Paenibacillus sp. JC52

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6695 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559KE67|A0A559KE67_9BACL Prepilin-type N-terminal cleavage/methylation domain-containing protein OS=Paenibacillus sp. JC52 OX=2163881 GN=FPZ49_08420 PE=4 SV=1
MM1 pKa = 8.27DD2 pKa = 5.94DD3 pKa = 4.8FYY5 pKa = 11.43LTGDD9 pKa = 3.95TPAAMAGDD17 pKa = 4.02MEE19 pKa = 4.54YY20 pKa = 10.89HH21 pKa = 6.82FDD23 pKa = 3.82IKK25 pKa = 11.02LL26 pKa = 3.39

Molecular weight: 2.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559KF32|A0A559KF32_9BACL MBL fold metallo-hydrolase OS=Paenibacillus sp. JC52 OX=2163881 GN=FPZ49_06355 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.81PNVRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84SKK14 pKa = 9.51VHH16 pKa = 5.89GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6695

0

6695

2134665

25

5318

318.8

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.302 ± 0.04

0.838 ± 0.009

5.007 ± 0.023

6.381 ± 0.039

4.182 ± 0.024

7.361 ± 0.029

2.171 ± 0.016

6.505 ± 0.03

5.475 ± 0.026

10.087 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.844 ± 0.016

3.739 ± 0.025

4.222 ± 0.019

4.077 ± 0.021

4.976 ± 0.028

6.338 ± 0.027

5.517 ± 0.032

7.155 ± 0.024

1.324 ± 0.012

3.5 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski