Klebsiella phage VLC4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9IBF0|A0A6B9IBF0_9CAUD DNA helicase OS=Klebsiella phage VLC4 OX=2686207 PE=4 SV=1
MM1 pKa = 7.26VNVFNIIVTSAMLVLGNDD19 pKa = 3.45VNNPVPYY26 pKa = 9.36CTVQLQQPATQEE38 pKa = 3.83PQPRR42 pKa = 11.84PEE44 pKa = 3.92YY45 pKa = 10.91DD46 pKa = 3.21LFEE49 pKa = 6.24DD50 pKa = 4.38PEE52 pKa = 4.87GGCKK56 pKa = 9.76EE57 pKa = 3.82LGARR61 pKa = 11.84ILAAVQEE68 pKa = 4.52QYY70 pKa = 11.29PDD72 pKa = 3.32AAVTLTVDD80 pKa = 4.21GKK82 pKa = 11.1SSNDD86 pKa = 2.86II87 pKa = 3.8

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9I9Q9|A0A6B9I9Q9_9CAUD DNA-directed RNA polymerase OS=Klebsiella phage VLC4 OX=2686207 PE=3 SV=1
MM1 pKa = 8.21RR2 pKa = 11.84IITWAKK8 pKa = 7.54EE9 pKa = 3.66QYY11 pKa = 11.04AVFLLLRR18 pKa = 11.84AQRR21 pKa = 11.84LQKK24 pKa = 10.4RR25 pKa = 11.84ANDD28 pKa = 3.44WHH30 pKa = 6.22WAANSHH36 pKa = 5.64AHH38 pKa = 6.45RR39 pKa = 11.84ASHH42 pKa = 6.96LGNEE46 pKa = 4.44ISAHH50 pKa = 6.35RR51 pKa = 11.84YY52 pKa = 8.79HH53 pKa = 7.31LTRR56 pKa = 11.84QCAKK60 pKa = 9.58SRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84AYY66 pKa = 10.62ALGAEE71 pKa = 4.45ATATEE76 pKa = 4.55TKK78 pKa = 10.23AHH80 pKa = 5.85NFISKK85 pKa = 10.22HH86 pKa = 4.86KK87 pKa = 10.71LKK89 pKa = 11.07GFDD92 pKa = 3.17

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

14055

31

1232

226.7

25.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.494 ± 0.569

1.266 ± 0.173

6.211 ± 0.221

5.87 ± 0.333

3.024 ± 0.146

7.442 ± 0.266

1.985 ± 0.186

4.468 ± 0.171

4.867 ± 0.283

8.758 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.236

3.963 ± 0.217

3.735 ± 0.257

4.511 ± 0.304

6.041 ± 0.267

6.467 ± 0.367

5.599 ± 0.272

7.065 ± 0.248

1.487 ± 0.101

3.899 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski