Alteromonas lipolytica
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4316 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E8FGX6|A0A1E8FGX6_9ALTE Lytic transglycosylase OS=Alteromonas lipolytica OX=1856405 GN=BFC17_14120 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.48 LKK4 pKa = 10.36 QLASAVILATSGVAIAPLATAATYY28 pKa = 10.57 DD29 pKa = 3.69 LSLVPVQDD37 pKa = 3.59 KK38 pKa = 11.11 VFLNFAQSIDD48 pKa = 3.58 NTGTMVVTAQGGYY61 pKa = 9.92 NPPIDD66 pKa = 5.18 LSLLDD71 pKa = 5.2 FEE73 pKa = 5.94 DD74 pKa = 3.42 EE75 pKa = 4.15 TFIARR80 pKa = 11.84 FADD83 pKa = 3.91 PEE85 pKa = 4.1 AVEE88 pKa = 3.98 QGIFSSADD96 pKa = 3.36 YY97 pKa = 10.91 QEE99 pKa = 4.71 LYY101 pKa = 10.8 AYY103 pKa = 9.4 IVNARR108 pKa = 11.84 GAGNTTFQPIASYY121 pKa = 10.76 RR122 pKa = 11.84 SYY124 pKa = 11.49 KK125 pKa = 10.31 SDD127 pKa = 3.02 TVTADD132 pKa = 4.7 LIPGFDD138 pKa = 4.15 AFNDD142 pKa = 4.57 AINDD146 pKa = 3.55 YY147 pKa = 9.51 WRR149 pKa = 11.84 QNYY152 pKa = 8.13 TSVYY156 pKa = 9.91 DD157 pKa = 3.99 SVGGDD162 pKa = 3.62 YY163 pKa = 10.83 FVGIANAPFFTVDD176 pKa = 3.26 YY177 pKa = 10.0 TNDD180 pKa = 3.67 DD181 pKa = 3.71 GDD183 pKa = 3.75 EE184 pKa = 3.89 FTYY187 pKa = 10.27 IINNAQTQGFVEE199 pKa = 4.6 VNGNATPLPSALADD213 pKa = 3.67 APIPGYY219 pKa = 9.44 SEE221 pKa = 3.91 PFAINQSLQVVGWASVDD238 pKa = 3.5 VTDD241 pKa = 6.18 DD242 pKa = 3.61 FQEE245 pKa = 5.84 DD246 pKa = 3.07 IDD248 pKa = 4.37 ACLDD252 pKa = 3.52 DD253 pKa = 4.44 EE254 pKa = 4.94 TRR256 pKa = 11.84 GSQPFDD262 pKa = 3.11 VCLYY266 pKa = 10.12 NLSLNVTTGTYY277 pKa = 9.61 RR278 pKa = 11.84 RR279 pKa = 11.84 AAIWQLDD286 pKa = 3.51 AQGNVLSTTPYY297 pKa = 10.97 GMAFEE302 pKa = 5.09 PDD304 pKa = 2.92 EE305 pKa = 4.63 DD306 pKa = 4.53 EE307 pKa = 4.56 YY308 pKa = 10.73 VTGYY312 pKa = 10.51 VSSARR317 pKa = 11.84 DD318 pKa = 3.37 INDD321 pKa = 2.96 NGIAVGYY328 pKa = 8.64 AHH330 pKa = 6.85 NGEE333 pKa = 4.37 FVLYY337 pKa = 8.08 TLPSAQPRR345 pKa = 11.84 RR346 pKa = 11.84 FTFPQEE352 pKa = 3.86 VATTFVDD359 pKa = 4.69 GVVTEE364 pKa = 4.01 ILPRR368 pKa = 11.84 EE369 pKa = 4.29 DD370 pKa = 3.77 SLSSYY375 pKa = 10.55 AQGINNDD382 pKa = 3.26 NWVMGTVLEE391 pKa = 4.54 EE392 pKa = 4.13 NNGFARR398 pKa = 11.84 QHH400 pKa = 6.08 LFVHH404 pKa = 6.15 NLDD407 pKa = 3.63 SGEE410 pKa = 4.59 SIFPQGFFQTAGVRR424 pKa = 11.84 PRR426 pKa = 11.84 AINNNGIVVGEE437 pKa = 4.31 GEE439 pKa = 4.43 YY440 pKa = 10.9 EE441 pKa = 4.11 SDD443 pKa = 3.26 VQYY446 pKa = 11.32 DD447 pKa = 3.64 RR448 pKa = 11.84 QVRR451 pKa = 11.84 AFMYY455 pKa = 9.92 DD456 pKa = 2.92 INVGDD461 pKa = 5.22 FIDD464 pKa = 5.26 LNTLLSCEE472 pKa = 3.74 QRR474 pKa = 11.84 EE475 pKa = 4.3 QYY477 pKa = 10.91 RR478 pKa = 11.84 LVSAADD484 pKa = 3.3 INDD487 pKa = 3.8 DD488 pKa = 4.2 NEE490 pKa = 4.71 IIANALYY497 pKa = 10.7 LDD499 pKa = 3.67 TQRR502 pKa = 11.84 YY503 pKa = 8.14 IDD505 pKa = 4.03 GEE507 pKa = 4.47 EE508 pKa = 4.07 VLTTAGDD515 pKa = 3.76 TIDD518 pKa = 3.46 ANRR521 pKa = 11.84 IVAVKK526 pKa = 8.13 LTPNGTGSVEE536 pKa = 3.93 QCDD539 pKa = 3.32 AAEE542 pKa = 4.01 EE543 pKa = 4.0 QNYY546 pKa = 7.9 EE547 pKa = 4.12 RR548 pKa = 11.84 KK549 pKa = 10.04 GATGTLYY556 pKa = 11.24 GLLTLGLFAFLRR568 pKa = 11.84 RR569 pKa = 11.84 LSFRR573 pKa = 3.77
Molecular weight: 62.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.579
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.012
Patrickios 1.049
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A0A1E8FG89|A0A1E8FG89_9ALTE Sigma-54-dependent Fis family transcriptional regulator OS=Alteromonas lipolytica OX=1856405 GN=BFC17_13510 PE=4 SV=1
MM1 pKa = 7.82 ASTIVLPPATVSRR14 pKa = 11.84 IIEE17 pKa = 4.3 MAWEE21 pKa = 4.07 DD22 pKa = 3.71 RR23 pKa = 11.84 TPFEE27 pKa = 4.98 AIEE30 pKa = 4.07 RR31 pKa = 11.84 EE32 pKa = 4.2 YY33 pKa = 11.41 GLSQDD38 pKa = 3.34 GVIRR42 pKa = 11.84 LMRR45 pKa = 11.84 RR46 pKa = 11.84 EE47 pKa = 4.36 LKK49 pKa = 10.4 PSSFRR54 pKa = 11.84 LWRR57 pKa = 11.84 KK58 pKa = 7.14 RR59 pKa = 11.84 TQGRR63 pKa = 11.84 TTKK66 pKa = 10.04 HH67 pKa = 5.59 LKK69 pKa = 10.05 LRR71 pKa = 11.84 SPDD74 pKa = 2.99 ISRR77 pKa = 11.84 GYY79 pKa = 10.85 CPTQYY84 pKa = 10.69 KK85 pKa = 9.43 QRR87 pKa = 3.81
Molecular weight: 10.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.736
IPC_protein 10.701
Toseland 10.789
ProMoST 10.935
Dawson 10.877
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 10.994
Grimsley 10.921
Solomon 11.052
Lehninger 11.008
Nozaki 10.774
DTASelect 10.628
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.818
Patrickios 10.76
IPC_peptide 11.052
IPC2_peptide 9.692
IPC2.peptide.svr19 8.704
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4316
0
4316
1473345
38
2096
341.4
37.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.577 ± 0.034
1.005 ± 0.013
5.683 ± 0.032
6.029 ± 0.036
4.133 ± 0.025
6.957 ± 0.036
2.16 ± 0.018
5.95 ± 0.026
4.567 ± 0.032
10.321 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.512 ± 0.017
4.096 ± 0.026
4.154 ± 0.022
4.69 ± 0.032
4.679 ± 0.029
6.45 ± 0.026
5.586 ± 0.031
7.018 ± 0.028
1.277 ± 0.016
3.155 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here