Natronomonas salsuginis
Average proteome isoelectric point is 4.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2818 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V5ZPF7|A0A4V5ZPF7_9EURY Vitamin B12-dependent ribonucleotide reductase OS=Natronomonas salsuginis OX=2217661 GN=DM868_00080 PE=3 SV=1
MM1 pKa = 7.4 GLDD4 pKa = 4.06 EE5 pKa = 6.05 LSDD8 pKa = 5.63 DD9 pKa = 4.76 IDD11 pKa = 3.3 ATYY14 pKa = 11.53 ADD16 pKa = 4.59 LGEE19 pKa = 4.43 EE20 pKa = 4.24 LSVALDD26 pKa = 3.49 TEE28 pKa = 4.65 TRR30 pKa = 11.84 NEE32 pKa = 3.83 LAMLEE37 pKa = 4.36 SVYY40 pKa = 11.16 DD41 pKa = 3.8 PDD43 pKa = 3.75 EE44 pKa = 4.08 TDD46 pKa = 3.12 EE47 pKa = 4.08 LVRR50 pKa = 11.84 RR51 pKa = 11.84 AIHH54 pKa = 6.04 MLFQTAVDD62 pKa = 3.55 TGNLDD67 pKa = 3.1 FHH69 pKa = 6.97 LRR71 pKa = 11.84 SGYY74 pKa = 10.69 DD75 pKa = 3.15 VTYY78 pKa = 10.86 DD79 pKa = 3.54 EE80 pKa = 5.33 YY81 pKa = 11.78 LMGMTMDD88 pKa = 4.75 TYY90 pKa = 11.43 NEE92 pKa = 4.04 QPQQQQYY99 pKa = 9.9 PPQDD103 pKa = 3.55 DD104 pKa = 3.4 NARR107 pKa = 11.84 RR108 pKa = 11.84 YY109 pKa = 8.28 QFF111 pKa = 3.67
Molecular weight: 12.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.49
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A4V5ZND3|A0A4V5ZND3_9EURY ATP-synt_C domain-containing protein OS=Natronomonas salsuginis OX=2217661 GN=DM868_12805 PE=4 SV=1
MM1 pKa = 7.5 EE2 pKa = 4.17 MPRR5 pKa = 11.84 RR6 pKa = 11.84 FNTYY10 pKa = 9.29 CPHH13 pKa = 6.64 CHH15 pKa = 5.33 THH17 pKa = 5.89 HH18 pKa = 5.09 QHH20 pKa = 5.32 EE21 pKa = 4.65 VEE23 pKa = 4.23 KK24 pKa = 10.74 VRR26 pKa = 11.84 SGRR29 pKa = 11.84 SSGTKK34 pKa = 8.02 WDD36 pKa = 3.19 ARR38 pKa = 11.84 RR39 pKa = 11.84 TRR41 pKa = 11.84 RR42 pKa = 11.84 GKK44 pKa = 10.51 AVIGNAGRR52 pKa = 11.84 FSKK55 pKa = 11.05 VPGGDD60 pKa = 3.29 KK61 pKa = 9.11 PTKK64 pKa = 9.73 KK65 pKa = 8.97 TDD67 pKa = 3.09 LKK69 pKa = 10.75 YY70 pKa = 10.63 RR71 pKa = 11.84 CSEE74 pKa = 3.99 CGKK77 pKa = 10.02 AHH79 pKa = 7.52 LRR81 pKa = 11.84 QGWRR85 pKa = 11.84 AGRR88 pKa = 11.84 LIFQEE93 pKa = 4.13
Molecular weight: 10.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.633
IPC_protein 10.204
Toseland 10.818
ProMoST 10.438
Dawson 10.891
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.184
Grimsley 10.906
Solomon 11.008
Lehninger 10.979
Nozaki 10.818
DTASelect 10.555
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.818
Patrickios 10.921
IPC_peptide 11.023
IPC2_peptide 9.692
IPC2.peptide.svr19 8.642
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2818
0
2818
826435
30
9006
293.3
31.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.711 ± 0.061
0.739 ± 0.014
8.143 ± 0.056
8.573 ± 0.079
3.354 ± 0.033
8.697 ± 0.082
1.999 ± 0.024
4.756 ± 0.04
1.971 ± 0.031
8.777 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.797 ± 0.022
2.437 ± 0.039
4.576 ± 0.031
2.256 ± 0.025
6.727 ± 0.054
5.589 ± 0.06
6.389 ± 0.073
8.768 ± 0.046
1.036 ± 0.016
2.705 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here