Mycobacterium phage MOOREtheMARYer

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6SJW4|A0A0F6SJW4_9CAUD Uncharacterized protein OS=Mycobacterium phage MOOREtheMARYer OX=1647309 GN=61 PE=4 SV=1
MM1 pKa = 7.81IEE3 pKa = 4.46PDD5 pKa = 3.16LTGRR9 pKa = 11.84CLLCDD14 pKa = 3.98AGLGGHH20 pKa = 7.21FIDD23 pKa = 5.74CPGVLDD29 pKa = 3.89AAEE32 pKa = 4.48PPRR35 pKa = 11.84PEE37 pKa = 4.57PVDD40 pKa = 3.71LDD42 pKa = 3.88EE43 pKa = 5.27PKK45 pKa = 10.77EE46 pKa = 4.15SS47 pKa = 3.54

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6WEQ9|A0A0F6WEQ9_9CAUD Uncharacterized protein OS=Mycobacterium phage MOOREtheMARYer OX=1647309 GN=54 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84PLYY5 pKa = 10.36VRR7 pKa = 11.84QSEE10 pKa = 4.37AGPNASNTGDD20 pKa = 3.05YY21 pKa = 9.62WYY23 pKa = 9.41TVSTGNGEE31 pKa = 4.69PILTSKK37 pKa = 10.07MYY39 pKa = 8.87RR40 pKa = 11.84TRR42 pKa = 11.84ARR44 pKa = 11.84AIRR47 pKa = 11.84AARR50 pKa = 11.84AFMRR54 pKa = 11.84RR55 pKa = 11.84TRR57 pKa = 11.84GPVTFSYY64 pKa = 9.34WAGYY68 pKa = 8.29TPTQQAEE75 pKa = 3.84RR76 pKa = 11.84MAIGRR81 pKa = 11.84QPRR84 pKa = 11.84GQLQLVTEE92 pKa = 5.29HH93 pKa = 6.19YY94 pKa = 10.71QFADD98 pKa = 3.06

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14583

47

1336

217.7

23.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.125 ± 0.613

0.885 ± 0.153

7.221 ± 0.318

5.554 ± 0.401

2.976 ± 0.172

9.415 ± 0.848

1.989 ± 0.198

4.252 ± 0.208

3.415 ± 0.27

7.399 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.177

3.086 ± 0.191

6.309 ± 0.381

3.586 ± 0.164

7.2 ± 0.485

4.498 ± 0.256

6.295 ± 0.302

6.542 ± 0.26

1.968 ± 0.151

2.139 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski