Amino acid dipepetide frequency for Crocuta crocuta (Spotted hyena)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.336AlaAla: 6.336 ± 0.056	1.441AlaCys: 1.441 ± 0.016	2.823AlaAsp: 2.823 ± 0.024	4.643AlaGlu: 4.643 ± 0.037	2.691AlaPhe: 2.691 ± 0.026	4.551AlaGly: 4.551 ± 0.038	1.541AlaHis: 1.541 ± 0.016	2.983AlaIle: 2.983 ± 0.026	3.579AlaLys: 3.579 ± 0.03	7.323AlaLeu: 7.323 ± 0.045	1.551AlaMet: 1.551 ± 0.015	2.007AlaAsn: 2.007 ± 0.016	4.017AlaPro: 4.017 ± 0.046	3.081AlaGln: 3.081 ± 0.03	3.672AlaArg: 3.672 ± 0.03	5.553AlaSer: 5.553 ± 0.036	3.559AlaThr: 3.559 ± 0.023	4.664AlaVal: 4.664 ± 0.031	0.791AlaTrp: 0.791 ± 0.011	1.551AlaTyr: 1.551 ± 0.015	0.013AlaXaa: 0.013 ± 0.001
Cys	1.304CysAla: 1.304 ± 0.015	0.624CysCys: 0.624 ± 0.015	1.025CysAsp: 1.025 ± 0.016	1.247CysGlu: 1.247 ± 0.018	0.897CysPhe: 0.897 ± 0.012	1.849CysGly: 1.849 ± 0.03	0.662CysHis: 0.662 ± 0.012	0.985CysIle: 0.985 ± 0.013	1.244CysLys: 1.244 ± 0.017	2.175CysLeu: 2.175 ± 0.022	0.404CysMet: 0.404 ± 0.008	0.814CysAsn: 0.814 ± 0.014	1.556CysPro: 1.556 ± 0.024	1.025CysGln: 1.025 ± 0.019	1.316CysArg: 1.316 ± 0.019	2.049CysSer: 2.049 ± 0.024	1.249CysThr: 1.249 ± 0.017	1.474CysVal: 1.474 ± 0.018	0.49CysTrp: 0.49 ± 0.011	0.603CysTyr: 0.603 ± 0.008	0.003CysXaa: 0.003 ± 0.001
Asp	2.744AspAla: 2.744 ± 0.021	1.017AspCys: 1.017 ± 0.014	2.45AspAsp: 2.45 ± 0.024	3.382AspGlu: 3.382 ± 0.025	2.1AspPhe: 2.1 ± 0.019	3.132AspGly: 3.132 ± 0.033	1.127AspHis: 1.127 ± 0.012	2.671AspIle: 2.671 ± 0.025	2.579AspLys: 2.579 ± 0.022	5.006AspLeu: 5.006 ± 0.029	1.081AspMet: 1.081 ± 0.012	1.601AspAsn: 1.601 ± 0.019	2.966AspPro: 2.966 ± 0.026	1.759AspGln: 1.759 ± 0.016	2.445AspArg: 2.445 ± 0.022	3.938AspSer: 3.938 ± 0.03	2.564AspThr: 2.564 ± 0.018	2.972AspVal: 2.972 ± 0.037	0.631AspTrp: 0.631 ± 0.01	1.422AspTyr: 1.422 ± 0.015	0.004AspXaa: 0.004 ± 0.001
Glu	5.031GluAla: 5.031 ± 0.037	1.543GluCys: 1.543 ± 0.037	4.234GluAsp: 4.234 ± 0.029	7.428GluGlu: 7.428 ± 0.073	2.041GluPhe: 2.041 ± 0.017	4.235GluGly: 4.235 ± 0.032	1.471GluHis: 1.471 ± 0.015	3.423GluIle: 3.423 ± 0.027	5.338GluLys: 5.338 ± 0.047	6.345GluLeu: 6.345 ± 0.046	1.661GluMet: 1.661 ± 0.016	3.088GluAsn: 3.088 ± 0.023	3.187GluPro: 3.187 ± 0.032	2.938GluGln: 2.938 ± 0.027	3.816GluArg: 3.816 ± 0.033	4.127GluSer: 4.127 ± 0.031	3.363GluThr: 3.363 ± 0.022	4.051GluVal: 4.051 ± 0.024	0.85GluTrp: 0.85 ± 0.011	1.671GluTyr: 1.671 ± 0.02	0.006GluXaa: 0.006 ± 0.001
Phe	2.103PheAla: 2.103 ± 0.017	1.037PheCys: 1.037 ± 0.014	1.658PheAsp: 1.658 ± 0.017	1.95PheGlu: 1.95 ± 0.018	1.82PhePhe: 1.82 ± 0.022	2.204PheGly: 2.204 ± 0.022	1.05PheHis: 1.05 ± 0.012	2.244PheIle: 2.244 ± 0.023	1.763PheLys: 1.763 ± 0.018	4.521PheLeu: 4.521 ± 0.033	0.748PheMet: 0.748 ± 0.011	1.359PheAsn: 1.359 ± 0.015	2.046PhePro: 2.046 ± 0.02	1.79PheGln: 1.79 ± 0.016	1.959PheArg: 1.959 ± 0.022	3.563PheSer: 3.563 ± 0.029	2.092PheThr: 2.092 ± 0.019	2.255PheVal: 2.255 ± 0.019	0.522PheTrp: 0.522 ± 0.008	1.272PheTyr: 1.272 ± 0.014	0.003PheXaa: 0.003 ± 0.001
Gly	4.393GlyAla: 4.393 ± 0.04	1.384GlyCys: 1.384 ± 0.019	3.037GlyAsp: 3.037 ± 0.032	4.068GlyGlu: 4.068 ± 0.04	2.422GlyPhe: 2.422 ± 0.024	4.724GlyGly: 4.724 ± 0.044	1.657GlyHis: 1.657 ± 0.019	2.953GlyIle: 2.953 ± 0.025	3.752GlyLys: 3.752 ± 0.037	5.96GlyLeu: 5.96 ± 0.039	1.241GlyMet: 1.241 ± 0.015	2.353GlyAsn: 2.353 ± 0.022	4.184GlyPro: 4.184 ± 0.084	2.68GlyGln: 2.68 ± 0.033	3.628GlyArg: 3.628 ± 0.029	5.421GlySer: 5.421 ± 0.043	3.593GlyThr: 3.593 ± 0.027	3.693GlyVal: 3.693 ± 0.03	0.779GlyTrp: 0.779 ± 0.011	1.685GlyTyr: 1.685 ± 0.023	0.018GlyXaa: 0.018 ± 0.002
His	1.332HisAla: 1.332 ± 0.014	0.813HisCys: 0.813 ± 0.013	0.855HisAsp: 0.855 ± 0.011	1.261HisGlu: 1.261 ± 0.015	1.173HisPhe: 1.173 ± 0.013	1.517HisGly: 1.517 ± 0.018	0.853HisHis: 0.853 ± 0.014	1.284HisIle: 1.284 ± 0.012	1.224HisLys: 1.224 ± 0.013	2.985HisLeu: 2.985 ± 0.025	0.714HisMet: 0.714 ± 0.01	0.842HisAsn: 0.842 ± 0.01	1.708HisPro: 1.708 ± 0.027	1.292HisGln: 1.292 ± 0.019	1.714HisArg: 1.714 ± 0.019	2.196HisSer: 2.196 ± 0.023	1.581HisThr: 1.581 ± 0.028	1.496HisVal: 1.496 ± 0.016	0.343HisTrp: 0.343 ± 0.007	0.823HisTyr: 0.823 ± 0.01	0.004HisXaa: 0.004 ± 0.001
Ile	3.008IleAla: 3.008 ± 0.025	1.16IleCys: 1.16 ± 0.014	1.982IleAsp: 1.982 ± 0.021	2.656IleGlu: 2.656 ± 0.022	2.014IlePhe: 2.014 ± 0.022	2.339IleGly: 2.339 ± 0.021	1.481IleHis: 1.481 ± 0.029	2.534IleIle: 2.534 ± 0.026	2.89IleLys: 2.89 ± 0.026	4.79IleLeu: 4.79 ± 0.034	1.003IleMet: 1.003 ± 0.014	1.859IleAsn: 1.859 ± 0.018	2.597IlePro: 2.597 ± 0.02	2.367IleGln: 2.367 ± 0.022	2.458IleArg: 2.458 ± 0.02	3.897IleSer: 3.897 ± 0.025	2.593IleThr: 2.593 ± 0.024	2.577IleVal: 2.577 ± 0.024	0.671IleTrp: 0.671 ± 0.012	1.808IleTyr: 1.808 ± 0.019	0.003IleXaa: 0.003 ± 0.001
Lys	4.003LysAla: 4.003 ± 0.035	1.247LysCys: 1.247 ± 0.018	3.162LysAsp: 3.162 ± 0.027	5.214LysGlu: 5.214 ± 0.046	1.725LysPhe: 1.725 ± 0.018	3.234LysGly: 3.234 ± 0.044	1.36LysHis: 1.36 ± 0.015	2.895LysIle: 2.895 ± 0.025	4.825LysLys: 4.825 ± 0.049	5.321LysLeu: 5.321 ± 0.039	1.626LysMet: 1.626 ± 0.022	2.485LysAsn: 2.485 ± 0.024	2.996LysPro: 2.996 ± 0.031	2.487LysGln: 2.487 ± 0.024	3.237LysArg: 3.237 ± 0.03	3.853LysSer: 3.853 ± 0.032	3.421LysThr: 3.421 ± 0.026	3.403LysVal: 3.403 ± 0.024	0.667LysTrp: 0.667 ± 0.011	1.648LysTyr: 1.648 ± 0.017	0.006LysXaa: 0.006 ± 0.001
Leu	6.776LeuAla: 6.776 ± 0.041	2.213LeuCys: 2.213 ± 0.025	4.609LeuAsp: 4.609 ± 0.027	7.028LeuGlu: 7.028 ± 0.046	3.583LeuPhe: 3.583 ± 0.03	6.055LeuGly: 6.055 ± 0.034	2.862LeuHis: 2.862 ± 0.023	4.212LeuIle: 4.212 ± 0.028	5.754LeuLys: 5.754 ± 0.038	11.068LeuLeu: 11.068 ± 0.066	2.067LeuMet: 2.067 ± 0.021	3.481LeuAsn: 3.481 ± 0.026	5.956LeuPro: 5.956 ± 0.045	5.474LeuGln: 5.474 ± 0.044	6.0LeuArg: 6.0 ± 0.041	8.258LeuSer: 8.258 ± 0.045	5.359LeuThr: 5.359 ± 0.03	5.679LeuVal: 5.679 ± 0.036	1.271LeuTrp: 1.271 ± 0.015	2.607LeuTyr: 2.607 ± 0.02	0.011LeuXaa: 0.011 ± 0.001
Met	1.951MetAla: 1.951 ± 0.018	0.404MetCys: 0.404 ± 0.008	1.272MetAsp: 1.272 ± 0.014	1.881MetGlu: 1.881 ± 0.018	1.006MetPhe: 1.006 ± 0.014	1.256MetGly: 1.256 ± 0.034	0.543MetHis: 0.543 ± 0.009	0.849MetIle: 0.849 ± 0.012	1.458MetLys: 1.458 ± 0.017	2.146MetLeu: 2.146 ± 0.02	0.514MetMet: 0.514 ± 0.011	0.904MetAsn: 0.904 ± 0.012	1.008MetPro: 1.008 ± 0.02	0.844MetGln: 0.844 ± 0.012	1.109MetArg: 1.109 ± 0.012	1.521MetSer: 1.521 ± 0.017	1.086MetThr: 1.086 ± 0.013	1.345MetVal: 1.345 ± 0.015	0.377MetTrp: 0.377 ± 0.008	0.66MetTyr: 0.66 ± 0.01	0.001MetXaa: 0.001 ± 0.0
Asn	1.984AsnAla: 1.984 ± 0.019	0.839AsnCys: 0.839 ± 0.011	1.488AsnAsp: 1.488 ± 0.018	2.302AsnGlu: 2.302 ± 0.022	1.513AsnPhe: 1.513 ± 0.018	2.302AsnGly: 2.302 ± 0.025	0.938AsnHis: 0.938 ± 0.013	2.148AsnIle: 2.148 ± 0.02	2.208AsnLys: 2.208 ± 0.022	3.701AsnLeu: 3.701 ± 0.028	0.834AsnMet: 0.834 ± 0.012	1.627AsnAsn: 1.627 ± 0.017	2.181AsnPro: 2.181 ± 0.019	1.576AsnGln: 1.576 ± 0.017	1.93AsnArg: 1.93 ± 0.017	3.046AsnSer: 3.046 ± 0.026	1.916AsnThr: 1.916 ± 0.018	2.183AsnVal: 2.183 ± 0.019	0.454AsnTrp: 0.454 ± 0.008	1.087AsnTyr: 1.087 ± 0.015	0.004AsnXaa: 0.004 ± 0.001
Pro	4.715ProAla: 4.715 ± 0.048	1.278ProCys: 1.278 ± 0.021	2.715ProAsp: 2.715 ± 0.028	4.328ProGlu: 4.328 ± 0.031	2.008ProPhe: 2.008 ± 0.019	5.081ProGly: 5.081 ± 0.109	1.407ProHis: 1.407 ± 0.022	1.839ProIle: 1.839 ± 0.018	2.943ProLys: 2.943 ± 0.041	5.346ProLeu: 5.346 ± 0.035	1.162ProMet: 1.162 ± 0.014	1.758ProAsn: 1.758 ± 0.019	5.533ProPro: 5.533 ± 0.083	2.738ProGln: 2.738 ± 0.044	3.37ProArg: 3.37 ± 0.028	5.593ProSer: 5.593 ± 0.051	2.921ProThr: 2.921 ± 0.029	3.795ProVal: 3.795 ± 0.029	0.692ProTrp: 0.692 ± 0.011	1.619ProTyr: 1.619 ± 0.026	0.015ProXaa: 0.015 ± 0.001
Gln	3.419GlnAla: 3.419 ± 0.03	0.919GlnCys: 0.919 ± 0.016	2.218GlnAsp: 2.218 ± 0.018	3.724GlnGlu: 3.724 ± 0.035	1.315GlnPhe: 1.315 ± 0.014	2.762GlnGly: 2.762 ± 0.03	1.231GlnHis: 1.231 ± 0.018	2.025GlnIle: 2.025 ± 0.018	2.871GlnLys: 2.871 ± 0.026	4.514GlnLeu: 4.514 ± 0.033	1.097GlnMet: 1.097 ± 0.013	1.78GlnAsn: 1.78 ± 0.017	2.742GlnPro: 2.742 ± 0.03	2.67GlnGln: 2.67 ± 0.041	2.851GlnArg: 2.851 ± 0.027	2.93GlnSer: 2.93 ± 0.031	2.244GlnThr: 2.244 ± 0.035	2.777GlnVal: 2.777 ± 0.024	0.545GlnTrp: 0.545 ± 0.01	1.087GlnTyr: 1.087 ± 0.013	0.006GlnXaa: 0.006 ± 0.001
Arg	3.794ArgAla: 3.794 ± 0.032	1.333ArgCys: 1.333 ± 0.02	2.787ArgAsp: 2.787 ± 0.026	3.924ArgGlu: 3.924 ± 0.035	1.958ArgPhe: 1.958 ± 0.017	3.659ArgGly: 3.659 ± 0.039	1.628ArgHis: 1.628 ± 0.018	2.539ArgIle: 2.539 ± 0.02	3.761ArgLys: 3.761 ± 0.029	5.219ArgLeu: 5.219 ± 0.036	1.158ArgMet: 1.158 ± 0.017	2.153ArgAsn: 2.153 ± 0.018	3.15ArgPro: 3.15 ± 0.035	2.518ArgGln: 2.518 ± 0.022	4.064ArgArg: 4.064 ± 0.035	4.124ArgSer: 4.124 ± 0.04	2.747ArgThr: 2.747 ± 0.025	3.194ArgVal: 3.194 ± 0.028	0.694ArgTrp: 0.694 ± 0.01	1.613ArgTyr: 1.613 ± 0.015	0.013ArgXaa: 0.013 ± 0.001
Ser	5.067SerAla: 5.067 ± 0.028	1.821SerCys: 1.821 ± 0.024	3.743SerAsp: 3.743 ± 0.03	5.065SerGlu: 5.065 ± 0.034	3.129SerPhe: 3.129 ± 0.023	5.393SerGly: 5.393 ± 0.045	2.073SerHis: 2.073 ± 0.022	3.237SerIle: 3.237 ± 0.032	4.035SerLys: 4.035 ± 0.031	8.148SerLeu: 8.148 ± 0.045	1.471SerMet: 1.471 ± 0.014	2.569SerAsn: 2.569 ± 0.021	5.693SerPro: 5.693 ± 0.049	3.775SerGln: 3.775 ± 0.03	4.42SerArg: 4.42 ± 0.037	8.51SerSer: 8.51 ± 0.074	4.587SerThr: 4.587 ± 0.029	4.793SerVal: 4.793 ± 0.026	1.072SerTrp: 1.072 ± 0.015	2.115SerTyr: 2.115 ± 0.021	0.012SerXaa: 0.012 ± 0.001
Thr	3.666ThrAla: 3.666 ± 0.028	1.474ThrCys: 1.474 ± 0.019	2.552ThrAsp: 2.552 ± 0.019	3.544ThrGlu: 3.544 ± 0.025	2.155ThrPhe: 2.155 ± 0.018	3.572ThrGly: 3.572 ± 0.032	1.322ThrHis: 1.322 ± 0.02	2.728ThrIle: 2.728 ± 0.025	2.622ThrLys: 2.622 ± 0.022	5.418ThrLeu: 5.418 ± 0.033	1.241ThrMet: 1.241 ± 0.034	1.752ThrAsn: 1.752 ± 0.019	3.648ThrPro: 3.648 ± 0.033	2.228ThrGln: 2.228 ± 0.019	2.418ThrArg: 2.418 ± 0.024	4.294ThrSer: 4.294 ± 0.026	2.874ThrThr: 2.874 ± 0.028	4.0ThrVal: 4.0 ± 0.03	0.957ThrTrp: 0.957 ± 0.014	1.429ThrTyr: 1.429 ± 0.015	0.006ThrXaa: 0.006 ± 0.001
Val	4.475ValAla: 4.475 ± 0.026	1.485ValCys: 1.485 ± 0.018	2.884ValAsp: 2.884 ± 0.029	3.642ValGlu: 3.642 ± 0.027	2.487ValPhe: 2.487 ± 0.022	3.35ValGly: 3.35 ± 0.026	1.555ValHis: 1.555 ± 0.02	2.981ValIle: 2.981 ± 0.025	3.348ValLys: 3.348 ± 0.029	6.38ValLeu: 6.38 ± 0.034	1.411ValMet: 1.411 ± 0.014	2.202ValAsn: 2.202 ± 0.021	3.564ValPro: 3.564 ± 0.031	2.746ValGln: 2.746 ± 0.022	3.141ValArg: 3.141 ± 0.025	4.801ValSer: 4.801 ± 0.028	3.772ValThr: 3.772 ± 0.031	3.993ValVal: 3.993 ± 0.029	0.869ValTrp: 0.869 ± 0.011	1.665ValTyr: 1.665 ± 0.015	0.007ValXaa: 0.007 ± 0.001
Trp	0.833TrpAla: 0.833 ± 0.013	0.257TrpCys: 0.257 ± 0.005	0.658TrpAsp: 0.658 ± 0.011	0.921TrpGlu: 0.921 ± 0.013	0.597TrpPhe: 0.597 ± 0.009	0.739TrpGly: 0.739 ± 0.013	0.309TrpHis: 0.309 ± 0.007	0.726TrpIle: 0.726 ± 0.011	1.112TrpLys: 1.112 ± 0.017	1.194TrpLeu: 1.194 ± 0.014	0.436TrpMet: 0.436 ± 0.008	0.538TrpAsn: 0.538 ± 0.008	0.654TrpPro: 0.654 ± 0.009	0.526TrpGln: 0.526 ± 0.009	0.906TrpArg: 0.906 ± 0.012	0.849TrpSer: 0.849 ± 0.015	0.705TrpThr: 0.705 ± 0.011	0.73TrpVal: 0.73 ± 0.011	0.302TrpTrp: 0.302 ± 0.006	0.363TrpTyr: 0.363 ± 0.008	0.003TrpXaa: 0.003 ± 0.001
Tyr	1.419TyrAla: 1.419 ± 0.015	0.702TyrCys: 0.702 ± 0.01	1.435TyrAsp: 1.435 ± 0.014	1.631TyrGlu: 1.631 ± 0.017	1.32TyrPhe: 1.32 ± 0.015	1.655TyrGly: 1.655 ± 0.019	0.936TyrHis: 0.936 ± 0.012	1.427TyrIle: 1.427 ± 0.015	1.506TyrLys: 1.506 ± 0.02	2.754TyrLeu: 2.754 ± 0.025	0.711TyrMet: 0.711 ± 0.011	1.089TyrAsn: 1.089 ± 0.012	1.451TyrPro: 1.451 ± 0.014	1.21TyrGln: 1.21 ± 0.013	1.565TyrArg: 1.565 ± 0.015	2.192TyrSer: 2.192 ± 0.021	1.668TyrThr: 1.668 ± 0.018	1.676TyrVal: 1.676 ± 0.017	0.379TyrTrp: 0.379 ± 0.009	1.115TyrTyr: 1.115 ± 0.014	0.002TyrXaa: 0.002 ± 0.0
Xaa	0.016XaaAla: 0.016 ± 0.002	0.004XaaCys: 0.004 ± 0.001	0.004XaaAsp: 0.004 ± 0.001	0.006XaaGlu: 0.006 ± 0.001	0.004XaaPhe: 0.004 ± 0.001	0.016XaaGly: 0.016 ± 0.001	0.004XaaHis: 0.004 ± 0.001	0.002XaaIle: 0.002 ± 0.001	0.004XaaLys: 0.004 ± 0.001	0.012XaaLeu: 0.012 ± 0.001	0.003XaaMet: 0.003 ± 0.001	0.002XaaAsn: 0.002 ± 0.0	0.017XaaPro: 0.017 ± 0.001	0.005XaaGln: 0.005 ± 0.001	0.011XaaArg: 0.011 ± 0.001	0.011XaaSer: 0.011 ± 0.001	0.005XaaThr: 0.005 ± 0.001	0.007XaaVal: 0.007 ± 0.001	0.002XaaTrp: 0.002 ± 0.001	0.002XaaTyr: 0.002 ± 0.0	0.049XaaXaa: 0.049 ± 0.009

Statistics based on 16983 proteins (8071222 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---