Alces alces faeces associated genomovirus MP84
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CK60|A0A2Z5CK60_9VIRU Replication-associated protein OS=Alces alces faeces associated genomovirus MP84 OX=2219116 PE=3 SV=1
MM1 pKa = 7.88 PRR3 pKa = 11.84 FLCNASHH10 pKa = 7.43 FLITYY15 pKa = 7.23 AQCGDD20 pKa = 3.42 LSEE23 pKa = 4.27 WRR25 pKa = 11.84 VLDD28 pKa = 4.01 CFTLLGAQCIIARR41 pKa = 11.84 EE42 pKa = 3.97 YY43 pKa = 11.19 HH44 pKa = 6.63 EE45 pKa = 5.57 DD46 pKa = 3.53 LGIHH50 pKa = 5.87 LHH52 pKa = 5.56 VFVDD56 pKa = 4.71 FRR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 8.85 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 VDD67 pKa = 2.84 IFDD70 pKa = 3.17 VDD72 pKa = 3.57 GRR74 pKa = 11.84 HH75 pKa = 6.05 PNIKK79 pKa = 9.98 RR80 pKa = 11.84 SWGTPEE86 pKa = 4.27 KK87 pKa = 10.76 GYY89 pKa = 10.97 DD90 pKa = 3.47 YY91 pKa = 10.69 AIKK94 pKa = 10.87 DD95 pKa = 3.59 GDD97 pKa = 4.01 VVAGGLEE104 pKa = 4.18 RR105 pKa = 11.84 PDD107 pKa = 3.36 GTFGSGKK114 pKa = 9.79 NFDD117 pKa = 4.27 LWSQIAGAGSKK128 pKa = 11.05 AEE130 pKa = 4.04 FWDD133 pKa = 3.76 LCEE136 pKa = 4.22 EE137 pKa = 4.73 LDD139 pKa = 4.84 PKK141 pKa = 11.48 SMWCSFGQLQKK152 pKa = 10.65 FADD155 pKa = 3.31 WRR157 pKa = 11.84 FAEE160 pKa = 4.67 VPTEE164 pKa = 3.85 YY165 pKa = 10.89 ANPGGFEE172 pKa = 4.08 FVDD175 pKa = 4.28 GNDD178 pKa = 4.25 DD179 pKa = 3.56 GRR181 pKa = 11.84 TAPMSLVLYY190 pKa = 10.49 GEE192 pKa = 4.26 SRR194 pKa = 11.84 TGKK197 pKa = 7.35 TLWARR202 pKa = 11.84 SLGPHH207 pKa = 7.22 IYY209 pKa = 10.46 NVGLVSGEE217 pKa = 4.0 EE218 pKa = 4.6 CSRR221 pKa = 11.84 APHH224 pKa = 5.21 VKK226 pKa = 10.21 YY227 pKa = 10.68 AVFDD231 pKa = 4.9 DD232 pKa = 3.28 IRR234 pKa = 11.84 GGIKK238 pKa = 9.97 FFPAFKK244 pKa = 9.79 EE245 pKa = 4.03 WLGGQSTVCVKK256 pKa = 10.49 RR257 pKa = 11.84 LYY259 pKa = 10.38 RR260 pKa = 11.84 DD261 pKa = 3.5 PKK263 pKa = 10.22 LVTWGKK269 pKa = 9.15 PSIWVSNDD277 pKa = 3.26 DD278 pKa = 4.22 PRR280 pKa = 11.84 HH281 pKa = 5.73 AMDD284 pKa = 5.22 PSDD287 pKa = 4.5 VSWLEE292 pKa = 3.46 ANARR296 pKa = 11.84 FIEE299 pKa = 3.97 ITEE302 pKa = 4.67 PIFRR306 pKa = 11.84 ANTGG310 pKa = 3.1
Molecular weight: 35.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.569
IPC2_protein 5.55
IPC_protein 5.55
Toseland 5.842
ProMoST 5.868
Dawson 5.753
Bjellqvist 5.792
Wikipedia 5.741
Rodwell 5.728
Grimsley 5.944
Solomon 5.753
Lehninger 5.741
Nozaki 5.995
DTASelect 6.186
Thurlkill 6.135
EMBOSS 6.109
Sillero 6.097
Patrickios 4.164
IPC_peptide 5.779
IPC2_peptide 6.097
IPC2.peptide.svr19 6.09
Protein with the highest isoelectric point:
>tr|A0A2Z5CK60|A0A2Z5CK60_9VIRU Replication-associated protein OS=Alces alces faeces associated genomovirus MP84 OX=2219116 PE=3 SV=1
MM1 pKa = 8.1 AYY3 pKa = 10.29 RR4 pKa = 11.84 STKK7 pKa = 9.25 RR8 pKa = 11.84 RR9 pKa = 11.84 SPVRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 8.77 PARR19 pKa = 11.84 KK20 pKa = 8.78 YY21 pKa = 10.63 GGRR24 pKa = 11.84 TTRR27 pKa = 11.84 RR28 pKa = 11.84 SYY30 pKa = 11.03 AKK32 pKa = 10.03 KK33 pKa = 8.36 RR34 pKa = 11.84 TYY36 pKa = 10.13 RR37 pKa = 11.84 KK38 pKa = 9.41 KK39 pKa = 10.78 ASSKK43 pKa = 9.65 MSNKK47 pKa = 10.07 RR48 pKa = 11.84 ILNLTSIKK56 pKa = 10.5 KK57 pKa = 9.84 RR58 pKa = 11.84 DD59 pKa = 3.76 TMLSAYY65 pKa = 10.05 QLGSTVNPQPGAYY78 pKa = 8.42 PVQGTGGGNGGNTTVLLWCATARR101 pKa = 11.84 DD102 pKa = 3.84 NTVISGGGSGNRR114 pKa = 11.84 FTPATRR120 pKa = 11.84 TATTCFMRR128 pKa = 11.84 GLAEE132 pKa = 4.03 NVEE135 pKa = 4.47 LSTNTGVAWQWRR147 pKa = 11.84 RR148 pKa = 11.84 ICFTFKK154 pKa = 11.11 DD155 pKa = 4.2 PGALSQTQTYY165 pKa = 9.9 FLEE168 pKa = 4.71 TSSGFQRR175 pKa = 11.84 NNYY178 pKa = 9.01 NVTSGSSPDD187 pKa = 3.44 STNLEE192 pKa = 4.09 NLRR195 pKa = 11.84 QLVFRR200 pKa = 11.84 GQLGTDD206 pKa = 2.82 WSSYY210 pKa = 7.89 LTAPVDD216 pKa = 3.92 NSRR219 pKa = 11.84 CTIKK223 pKa = 10.57 YY224 pKa = 10.32 DD225 pKa = 2.84 KK226 pKa = 8.17 TTNVNSGNANGKK238 pKa = 9.0 LVNRR242 pKa = 11.84 KK243 pKa = 8.13 LWMPMNSNLVYY254 pKa = 10.84 DD255 pKa = 4.53 DD256 pKa = 5.43 DD257 pKa = 4.34 EE258 pKa = 6.52 LGGAEE263 pKa = 4.05 AARR266 pKa = 11.84 PYY268 pKa = 10.82 SIGGKK273 pKa = 9.45 QGMGDD278 pKa = 4.24 YY279 pKa = 10.98 YY280 pKa = 11.77 VMDD283 pKa = 3.99 IFQAGVGATSSDD295 pKa = 3.62 LLSFLPQATLYY306 pKa = 9.14 WHH308 pKa = 6.98 EE309 pKa = 4.28 KK310 pKa = 9.3
Molecular weight: 34.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.287
IPC2_protein 9.663
IPC_protein 10.058
Toseland 10.131
ProMoST 9.926
Dawson 10.365
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.657
Grimsley 10.452
Solomon 10.394
Lehninger 10.35
Nozaki 10.131
DTASelect 10.072
Thurlkill 10.204
EMBOSS 10.54
Sillero 10.277
Patrickios 10.058
IPC_peptide 10.379
IPC2_peptide 8.975
IPC2.peptide.svr19 8.595
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
620
310
310
310.0
34.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.774 ± 0.0
1.935 ± 0.482
6.129 ± 1.446
4.032 ± 1.326
4.839 ± 1.446
9.839 ± 0.121
1.452 ± 0.844
3.548 ± 0.964
5.161 ± 0.241
7.258 ± 0.121
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.935 ± 0.482
5.0 ± 1.567
4.516 ± 0.482
2.903 ± 0.723
7.742 ± 0.482
7.581 ± 1.085
7.097 ± 2.411
5.484 ± 0.482
2.742 ± 0.603
4.032 ± 0.844
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here