Ruminococcus sp. CAG:108

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5DQN4|R5DQN4_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:108 OX=1262950 GN=BN462_00005 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.19KK3 pKa = 9.69IKK5 pKa = 10.33KK6 pKa = 9.28LALLLAICMVGTVGLVGCGNDD27 pKa = 3.75GDD29 pKa = 4.42SSSKK33 pKa = 10.78NEE35 pKa = 3.89SSATQTADD43 pKa = 4.04DD44 pKa = 4.56NANSADD50 pKa = 4.27DD51 pKa = 3.77SAADD55 pKa = 3.99SKK57 pKa = 11.68DD58 pKa = 3.25GDD60 pKa = 3.77SSASVVGTWTVTALEE75 pKa = 4.62GTDD78 pKa = 3.54GTAVSIDD85 pKa = 4.26DD86 pKa = 3.77YY87 pKa = 11.25ATANGVDD94 pKa = 4.34PEE96 pKa = 4.52TLKK99 pKa = 10.2CTYY102 pKa = 9.92TFADD106 pKa = 4.27DD107 pKa = 3.94GTFSGEE113 pKa = 3.89MSGVTVSGNYY123 pKa = 8.23TFEE126 pKa = 4.2GTDD129 pKa = 3.17LVMTVGEE136 pKa = 4.06QQFNYY141 pKa = 10.12TYY143 pKa = 11.11DD144 pKa = 3.71ADD146 pKa = 4.35NDD148 pKa = 3.87MLKK151 pKa = 9.19YY152 pKa = 9.31TDD154 pKa = 4.56PNTGLSSYY162 pKa = 10.02CEE164 pKa = 3.99RR165 pKa = 11.84QQQ167 pKa = 3.84

Molecular weight: 17.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5E4U9|R5E4U9_9FIRM Glycosyltransferase OS=Ruminococcus sp. CAG:108 OX=1262950 GN=BN462_01231 PE=4 SV=1
MM1 pKa = 8.1ADD3 pKa = 3.28RR4 pKa = 11.84PMNRR8 pKa = 11.84GRR10 pKa = 11.84KK11 pKa = 8.6ARR13 pKa = 11.84KK14 pKa = 8.66KK15 pKa = 9.55VCGFCVDD22 pKa = 3.21KK23 pKa = 11.52VEE25 pKa = 4.75NIDD28 pKa = 3.57YY29 pKa = 10.94KK30 pKa = 11.15DD31 pKa = 3.08IARR34 pKa = 11.84LRR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 8.42MSEE41 pKa = 3.7RR42 pKa = 11.84GKK44 pKa = 10.14ILPRR48 pKa = 11.84RR49 pKa = 11.84VTGTCARR56 pKa = 11.84HH57 pKa = 4.41QRR59 pKa = 11.84EE60 pKa = 4.06LTVAIKK66 pKa = 10.33RR67 pKa = 11.84ARR69 pKa = 11.84HH70 pKa = 5.37LALLPYY76 pKa = 9.54TADD79 pKa = 3.2

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1917

0

1917

592298

29

4076

309.0

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.176 ± 0.052

1.748 ± 0.027

6.186 ± 0.045

6.62 ± 0.061

4.47 ± 0.045

6.687 ± 0.056

1.463 ± 0.023

7.662 ± 0.052

7.733 ± 0.051

8.34 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.027

5.41 ± 0.048

3.134 ± 0.034

2.531 ± 0.027

3.697 ± 0.049

6.696 ± 0.061

5.809 ± 0.06

7.105 ± 0.044

0.705 ± 0.019

4.14 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski