Amino acid dipepetide frequency for Ruminococcus sp. CAG:108

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.609AlaAla: 5.609 ± 0.125	1.153AlaCys: 1.153 ± 0.049	4.918AlaAsp: 4.918 ± 0.102	5.232AlaGlu: 5.232 ± 0.098	3.348AlaPhe: 3.348 ± 0.087	4.653AlaGly: 4.653 ± 0.105	0.944AlaHis: 0.944 ± 0.039	5.001AlaIle: 5.001 ± 0.11	5.543AlaLys: 5.543 ± 0.111	6.38AlaLeu: 6.38 ± 0.103	1.979AlaMet: 1.979 ± 0.069	2.955AlaAsn: 2.955 ± 0.071	2.077AlaPro: 2.077 ± 0.069	2.088AlaGln: 2.088 ± 0.068	2.403AlaArg: 2.403 ± 0.068	3.89AlaSer: 3.89 ± 0.089	3.745AlaThr: 3.745 ± 0.1	6.866AlaVal: 6.866 ± 0.103	0.397AlaTrp: 0.397 ± 0.028	2.581AlaTyr: 2.581 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	1.29CysAla: 1.29 ± 0.049	0.371CysCys: 0.371 ± 0.031	1.373CysAsp: 1.373 ± 0.049	1.18CysGlu: 1.18 ± 0.046	0.711CysPhe: 0.711 ± 0.034	1.737CysGly: 1.737 ± 0.058	0.279CysHis: 0.279 ± 0.019	1.182CysIle: 1.182 ± 0.048	1.271CysLys: 1.271 ± 0.048	1.165CysLeu: 1.165 ± 0.046	0.407CysMet: 0.407 ± 0.026	0.805CysAsn: 0.805 ± 0.041	0.625CysPro: 0.625 ± 0.037	0.328CysGln: 0.328 ± 0.023	0.665CysArg: 0.665 ± 0.035	1.155CysSer: 1.155 ± 0.044	0.957CysThr: 0.957 ± 0.04	1.298CysVal: 1.298 ± 0.045	0.103CysTrp: 0.103 ± 0.014	0.579CysTyr: 0.579 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.069AspAla: 4.069 ± 0.092	1.015AspCys: 1.015 ± 0.049	4.028AspAsp: 4.028 ± 0.104	5.155AspGlu: 5.155 ± 0.106	3.248AspPhe: 3.248 ± 0.075	4.876AspGly: 4.876 ± 0.12	0.615AspHis: 0.615 ± 0.038	4.95AspIle: 4.95 ± 0.102	4.839AspLys: 4.839 ± 0.102	4.266AspLeu: 4.266 ± 0.1	1.82AspMet: 1.82 ± 0.048	3.446AspAsn: 3.446 ± 0.091	1.391AspPro: 1.391 ± 0.051	0.743AspGln: 0.743 ± 0.037	2.333AspArg: 2.333 ± 0.067	3.959AspSer: 3.959 ± 0.082	3.524AspThr: 3.524 ± 0.085	4.087AspVal: 4.087 ± 0.093	0.414AspTrp: 0.414 ± 0.029	3.08AspTyr: 3.08 ± 0.075	0.0AspXaa: 0.0 ± 0.0
Glu	3.684GluAla: 3.684 ± 0.08	0.994GluCys: 0.994 ± 0.042	3.353GluAsp: 3.353 ± 0.078	4.749GluGlu: 4.749 ± 0.108	2.713GluPhe: 2.713 ± 0.081	3.372GluGly: 3.372 ± 0.081	1.102GluHis: 1.102 ± 0.046	5.764GluIle: 5.764 ± 0.127	6.441GluLys: 6.441 ± 0.132	5.96GluLeu: 5.96 ± 0.119	2.016GluMet: 2.016 ± 0.056	5.134GluAsn: 5.134 ± 0.093	1.766GluPro: 1.766 ± 0.065	2.239GluGln: 2.239 ± 0.068	2.603GluArg: 2.603 ± 0.072	3.718GluSer: 3.718 ± 0.086	3.367GluThr: 3.367 ± 0.085	3.77GluVal: 3.77 ± 0.1	0.51GluTrp: 0.51 ± 0.034	2.948GluTyr: 2.948 ± 0.071	0.002GluXaa: 0.002 ± 0.002
Phe	3.404PheAla: 3.404 ± 0.079	1.011PheCys: 1.011 ± 0.04	3.304PheAsp: 3.304 ± 0.087	2.938PheGlu: 2.938 ± 0.073	2.007PhePhe: 2.007 ± 0.068	3.204PheGly: 3.204 ± 0.089	0.542PheHis: 0.542 ± 0.026	3.238PheIle: 3.238 ± 0.078	2.782PheLys: 2.782 ± 0.078	3.478PheLeu: 3.478 ± 0.106	1.197PheMet: 1.197 ± 0.053	2.17PheAsn: 2.17 ± 0.067	1.366PhePro: 1.366 ± 0.051	0.869PheGln: 0.869 ± 0.04	1.43PheArg: 1.43 ± 0.054	3.605PheSer: 3.605 ± 0.084	2.681PheThr: 2.681 ± 0.076	3.355PheVal: 3.355 ± 0.082	0.336PheTrp: 0.336 ± 0.025	1.786PheTyr: 1.786 ± 0.063	0.0PheXaa: 0.0 ± 0.0
Gly	4.759GlyAla: 4.759 ± 0.116	1.26GlyCys: 1.26 ± 0.043	3.934GlyAsp: 3.934 ± 0.089	4.329GlyGlu: 4.329 ± 0.08	3.095GlyPhe: 3.095 ± 0.075	4.648GlyGly: 4.648 ± 0.112	0.999GlyHis: 0.999 ± 0.039	5.691GlyIle: 5.691 ± 0.098	5.585GlyLys: 5.585 ± 0.105	5.079GlyLeu: 5.079 ± 0.097	2.036GlyMet: 2.036 ± 0.064	3.37GlyAsn: 3.37 ± 0.086	0.967GlyPro: 0.967 ± 0.044	1.577GlyGln: 1.577 ± 0.053	2.551GlyArg: 2.551 ± 0.073	4.15GlySer: 4.15 ± 0.096	4.148GlyThr: 4.148 ± 0.103	5.131GlyVal: 5.131 ± 0.104	0.577GlyTrp: 0.577 ± 0.037	2.98GlyTyr: 2.98 ± 0.067	0.0GlyXaa: 0.0 ± 0.0
His	0.915HisAla: 0.915 ± 0.041	0.326HisCys: 0.326 ± 0.022	0.794HisAsp: 0.794 ± 0.037	0.777HisGlu: 0.777 ± 0.038	0.694HisPhe: 0.694 ± 0.036	1.238HisGly: 1.238 ± 0.044	0.324HisHis: 0.324 ± 0.032	1.251HisIle: 1.251 ± 0.044	1.003HisLys: 1.003 ± 0.041	1.153HisLeu: 1.153 ± 0.041	0.377HisMet: 0.377 ± 0.027	0.89HisAsn: 0.89 ± 0.039	0.692HisPro: 0.692 ± 0.036	0.309HisGln: 0.309 ± 0.025	0.65HisArg: 0.65 ± 0.035	0.915HisSer: 0.915 ± 0.039	0.94HisThr: 0.94 ± 0.036	0.613HisVal: 0.613 ± 0.031	0.125HisTrp: 0.125 ± 0.014	0.647HisTyr: 0.647 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.847IleAla: 5.847 ± 0.11	1.565IleCys: 1.565 ± 0.051	4.965IleAsp: 4.965 ± 0.101	4.819IleGlu: 4.819 ± 0.107	3.338IlePhe: 3.338 ± 0.102	4.896IleGly: 4.896 ± 0.086	1.03IleHis: 1.03 ± 0.042	6.178IleIle: 6.178 ± 0.128	5.68IleLys: 5.68 ± 0.1	6.259IleLeu: 6.259 ± 0.131	1.926IleMet: 1.926 ± 0.055	3.812IleAsn: 3.812 ± 0.088	2.98IlePro: 2.98 ± 0.08	1.594IleGln: 1.594 ± 0.051	2.787IleArg: 2.787 ± 0.074	5.718IleSer: 5.718 ± 0.121	4.712IleThr: 4.712 ± 0.087	5.394IleVal: 5.394 ± 0.085	0.441IleTrp: 0.441 ± 0.032	2.683IleTyr: 2.683 ± 0.081	0.0IleXaa: 0.0 ± 0.0
Lys	5.47LysAla: 5.47 ± 0.102	1.016LysCys: 1.016 ± 0.045	4.125LysAsp: 4.125 ± 0.095	5.274LysGlu: 5.274 ± 0.103	2.779LysPhe: 2.779 ± 0.081	4.361LysGly: 4.361 ± 0.082	1.141LysHis: 1.141 ± 0.043	6.286LysIle: 6.286 ± 0.119	7.007LysLys: 7.007 ± 0.122	6.331LysLeu: 6.331 ± 0.104	2.178LysMet: 2.178 ± 0.065	5.04LysAsn: 5.04 ± 0.093	2.355LysPro: 2.355 ± 0.07	2.288LysGln: 2.288 ± 0.072	3.005LysArg: 3.005 ± 0.082	5.197LysSer: 5.197 ± 0.096	4.862LysThr: 4.862 ± 0.096	4.548LysVal: 4.548 ± 0.084	0.54LysTrp: 0.54 ± 0.029	3.53LysTyr: 3.53 ± 0.079	0.0LysXaa: 0.0 ± 0.0
Leu	6.083LeuAla: 6.083 ± 0.113	1.818LeuCys: 1.818 ± 0.064	5.301LeuAsp: 5.301 ± 0.1	5.234LeuGlu: 5.234 ± 0.105	3.827LeuPhe: 3.827 ± 0.101	5.242LeuGly: 5.242 ± 0.098	1.241LeuHis: 1.241 ± 0.043	6.093LeuIle: 6.093 ± 0.122	6.431LeuLys: 6.431 ± 0.107	7.268LeuLeu: 7.268 ± 0.142	2.281LeuMet: 2.281 ± 0.062	4.277LeuAsn: 4.277 ± 0.09	3.076LeuPro: 3.076 ± 0.08	1.872LeuGln: 1.872 ± 0.064	3.091LeuArg: 3.091 ± 0.081	6.401LeuSer: 6.401 ± 0.118	4.822LeuThr: 4.822 ± 0.094	5.355LeuVal: 5.355 ± 0.094	0.623LeuTrp: 0.623 ± 0.034	3.059LeuTyr: 3.059 ± 0.077	0.0LeuXaa: 0.0 ± 0.0
Met	1.969MetAla: 1.969 ± 0.06	0.432MetCys: 0.432 ± 0.028	1.29MetAsp: 1.29 ± 0.044	1.371MetGlu: 1.371 ± 0.053	1.256MetPhe: 1.256 ± 0.052	1.704MetGly: 1.704 ± 0.059	0.402MetHis: 0.402 ± 0.03	1.964MetIle: 1.964 ± 0.062	2.318MetLys: 2.318 ± 0.062	2.641MetLeu: 2.641 ± 0.07	0.753MetMet: 0.753 ± 0.043	1.58MetAsn: 1.58 ± 0.056	1.114MetPro: 1.114 ± 0.052	0.893MetGln: 0.893 ± 0.045	1.06MetArg: 1.06 ± 0.041	1.872MetSer: 1.872 ± 0.054	1.69MetThr: 1.69 ± 0.06	1.457MetVal: 1.457 ± 0.053	0.198MetTrp: 0.198 ± 0.019	0.881MetTyr: 0.881 ± 0.038	0.0MetXaa: 0.0 ± 0.0
Asn	4.047AsnAla: 4.047 ± 0.089	0.974AsnCys: 0.974 ± 0.048	2.99AsnAsp: 2.99 ± 0.076	3.346AsnGlu: 3.346 ± 0.079	2.161AsnPhe: 2.161 ± 0.07	4.54AsnGly: 4.54 ± 0.091	0.768AsnHis: 0.768 ± 0.038	4.349AsnIle: 4.349 ± 0.095	3.667AsnLys: 3.667 ± 0.083	4.233AsnLeu: 4.233 ± 0.09	1.438AsnMet: 1.438 ± 0.047	3.134AsnAsn: 3.134 ± 0.092	2.278AsnPro: 2.278 ± 0.062	1.214AsnGln: 1.214 ± 0.046	2.099AsnArg: 2.099 ± 0.069	3.701AsnSer: 3.701 ± 0.08	2.951AsnThr: 2.951 ± 0.075	3.544AsnVal: 3.544 ± 0.085	0.385AsnTrp: 0.385 ± 0.028	2.281AsnTyr: 2.281 ± 0.08	0.0AsnXaa: 0.0 ± 0.0
Pro	1.896ProAla: 1.896 ± 0.058	0.513ProCys: 0.513 ± 0.027	2.342ProAsp: 2.342 ± 0.065	2.69ProGlu: 2.69 ± 0.076	1.538ProPhe: 1.538 ± 0.055	1.42ProGly: 1.42 ± 0.054	0.586ProHis: 0.586 ± 0.032	2.107ProIle: 2.107 ± 0.059	2.237ProLys: 2.237 ± 0.068	2.571ProLeu: 2.571 ± 0.067	0.775ProMet: 0.775 ± 0.033	1.641ProAsn: 1.641 ± 0.057	0.694ProPro: 0.694 ± 0.032	0.979ProGln: 0.979 ± 0.044	0.849ProArg: 0.849 ± 0.041	2.153ProSer: 2.153 ± 0.07	1.852ProThr: 1.852 ± 0.067	2.828ProVal: 2.828 ± 0.072	0.246ProTrp: 0.246 ± 0.023	1.427ProTyr: 1.427 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	1.879GlnAla: 1.879 ± 0.066	0.333GlnCys: 0.333 ± 0.024	0.999GlnAsp: 0.999 ± 0.037	1.275GlnGlu: 1.275 ± 0.052	1.008GlnPhe: 1.008 ± 0.046	1.474GlnGly: 1.474 ± 0.044	0.414GlnHis: 0.414 ± 0.027	2.1GlnIle: 2.1 ± 0.048	2.271GlnLys: 2.271 ± 0.069	2.418GlnLeu: 2.418 ± 0.069	0.712GlnMet: 0.712 ± 0.036	1.668GlnAsn: 1.668 ± 0.061	0.878GlnPro: 0.878 ± 0.051	0.913GlnGln: 0.913 ± 0.052	1.172GlnArg: 1.172 ± 0.047	1.604GlnSer: 1.604 ± 0.059	1.628GlnThr: 1.628 ± 0.052	1.275GlnVal: 1.275 ± 0.048	0.184GlnTrp: 0.184 ± 0.017	1.109GlnTyr: 1.109 ± 0.049	0.002GlnXaa: 0.002 ± 0.002
Arg	2.301ArgAla: 2.301 ± 0.059	0.623ArgCys: 0.623 ± 0.035	2.043ArgAsp: 2.043 ± 0.067	2.666ArgGlu: 2.666 ± 0.076	1.759ArgPhe: 1.759 ± 0.046	2.099ArgGly: 2.099 ± 0.064	0.679ArgHis: 0.679 ± 0.035	3.029ArgIle: 3.029 ± 0.082	3.103ArgLys: 3.103 ± 0.082	3.368ArgLeu: 3.368 ± 0.09	1.021ArgMet: 1.021 ± 0.044	1.984ArgAsn: 1.984 ± 0.065	1.133ArgPro: 1.133 ± 0.044	1.163ArgGln: 1.163 ± 0.049	1.742ArgArg: 1.742 ± 0.078	1.958ArgSer: 1.958 ± 0.048	1.977ArgThr: 1.977 ± 0.057	2.431ArgVal: 2.431 ± 0.062	0.267ArgTrp: 0.267 ± 0.025	1.621ArgTyr: 1.621 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	5.104SerAla: 5.104 ± 0.105	0.961SerCys: 0.961 ± 0.041	4.447SerAsp: 4.447 ± 0.096	4.342SerGlu: 4.342 ± 0.093	3.125SerPhe: 3.125 ± 0.084	5.291SerGly: 5.291 ± 0.109	0.979SerHis: 0.979 ± 0.039	4.423SerIle: 4.423 ± 0.104	4.866SerLys: 4.866 ± 0.104	5.545SerLeu: 5.545 ± 0.109	1.607SerMet: 1.607 ± 0.053	3.161SerAsn: 3.161 ± 0.086	1.901SerPro: 1.901 ± 0.053	1.893SerGln: 1.893 ± 0.058	2.435SerArg: 2.435 ± 0.066	4.667SerSer: 4.667 ± 0.147	3.326SerThr: 3.326 ± 0.1	5.649SerVal: 5.649 ± 0.11	0.473SerTrp: 0.473 ± 0.033	2.767SerTyr: 2.767 ± 0.09	0.0SerXaa: 0.0 ± 0.0
Thr	5.114ThrAla: 5.114 ± 0.112	0.658ThrCys: 0.658 ± 0.035	4.001ThrAsp: 4.001 ± 0.096	3.726ThrGlu: 3.726 ± 0.099	2.387ThrPhe: 2.387 ± 0.061	4.462ThrGly: 4.462 ± 0.104	0.905ThrHis: 0.905 ± 0.034	3.951ThrIle: 3.951 ± 0.096	3.681ThrLys: 3.681 ± 0.086	5.026ThrLeu: 5.026 ± 0.101	1.271ThrMet: 1.271 ± 0.05	2.551ThrAsn: 2.551 ± 0.067	2.188ThrPro: 2.188 ± 0.062	1.499ThrGln: 1.499 ± 0.056	1.683ThrArg: 1.683 ± 0.052	3.513ThrSer: 3.513 ± 0.09	3.519ThrThr: 3.519 ± 0.099	5.303ThrVal: 5.303 ± 0.121	0.339ThrTrp: 0.339 ± 0.026	2.31ThrTyr: 2.31 ± 0.081	0.002ThrXaa: 0.002 ± 0.002
Val	5.104ValAla: 5.104 ± 0.104	1.531ValCys: 1.531 ± 0.054	4.234ValAsp: 4.234 ± 0.111	4.26ValGlu: 4.26 ± 0.09	3.373ValPhe: 3.373 ± 0.092	4.165ValGly: 4.165 ± 0.097	0.932ValHis: 0.932 ± 0.04	5.438ValIle: 5.438 ± 0.102	5.251ValLys: 5.251 ± 0.092	6.299ValLeu: 6.299 ± 0.114	1.935ValMet: 1.935 ± 0.059	3.542ValAsn: 3.542 ± 0.077	2.484ValPro: 2.484 ± 0.071	1.675ValGln: 1.675 ± 0.063	2.543ValArg: 2.543 ± 0.059	5.3ValSer: 5.3 ± 0.119	4.584ValThr: 4.584 ± 0.119	5.05ValVal: 5.05 ± 0.114	0.552ValTrp: 0.552 ± 0.03	2.794ValTyr: 2.794 ± 0.077	0.0ValXaa: 0.0 ± 0.0
Trp	0.415TrpAla: 0.415 ± 0.03	0.127TrpCys: 0.127 ± 0.014	0.431TrpAsp: 0.431 ± 0.028	0.392TrpGlu: 0.392 ± 0.029	0.378TrpPhe: 0.378 ± 0.026	0.5TrpGly: 0.5 ± 0.032	0.147TrpHis: 0.147 ± 0.015	0.466TrpIle: 0.466 ± 0.028	0.476TrpLys: 0.476 ± 0.034	0.685TrpLeu: 0.685 ± 0.035	0.182TrpMet: 0.182 ± 0.018	0.474TrpAsn: 0.474 ± 0.031	0.135TrpPro: 0.135 ± 0.016	0.295TrpGln: 0.295 ± 0.023	0.265TrpArg: 0.265 ± 0.021	0.485TrpSer: 0.485 ± 0.032	0.322TrpThr: 0.322 ± 0.027	0.409TrpVal: 0.409 ± 0.029	0.068TrpTrp: 0.068 ± 0.011	0.395TrpTyr: 0.395 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.803TyrAla: 2.803 ± 0.082	0.799TyrCys: 0.799 ± 0.04	2.985TyrAsp: 2.985 ± 0.07	2.451TyrGlu: 2.451 ± 0.069	1.957TyrPhe: 1.957 ± 0.06	2.983TyrGly: 2.983 ± 0.071	0.603TyrHis: 0.603 ± 0.032	3.162TyrIle: 3.162 ± 0.078	2.884TyrLys: 2.884 ± 0.07	3.27TyrLeu: 3.27 ± 0.079	0.93TyrMet: 0.93 ± 0.04	2.468TyrAsn: 2.468 ± 0.071	1.244TyrPro: 1.244 ± 0.048	0.883TyrGln: 0.883 ± 0.043	1.599TyrArg: 1.599 ± 0.053	3.002TyrSer: 3.002 ± 0.084	2.484TyrThr: 2.484 ± 0.081	2.681TyrVal: 2.681 ± 0.081	0.27TyrTrp: 0.27 ± 0.022	1.947TyrTyr: 1.947 ± 0.069	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.012XaaXaa: 0.012 ± 0.008

Statistics based on 1917 proteins (592298 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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