Leuconostoc phage P793
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4I6A1|M4I6A1_9CAUD Putative phage tail tape measure protein OS=Leuconostoc phage P793 OX=1262522 GN=P793_018 PE=4 SV=1
MM1 pKa = 7.0 TTFDD5 pKa = 3.51 YY6 pKa = 10.99 QKK8 pKa = 11.03 YY9 pKa = 10.85 LNVDD13 pKa = 3.54 DD14 pKa = 4.65 NTYY17 pKa = 10.84 SDD19 pKa = 3.03 WSGTIEE25 pKa = 4.08 EE26 pKa = 5.23 LEE28 pKa = 3.87 NSAINRR34 pKa = 11.84 AQRR37 pKa = 11.84 LNPTLTIDD45 pKa = 3.63 TTNKK49 pKa = 10.17 NLYY52 pKa = 9.14 EE53 pKa = 4.06 YY54 pKa = 9.58 AKK56 pKa = 9.37 EE57 pKa = 3.94 VIYY60 pKa = 10.51 IGIFGDD66 pKa = 3.92 TLTPALWQYY75 pKa = 11.21 HH76 pKa = 5.65 DD77 pKa = 4.64 SKK79 pKa = 11.05 MSDD82 pKa = 2.97 LEE84 pKa = 4.45 EE85 pKa = 4.19 YY86 pKa = 9.86 FRR88 pKa = 11.84 WWQEE92 pKa = 3.34
Molecular weight: 11.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.939
IPC2_protein 4.253
IPC_protein 4.139
Toseland 3.961
ProMoST 4.228
Dawson 4.113
Bjellqvist 4.329
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.05
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.024
Sillero 4.253
Patrickios 1.964
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.179
Protein with the highest isoelectric point:
>tr|M4I6M9|M4I6M9_9CAUD Receptor-binding tail protein OS=Leuconostoc phage P793 OX=1262522 GN=P793_021 PE=4 SV=1
MM1 pKa = 7.24 VKK3 pKa = 9.98 RR4 pKa = 11.84 VNSIVDD10 pKa = 3.2 IANGTKK16 pKa = 9.92 KK17 pKa = 10.2 FAIAKK22 pKa = 9.36 VLEE25 pKa = 4.47 KK26 pKa = 10.02 RR27 pKa = 11.84 DD28 pKa = 3.47 KK29 pKa = 10.01 TAQQASSQSNSALGVWHH46 pKa = 6.49 VMAPKK51 pKa = 10.48 GRR53 pKa = 11.84 TGQMVSGVSVKK64 pKa = 10.1 HH65 pKa = 5.14 GKK67 pKa = 9.88 YY68 pKa = 10.06 YY69 pKa = 10.37 SVVYY73 pKa = 7.64 PTHH76 pKa = 7.86 DD77 pKa = 3.42 YY78 pKa = 10.77 RR79 pKa = 11.84 KK80 pKa = 9.34 EE81 pKa = 3.61 GTAFMEE87 pKa = 4.46 IVNVQNKK94 pKa = 9.62 RR95 pKa = 11.84 GVHH98 pKa = 5.45 AGFFNRR104 pKa = 11.84 YY105 pKa = 5.74 RR106 pKa = 11.84 TQLGKK111 pKa = 10.74 SFLTSVKK118 pKa = 10.6
Molecular weight: 13.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.242
IPC2_protein 9.897
IPC_protein 10.16
Toseland 10.672
ProMoST 10.262
Dawson 10.774
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.345
Grimsley 10.818
Solomon 10.818
Lehninger 10.789
Nozaki 10.628
DTASelect 10.394
Thurlkill 10.657
EMBOSS 11.038
Sillero 10.687
Patrickios 11.082
IPC_peptide 10.818
IPC2_peptide 8.946
IPC2.peptide.svr19 8.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
38
0
38
8209
48
818
216.0
24.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.456 ± 0.466
0.171 ± 0.077
6.529 ± 0.428
4.714 ± 0.465
4.641 ± 0.225
6.822 ± 0.66
1.474 ± 0.237
7.309 ± 0.261
7.09 ± 0.431
8.089 ± 0.38
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.148
6.785 ± 0.342
3.131 ± 0.25
4.385 ± 0.247
3.07 ± 0.223
7.565 ± 0.476
7.516 ± 0.375
6.456 ± 0.336
1.06 ± 0.164
4.519 ± 0.473
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here