Inhangapi virus
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R128|A0A0D3R128_9RHAB Nucleocapsid protein OS=Inhangapi virus OX=1620892 PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.65 KK3 pKa = 10.43 FNSSNFCFPDD13 pKa = 3.26 MSSTFKK19 pKa = 10.77 DD20 pKa = 2.87 IRR22 pKa = 11.84 EE23 pKa = 3.95 ISDD26 pKa = 4.69 LIEE29 pKa = 5.83 DD30 pKa = 5.08 DD31 pKa = 5.28 LPPPPPDD38 pKa = 4.23 PPTFDD43 pKa = 3.06 SAEE46 pKa = 4.15 FPNQQLDD53 pKa = 3.2 WGDD56 pKa = 3.52 KK57 pKa = 7.57 VTEE60 pKa = 3.91 EE61 pKa = 4.15 AARR64 pKa = 11.84 GDD66 pKa = 3.7 WEE68 pKa = 4.54 LPPPAGDD75 pKa = 3.16 NVFTFRR81 pKa = 11.84 TSSLKK86 pKa = 10.64 DD87 pKa = 3.25 LEE89 pKa = 4.26 RR90 pKa = 11.84 AAIEE94 pKa = 4.21 SEE96 pKa = 4.62 LINLIAWIYY105 pKa = 10.19 KK106 pKa = 8.19 HH107 pKa = 5.58 THH109 pKa = 5.83 LLLDD113 pKa = 3.82 YY114 pKa = 10.15 RR115 pKa = 11.84 VSDD118 pKa = 4.33 DD119 pKa = 4.91 GIDD122 pKa = 3.31 VLTIEE127 pKa = 4.9 KK128 pKa = 10.65 GEE130 pKa = 4.08 SLEE133 pKa = 4.15 WEE135 pKa = 4.28 NPKK138 pKa = 10.29 PEE140 pKa = 4.16 QPSPCSVNGKK150 pKa = 7.76 TLDD153 pKa = 4.37 DD154 pKa = 4.05 ALADD158 pKa = 3.63 YY159 pKa = 10.73 RR160 pKa = 11.84 EE161 pKa = 4.19 LMKK164 pKa = 10.25 IEE166 pKa = 4.68 PPAKK170 pKa = 9.33 PVPEE174 pKa = 4.08 TSRR177 pKa = 11.84 EE178 pKa = 4.08 SKK180 pKa = 9.37 TVNKK184 pKa = 9.85 NVSYY188 pKa = 10.54 QKK190 pKa = 10.92 EE191 pKa = 4.29 KK192 pKa = 10.32 MGEE195 pKa = 4.09 TEE197 pKa = 4.15 GGKK200 pKa = 10.07 KK201 pKa = 9.83 PGSLYY206 pKa = 10.64 IMFKK210 pKa = 10.38 NGIRR214 pKa = 11.84 FEE216 pKa = 4.09 KK217 pKa = 9.76 RR218 pKa = 11.84 TGHH221 pKa = 5.25 GTLKK225 pKa = 10.46 ISLDD229 pKa = 3.68 TPGITEE235 pKa = 3.83 KK236 pKa = 10.96 AIAEE240 pKa = 4.24 GEE242 pKa = 4.09 NMNLKK247 pKa = 10.68 DD248 pKa = 4.07 GLKK251 pKa = 10.33 KK252 pKa = 10.36 ALKK255 pKa = 10.26 KK256 pKa = 10.61 SGIYY260 pKa = 9.49 KK261 pKa = 10.61 ALALKK266 pKa = 10.59 ADD268 pKa = 3.94 INNPIWPKK276 pKa = 10.1 MDD278 pKa = 3.74 FF279 pKa = 3.61
Molecular weight: 31.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.961
IPC2_protein 4.914
IPC_protein 4.838
Toseland 4.698
ProMoST 4.94
Dawson 4.774
Bjellqvist 4.927
Wikipedia 4.647
Rodwell 4.685
Grimsley 4.609
Solomon 4.774
Lehninger 4.736
Nozaki 4.889
DTASelect 5.041
Thurlkill 4.685
EMBOSS 4.66
Sillero 4.952
Patrickios 4.38
IPC_peptide 4.787
IPC2_peptide 4.94
IPC2.peptide.svr19 4.971
Protein with the highest isoelectric point:
>tr|A0A0D3R1G5|A0A0D3R1G5_9RHAB G OS=Inhangapi virus OX=1620892 PE=4 SV=1
MM1 pKa = 7.6 NITSRR6 pKa = 11.84 INWQSVDD13 pKa = 3.23 PSKK16 pKa = 10.45 WFEE19 pKa = 4.24 GIKK22 pKa = 10.71 DD23 pKa = 3.61 GANSFFSSLKK33 pKa = 10.65 VVFQDD38 pKa = 2.91 TKK40 pKa = 9.71 YY41 pKa = 10.21 WINLFFWLIVAILIMVIVLKK61 pKa = 10.31 FSNYY65 pKa = 10.31 LIGLINQCGACVKK78 pKa = 10.27 MIGNCQCKK86 pKa = 9.83 CKK88 pKa = 10.53 KK89 pKa = 9.47 KK90 pKa = 10.05 AKK92 pKa = 9.9 KK93 pKa = 9.19 RR94 pKa = 11.84 GKK96 pKa = 9.54 IIKK99 pKa = 9.43 INKK102 pKa = 8.78 IYY104 pKa = 10.67 NPRR107 pKa = 11.84 NMM109 pKa = 4.28
Molecular weight: 12.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.087
IPC2_protein 9.282
IPC_protein 9.165
Toseland 10.218
ProMoST 9.721
Dawson 10.335
Bjellqvist 9.955
Wikipedia 10.423
Rodwell 11.111
Grimsley 10.365
Solomon 10.35
Lehninger 10.335
Nozaki 10.248
DTASelect 9.926
Thurlkill 10.218
EMBOSS 10.599
Sillero 10.262
Patrickios 10.862
IPC_peptide 10.365
IPC2_peptide 8.697
IPC2.peptide.svr19 8.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3813
109
2104
635.5
72.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.436 ± 0.648
1.678 ± 0.318
5.953 ± 0.467
6.216 ± 0.544
3.855 ± 0.287
5.744 ± 0.138
2.229 ± 0.344
8.786 ± 0.717
7.815 ± 0.724
9.31 ± 0.605
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.016 ± 0.245
5.56 ± 0.402
4.878 ± 0.8
3.173 ± 0.262
4.485 ± 0.371
7.763 ± 0.257
5.612 ± 0.485
4.721 ± 0.544
1.836 ± 0.207
3.934 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here