IPC_peptide 10.423
IPC2_peptide 8.156
IPC2.peptide.svr19 8.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5346
149
2188
668.3
75.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.863 ± 0.361
1.553 ± 0.373
5.724 ± 0.414
5.556 ± 0.499
3.236 ± 0.351
5.743 ± 0.709
2.132 ± 0.411
7.838 ± 0.535
6.023 ± 0.675
9.39 ± 0.814
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.768 ± 0.186
6.192 ± 0.389
3.984 ± 0.297
3.498 ± 0.287
5.051 ± 0.336
8.474 ± 0.395
6.921 ± 1.003
5.855 ± 0.529
1.066 ± 0.141
4.134 ± 0.56
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here