Nitrososphaera gargensis (strain Ga9.2)

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Nitrososphaerales; Nitrososphaeraceae; Nitrososphaera; Candidatus Nitrososphaera gargensis

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3523 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0IMI5|K0IMI5_NITGG DNA double-strand break repair rad50 ATPase OS=Nitrososphaera gargensis (strain Ga9.2) OX=1237085 GN=rad50 PE=4 SV=1
MM1 pKa = 7.06VLAATVSGTGRR12 pKa = 11.84NDD14 pKa = 3.42TLEE17 pKa = 4.29GTQEE21 pKa = 4.23DD22 pKa = 4.32DD23 pKa = 3.55TIYY26 pKa = 10.75GYY28 pKa = 11.26GGDD31 pKa = 4.26DD32 pKa = 3.91EE33 pKa = 5.31PWGNDD38 pKa = 3.34GNDD41 pKa = 2.93ILYY44 pKa = 10.99GNGGNDD50 pKa = 4.08YY51 pKa = 11.07INDD54 pKa = 3.83GTNGNDD60 pKa = 3.49HH61 pKa = 6.66LHH63 pKa = 5.56GHH65 pKa = 6.12SGNDD69 pKa = 2.99EE70 pKa = 4.1LLAGFGFDD78 pKa = 3.35VLYY81 pKa = 10.99GGSGDD86 pKa = 5.03DD87 pKa = 3.58NMHH90 pKa = 6.98KK91 pKa = 10.31IFGDD95 pKa = 3.35SQMYY99 pKa = 10.43GGTGNDD105 pKa = 3.12IIMGGRR111 pKa = 11.84GGVDD115 pKa = 3.65TISGGNGNDD124 pKa = 3.61KK125 pKa = 10.9LYY127 pKa = 11.28GMLGNDD133 pKa = 4.22VIGGGYY139 pKa = 9.68GDD141 pKa = 4.24NGIDD145 pKa = 4.03GGDD148 pKa = 3.57GNDD151 pKa = 3.53ILRR154 pKa = 11.84GHH156 pKa = 6.83YY157 pKa = 10.8DD158 pKa = 3.27NDD160 pKa = 3.25ILTGGGGADD169 pKa = 3.2KK170 pKa = 9.7FYY172 pKa = 10.65CGSRR176 pKa = 11.84IDD178 pKa = 3.68TVTDD182 pKa = 4.11FNAAEE187 pKa = 4.8GDD189 pKa = 3.98TKK191 pKa = 11.13LGDD194 pKa = 3.91CEE196 pKa = 4.77NYY198 pKa = 10.74

Molecular weight: 20.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0IMY5|K0IMY5_NITGG Uncharacterized protein OS=Nitrososphaera gargensis (strain Ga9.2) OX=1237085 GN=Ngar_c14990 PE=4 SV=1
MM1 pKa = 7.31LLVRR5 pKa = 11.84ILHH8 pKa = 6.37LLINLQIILLQAVLKK23 pKa = 10.46NNQQITVHH31 pKa = 6.4LLVIQRR37 pKa = 11.84TITIPRR43 pKa = 11.84LRR45 pKa = 11.84MVKK48 pKa = 9.84NRR50 pKa = 11.84NHH52 pKa = 6.82RR53 pKa = 11.84MPLHH57 pKa = 5.95HH58 pKa = 7.02LKK60 pKa = 10.74VSTIILLTRR69 pKa = 11.84QPRR72 pKa = 11.84MNNHH76 pKa = 5.78QKK78 pKa = 10.42LQVISHH84 pKa = 6.16HH85 pKa = 7.68LEE87 pKa = 3.56MLMLHH92 pKa = 5.71QAMLLLQPHH101 pKa = 7.62LLTVQISKK109 pKa = 10.43VSSNHH114 pKa = 4.46QLRR117 pKa = 11.84MM118 pKa = 3.53

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3523

0

3523

756973

25

2466

214.9

23.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.498 ± 0.05

1.029 ± 0.018

5.443 ± 0.036

6.35 ± 0.049

3.875 ± 0.033

6.937 ± 0.042

1.935 ± 0.022

7.261 ± 0.039

6.661 ± 0.053

8.717 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.904 ± 0.025

3.972 ± 0.031

3.953 ± 0.029

3.319 ± 0.028

5.4 ± 0.037

6.859 ± 0.051

5.307 ± 0.047

7.301 ± 0.036

0.988 ± 0.016

3.288 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski