Candida tropicalis (strain ATCC MYA-3404 / T1) (Yeast)
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6226 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5M4U6|C5M4U6_CANTT Hyphal_reg_CWP domain-containing protein (Fragment) OS=Candida tropicalis (strain ATCC MYA-3404 / T1) OX=294747 GN=CTRG_01924 PE=4 SV=1
MM1 pKa = 7.57 LLLRR5 pKa = 11.84 PISLTLVFLFNYY17 pKa = 9.96 ALSKK21 pKa = 10.77 DD22 pKa = 3.59 ITVDD26 pKa = 3.19 TVDD29 pKa = 3.92 RR30 pKa = 11.84 GSISLSVGDD39 pKa = 4.27 INIHH43 pKa = 6.13 TGASWSIINNAVSALAGSLNVEE65 pKa = 4.21 SDD67 pKa = 2.96 AGFYY71 pKa = 8.7 ITSTSPLIGLDD82 pKa = 3.34 VTLLSTLGSIVNDD95 pKa = 4.98 GIIAFNSVKK104 pKa = 10.6 SLTSSTYY111 pKa = 10.11 NLVGAKK117 pKa = 9.94 FEE119 pKa = 5.2 NNGEE123 pKa = 4.21 MYY125 pKa = 10.64 LGASGVLSGTMSITSLSWKK144 pKa = 10.43 NSGLLVFYY152 pKa = 10.52 QNQRR156 pKa = 11.84 NSGDD160 pKa = 3.47 VRR162 pKa = 11.84 LGTPLGSITNDD173 pKa = 3.28 GQICLINQVYY183 pKa = 9.59 RR184 pKa = 11.84 QTNDD188 pKa = 2.54 IKK190 pKa = 11.25 GSGCITAQKK199 pKa = 10.64 NSTIYY204 pKa = 10.21 IPSAIQSFDD213 pKa = 3.57 TQQSIYY219 pKa = 10.62 LKK221 pKa = 10.24 DD222 pKa = 3.72 SEE224 pKa = 4.82 SSIIVQALSTAQTFNVYY241 pKa = 10.52 GFGNGNKK248 pKa = 9.48 IGLTVPLVGNLIPSYY263 pKa = 9.93 PAYY266 pKa = 9.78 KK267 pKa = 9.83 YY268 pKa = 10.72 DD269 pKa = 3.69 EE270 pKa = 4.38 STGILTLRR278 pKa = 11.84 NLLLKK283 pKa = 10.77 QNFNIGLGYY292 pKa = 10.22 SSSLFQIVTDD302 pKa = 4.06 SGIGLPSTLLGSVQYY317 pKa = 10.41 NGPVPEE323 pKa = 4.33 RR324 pKa = 11.84 SISATCQIEE333 pKa = 4.71 CKK335 pKa = 9.53 EE336 pKa = 4.1 LPEE339 pKa = 4.95 APGVEE344 pKa = 3.97 PTEE347 pKa = 4.07 YY348 pKa = 10.1 LTTLTSTLSDD358 pKa = 3.25 GSVTTEE364 pKa = 3.68 SGIVDD369 pKa = 3.75 VTTDD373 pKa = 4.2 SSGSWYY379 pKa = 6.9 TTTSLFPPLSSSSSEE394 pKa = 4.17 VVSSSSEE401 pKa = 3.75 IVSSTEE407 pKa = 3.98 RR408 pKa = 11.84 EE409 pKa = 3.87 SSLQSFSEE417 pKa = 4.35 YY418 pKa = 10.48 FNSSSSYY425 pKa = 10.51 AFEE428 pKa = 4.2 STSSILEE435 pKa = 4.29 SSSHH439 pKa = 6.4 PSSEE443 pKa = 4.2 VSSTQDD449 pKa = 3.37 DD450 pKa = 3.69 ASEE453 pKa = 5.03 SNFSTSSLISEE464 pKa = 4.55 SNVLEE469 pKa = 4.39 SSDD472 pKa = 3.94 ASATSDD478 pKa = 3.3 VSSASDD484 pKa = 2.95 ISSASDD490 pKa = 3.39 ASDD493 pKa = 3.58 SDD495 pKa = 3.94 PSSTSDD501 pKa = 3.12 VSSASDD507 pKa = 2.98 ISSASDD513 pKa = 2.88 ISSTSEE519 pKa = 3.52 IASSSNTSSASDD531 pKa = 2.96 ISTSSDD537 pKa = 2.83 ASEE540 pKa = 4.63 SNASATSGASSASDD554 pKa = 2.94 ARR556 pKa = 11.84 EE557 pKa = 4.15 SNASATSGASDD568 pKa = 3.69 ASEE571 pKa = 4.21 SDD573 pKa = 3.36 ASATSGASDD582 pKa = 3.69 ASEE585 pKa = 4.21 SDD587 pKa = 3.36 ASATSGASDD596 pKa = 3.23 ASEE599 pKa = 4.44 SNASATSGASDD610 pKa = 3.23 ASEE613 pKa = 4.44 SNASATSGASSASDD627 pKa = 3.09 ASEE630 pKa = 4.01 SDD632 pKa = 3.36 ASATSGASEE641 pKa = 3.94 ASEE644 pKa = 4.53 SNASATSGASSASDD658 pKa = 3.04 ASSASDD664 pKa = 3.44 ANKK667 pKa = 10.61 SNAPATSGASDD678 pKa = 3.52 ASEE681 pKa = 4.24 SDD683 pKa = 3.37 ASSTSDD689 pKa = 3.13 ASPSYY694 pKa = 10.98 NHH696 pKa = 6.91 IEE698 pKa = 4.1 STEE701 pKa = 4.07 SDD703 pKa = 3.58 VQAEE707 pKa = 4.43 FTTTWTTTNSDD718 pKa = 3.13 GSVEE722 pKa = 4.21 TEE724 pKa = 3.89 SGIVSQSGSSFTTLTTFPPEE744 pKa = 3.78 YY745 pKa = 10.12 DD746 pKa = 3.59 VPSS749 pKa = 3.61
Molecular weight: 76.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.643
IPC_protein 3.668
Toseland 3.439
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.49
Grimsley 3.35
Solomon 3.643
Lehninger 3.605
Nozaki 3.757
DTASelect 3.986
Thurlkill 3.49
EMBOSS 3.579
Sillero 3.783
Patrickios 1.087
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.711
Protein with the highest isoelectric point:
>tr|C5M514|C5M514_CANTT Zn(2)-C6 fungal-type domain-containing protein OS=Candida tropicalis (strain ATCC MYA-3404 / T1) OX=294747 GN=CTRG_01992 PE=4 SV=1
MM1 pKa = 7.35 IVEE4 pKa = 5.08 RR5 pKa = 11.84 IKK7 pKa = 10.43 MAIQFQVIVLSALIIIISQNWINVVLMIRR36 pKa = 11.84 IRR38 pKa = 11.84 LSHH41 pKa = 5.57 VVIGHH46 pKa = 5.13 GTEE49 pKa = 3.78 SQYY52 pKa = 11.42 LVPHH56 pKa = 5.89 QLLPVKK62 pKa = 10.32 FHH64 pKa = 5.91 HH65 pKa = 6.31 HH66 pKa = 4.18 QVKK69 pKa = 9.97 KK70 pKa = 9.85 FHH72 pKa = 6.12 HH73 pKa = 6.38 HH74 pKa = 4.83 PVKK77 pKa = 10.38 RR78 pKa = 11.84 FHH80 pKa = 6.09 LHH82 pKa = 3.58 QVKK85 pKa = 9.94 RR86 pKa = 11.84 FHH88 pKa = 6.64 LHH90 pKa = 4.78 HH91 pKa = 6.11 QRR93 pKa = 11.84 KK94 pKa = 7.87 FHH96 pKa = 5.28 QAPVKK101 pKa = 10.12 RR102 pKa = 11.84 SHH104 pKa = 5.4 QAPVKK109 pKa = 10.07 RR110 pKa = 11.84 SHH112 pKa = 5.34 QVPVKK117 pKa = 10.16 RR118 pKa = 11.84 FHH120 pKa = 6.01 LHH122 pKa = 3.96 QVKK125 pKa = 9.94 KK126 pKa = 9.51 FHH128 pKa = 5.9 QAPVKK133 pKa = 10.18 KK134 pKa = 10.11 FHH136 pKa = 6.22 LHH138 pKa = 4.7 PVKK141 pKa = 10.39 RR142 pKa = 11.84 FHH144 pKa = 6.82 LHH146 pKa = 4.81 HH147 pKa = 6.11 QRR149 pKa = 11.84 KK150 pKa = 7.87 FHH152 pKa = 5.28 QAPVKK157 pKa = 10.2 RR158 pKa = 11.84 SHH160 pKa = 5.34 QVPVKK165 pKa = 10.0 RR166 pKa = 11.84 FHH168 pKa = 5.98 HH169 pKa = 6.16 HH170 pKa = 4.8 PVKK173 pKa = 10.44 RR174 pKa = 11.84 FHH176 pKa = 6.67 LHH178 pKa = 4.81 HH179 pKa = 6.11 QRR181 pKa = 11.84 KK182 pKa = 7.87 FHH184 pKa = 5.28 QAPVKK189 pKa = 10.2 RR190 pKa = 11.84 SHH192 pKa = 5.47 QVPVKK197 pKa = 10.13 KK198 pKa = 10.23 FHH200 pKa = 6.04 LRR202 pKa = 11.84 QVKK205 pKa = 9.87 RR206 pKa = 11.84 SHH208 pKa = 5.15 QAPVKK213 pKa = 10.07 RR214 pKa = 11.84 SHH216 pKa = 5.34 QVPVKK221 pKa = 10.16 RR222 pKa = 11.84 FHH224 pKa = 5.92 LHH226 pKa = 3.58 QVKK229 pKa = 10.08 RR230 pKa = 11.84 SHH232 pKa = 5.39 QAPVKK237 pKa = 10.07 RR238 pKa = 11.84 SHH240 pKa = 5.34 QVPVKK245 pKa = 10.16 RR246 pKa = 11.84 FHH248 pKa = 6.01 LHH250 pKa = 3.96 QVKK253 pKa = 9.94 KK254 pKa = 9.51 FHH256 pKa = 5.9 QAPVKK261 pKa = 10.18 KK262 pKa = 10.11 FHH264 pKa = 6.22 LHH266 pKa = 4.7 PVKK269 pKa = 10.32 RR270 pKa = 11.84 FHH272 pKa = 6.16 LHH274 pKa = 3.58 QVKK277 pKa = 9.88 RR278 pKa = 11.84 FHH280 pKa = 6.04 LHH282 pKa = 3.58 QVKK285 pKa = 10.05 RR286 pKa = 11.84 SHH288 pKa = 5.84 LHH290 pKa = 3.74 QVKK293 pKa = 9.99 RR294 pKa = 11.84 SHH296 pKa = 5.84 LHH298 pKa = 3.71 QVKK301 pKa = 9.97 RR302 pKa = 11.84 SHH304 pKa = 5.39 QAPVKK309 pKa = 10.07 RR310 pKa = 11.84 SHH312 pKa = 5.38 QVPVKK317 pKa = 10.26 KK318 pKa = 10.84 VPTKK322 pKa = 10.44 FQQ324 pKa = 3.37
Molecular weight: 39.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.482
IPC2_protein 11.184
IPC_protein 12.632
Toseland 12.822
ProMoST 13.29
Dawson 12.822
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.72
Grimsley 12.866
Solomon 13.305
Lehninger 13.203
Nozaki 12.822
DTASelect 12.808
Thurlkill 12.822
EMBOSS 13.305
Sillero 12.822
Patrickios 12.427
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.049
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6226
0
6226
3018650
50
3808
484.8
54.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.05 ± 0.037
1.101 ± 0.009
5.983 ± 0.026
6.759 ± 0.033
4.491 ± 0.021
5.091 ± 0.033
2.072 ± 0.013
7.064 ± 0.026
7.222 ± 0.038
9.14 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.882 ± 0.011
6.269 ± 0.027
4.553 ± 0.03
4.272 ± 0.025
3.821 ± 0.018
9.06 ± 0.045
5.992 ± 0.039
5.623 ± 0.024
0.984 ± 0.01
3.571 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here