Fusarium redolens polymycovirus 1
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A513ZVE3|A0A513ZVE3_9VIRU Uncharacterized protein OS=Fusarium redolens polymycovirus 1 OX=2546034 PE=4 SV=1
MM1 pKa = 7.34 SGKK4 pKa = 10.33 SFVIQTEE11 pKa = 4.31 CEE13 pKa = 4.19 GVGRR17 pKa = 11.84 RR18 pKa = 11.84 GLVSASVMEE27 pKa = 4.68 FYY29 pKa = 10.84 FIIGVVEE36 pKa = 4.41 DD37 pKa = 3.71 VCQEE41 pKa = 3.87 VATRR45 pKa = 11.84 LACLEE50 pKa = 4.31 AEE52 pKa = 4.59 FKK54 pKa = 11.01 EE55 pKa = 4.55 SGAVAACPGGWACAGVRR72 pKa = 11.84 LVVDD76 pKa = 4.12 PASVQVRR83 pKa = 11.84 AVSTDD88 pKa = 2.93 VSLDD92 pKa = 3.04 GSLRR96 pKa = 11.84 TLRR99 pKa = 11.84 CKK101 pKa = 10.55 ARR103 pKa = 11.84 AIVAIAPSDD112 pKa = 3.38 QTDD115 pKa = 3.83 RR116 pKa = 11.84 IGPIFDD122 pKa = 4.0 AAVQVAQRR130 pKa = 11.84 EE131 pKa = 4.33 LGVLGVVGLARR142 pKa = 11.84 LAVSWHH148 pKa = 5.16 GMAEE152 pKa = 4.02 QVRR155 pKa = 11.84 VLKK158 pKa = 10.53 PGEE161 pKa = 4.2 GSEE164 pKa = 4.55 KK165 pKa = 10.37 DD166 pKa = 3.31 TT167 pKa = 4.86
Molecular weight: 17.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.165
IPC2_protein 5.308
IPC_protein 5.143
Toseland 5.029
ProMoST 5.258
Dawson 5.105
Bjellqvist 5.245
Wikipedia 4.952
Rodwell 5.016
Grimsley 4.952
Solomon 5.105
Lehninger 5.054
Nozaki 5.219
DTASelect 5.334
Thurlkill 5.041
EMBOSS 4.991
Sillero 5.296
Patrickios 4.24
IPC_peptide 5.105
IPC2_peptide 5.283
IPC2.peptide.svr19 5.342
Protein with the highest isoelectric point:
>tr|A0A513ZVE6|A0A513ZVE6_9VIRU Putative capsid protein OS=Fusarium redolens polymycovirus 1 OX=2546034 PE=4 SV=1
MM1 pKa = 7.8 ASTTSSVQEE10 pKa = 3.77 WAFSVVDD17 pKa = 3.37 EE18 pKa = 4.4 RR19 pKa = 11.84 TRR21 pKa = 11.84 VIPEE25 pKa = 3.69 RR26 pKa = 11.84 HH27 pKa = 4.94 VVLPHH32 pKa = 5.85 GEE34 pKa = 4.07 RR35 pKa = 11.84 AILPTPAPGSSVTTAQHH52 pKa = 5.57 RR53 pKa = 11.84 TPSEE57 pKa = 3.29 IRR59 pKa = 11.84 ADD61 pKa = 3.49 HH62 pKa = 6.74 AGLTEE67 pKa = 3.77 AVRR70 pKa = 11.84 RR71 pKa = 11.84 VSLQGEE77 pKa = 4.44 SRR79 pKa = 11.84 RR80 pKa = 11.84 TTDD83 pKa = 2.57 VGLRR87 pKa = 11.84 GAAHH91 pKa = 6.0 SRR93 pKa = 11.84 VSRR96 pKa = 11.84 ASVRR100 pKa = 11.84 PAATEE105 pKa = 3.88 EE106 pKa = 4.28 GASAVSSRR114 pKa = 11.84 RR115 pKa = 11.84 SEE117 pKa = 4.03 NVGRR121 pKa = 11.84 TGEE124 pKa = 4.35 SVTTLPVYY132 pKa = 10.56 RR133 pKa = 11.84 SAEE136 pKa = 3.98 EE137 pKa = 3.97 LASSNIPQRR146 pKa = 11.84 GDD148 pKa = 3.76 ANVLWRR154 pKa = 11.84 DD155 pKa = 3.26 QTVRR159 pKa = 11.84 GDD161 pKa = 3.77 LEE163 pKa = 4.77 SPVHH167 pKa = 6.42 SGATGIPRR175 pKa = 11.84 VASTATNQQEE185 pKa = 4.25 GSYY188 pKa = 10.85 GPEE191 pKa = 3.62 NVLDD195 pKa = 4.04 FQVPPTYY202 pKa = 10.79 VGVPGAAVGNTGGGGSEE219 pKa = 3.73 PTVVVEE225 pKa = 4.54 RR226 pKa = 11.84 PRR228 pKa = 11.84 GRR230 pKa = 11.84 RR231 pKa = 11.84 SVRR234 pKa = 11.84 GTRR237 pKa = 11.84 RR238 pKa = 11.84 ATDD241 pKa = 3.35 SVGVLTSALAALPEE255 pKa = 4.71 GSSFTISVAEE265 pKa = 4.05 EE266 pKa = 3.78 VAPDD270 pKa = 3.69 GTARR274 pKa = 11.84 SQASVSVTASSRR286 pKa = 11.84 ASGSTSSRR294 pKa = 11.84 RR295 pKa = 11.84 VRR297 pKa = 11.84 EE298 pKa = 3.85 VGDD301 pKa = 3.39 GHH303 pKa = 5.6 EE304 pKa = 4.13 HH305 pKa = 5.08 HH306 pKa = 6.9 HH307 pKa = 6.35 RR308 pKa = 11.84 RR309 pKa = 11.84 HH310 pKa = 5.28 KK311 pKa = 10.26 RR312 pKa = 11.84 DD313 pKa = 3.34 RR314 pKa = 11.84 RR315 pKa = 11.84 DD316 pKa = 3.22 SGVTFAEE323 pKa = 4.5 WVAGRR328 pKa = 11.84 RR329 pKa = 11.84 KK330 pKa = 10.04
Molecular weight: 34.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.355
IPC_protein 10.701
Toseland 10.701
ProMoST 11.038
Dawson 10.76
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 10.496
Grimsley 10.818
Solomon 11.125
Lehninger 11.052
Nozaki 10.687
DTASelect 10.672
Thurlkill 10.716
EMBOSS 11.169
Sillero 10.73
Patrickios 10.204
IPC_peptide 11.125
IPC2_peptide 10.043
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
3294
34
771
366.0
39.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.472 ± 0.853
1.791 ± 0.676
6.132 ± 0.473
5.373 ± 0.427
3.491 ± 0.347
8.075 ± 0.939
2.398 ± 0.318
3.279 ± 0.369
3.127 ± 0.598
7.832 ± 0.864
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.295
2.155 ± 0.246
5.556 ± 0.397
2.975 ± 0.288
8.409 ± 0.75
9.624 ± 0.646
5.313 ± 0.606
9.866 ± 0.765
0.88 ± 0.108
2.247 ± 0.439
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here