Alteromonas phage vB_AcoS-R7M

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Amoyvirus; Alteromonas virus R7M

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3YNF1|A0A6M3YNF1_9CAUD DNA helicase/restriction enzyme OS=Alteromonas phage vB_AcoS-R7M OX=2729541 GN=vBAcoSR7M_53 PE=4 SV=1
MM1 pKa = 7.72AVTTLDD7 pKa = 3.54LVQTNGTPLPSEE19 pKa = 4.05LTVLEE24 pKa = 4.74GEE26 pKa = 4.37VEE28 pKa = 4.4YY29 pKa = 11.33NGSGLVAATAPAALNFNHH47 pKa = 7.52KK48 pKa = 9.09DD49 pKa = 3.4TAIVSYY55 pKa = 9.57TIVDD59 pKa = 3.71TALSDD64 pKa = 4.05DD65 pKa = 4.41LVVLQYY71 pKa = 10.86RR72 pKa = 11.84YY73 pKa = 8.72TNDD76 pKa = 3.04GSVTSVTIYY85 pKa = 11.0YY86 pKa = 10.13NFAISRR92 pKa = 11.84LTTVEE97 pKa = 4.29TIAGEE102 pKa = 4.09NIYY105 pKa = 10.81RR106 pKa = 11.84RR107 pKa = 11.84DD108 pKa = 3.69TFLSDD113 pKa = 3.46YY114 pKa = 11.2NFGDD118 pKa = 3.55MVKK121 pKa = 10.28ISVVPANDD129 pKa = 5.29LITIYY134 pKa = 10.66INGVRR139 pKa = 11.84VYY141 pKa = 10.81GYY143 pKa = 7.59EE144 pKa = 3.89TEE146 pKa = 4.23LNKK149 pKa = 10.62GVASSTIKK157 pKa = 10.99LNGTAPVLTEE167 pKa = 5.15LEE169 pKa = 4.34YY170 pKa = 10.91TDD172 pKa = 4.82SNEE175 pKa = 4.0GNSDD179 pKa = 3.14IQSPLAALPLSLIGKK194 pKa = 7.34STQFQKK200 pKa = 11.02LGIMHH205 pKa = 7.09NKK207 pKa = 8.42PSTMSFVRR215 pKa = 11.84EE216 pKa = 4.46FWAEE220 pKa = 4.05TVDD223 pKa = 3.38TSMFPGWPTTRR234 pKa = 11.84YY235 pKa = 7.97PRR237 pKa = 11.84ARR239 pKa = 11.84FSCTDD244 pKa = 3.19HH245 pKa = 7.09DD246 pKa = 4.3TGSGGIWVRR255 pKa = 11.84VYY257 pKa = 11.14DD258 pKa = 4.34KK259 pKa = 11.27DD260 pKa = 3.88LGAITDD266 pKa = 3.85PSSWLEE272 pKa = 3.65WDD274 pKa = 4.22DD275 pKa = 3.59VSSRR279 pKa = 11.84PEE281 pKa = 3.46FDD283 pKa = 3.83HH284 pKa = 7.6IEE286 pKa = 4.06RR287 pKa = 11.84KK288 pKa = 9.25INPVFVYY295 pKa = 10.41SGTGDD300 pKa = 3.36QTEE303 pKa = 4.53TPMHH307 pKa = 6.52LVAADD312 pKa = 4.07GDD314 pKa = 3.71ILMYY318 pKa = 9.21FHH320 pKa = 7.27NSTVTLEE327 pKa = 4.14GFPAGIQATHH337 pKa = 6.43YY338 pKa = 7.9ATTLNGIDD346 pKa = 3.88YY347 pKa = 8.5TDD349 pKa = 4.1PTPSSIVYY357 pKa = 10.23DD358 pKa = 3.68PTYY361 pKa = 11.0LEE363 pKa = 4.91GDD365 pKa = 3.35GHH367 pKa = 6.21TGYY370 pKa = 10.11FQPGRR375 pKa = 11.84NTFAEE380 pKa = 4.13IPYY383 pKa = 9.68KK384 pKa = 10.76YY385 pKa = 9.92IGIHH389 pKa = 4.66LHH391 pKa = 6.17GGAGASRR398 pKa = 11.84GSCWAIEE405 pKa = 4.05VSNDD409 pKa = 2.73GKK411 pKa = 10.56NWEE414 pKa = 3.96RR415 pKa = 11.84WRR417 pKa = 11.84TFMRR421 pKa = 11.84DD422 pKa = 3.33FQATKK427 pKa = 10.18RR428 pKa = 11.84LPYY431 pKa = 9.83TDD433 pKa = 5.03EE434 pKa = 3.76EE435 pKa = 4.81DD436 pKa = 3.73KK437 pKa = 11.28FFVLQRR443 pKa = 11.84VHH445 pKa = 5.03EE446 pKa = 4.36AKK448 pKa = 10.39RR449 pKa = 11.84VGGYY453 pKa = 9.21YY454 pKa = 9.69RR455 pKa = 11.84VWGRR459 pKa = 11.84WQRR462 pKa = 11.84EE463 pKa = 4.02AFGGRR468 pKa = 11.84LGEE471 pKa = 4.15LEE473 pKa = 4.13AVEE476 pKa = 4.4VLVDD480 pKa = 3.47SDD482 pKa = 4.05FNIVSEE488 pKa = 4.71CNVFIPRR495 pKa = 11.84GSEE498 pKa = 4.1GEE500 pKa = 3.67FDD502 pKa = 3.34AVEE505 pKa = 3.65IRR507 pKa = 11.84GYY509 pKa = 10.95FPFEE513 pKa = 4.03YY514 pKa = 10.6GGVEE518 pKa = 3.89YY519 pKa = 8.81ATYY522 pKa = 8.97STRR525 pKa = 11.84LGTTIDD531 pKa = 3.97DD532 pKa = 3.89EE533 pKa = 4.62SAIGIGIVTDD543 pKa = 3.87VNRR546 pKa = 11.84NWQIYY551 pKa = 10.38NGMANKK557 pKa = 10.04SRR559 pKa = 11.84TLLVDD564 pKa = 3.71RR565 pKa = 11.84SNFPEE570 pKa = 4.25KK571 pKa = 10.54VSSSEE576 pKa = 4.16TIVLKK581 pKa = 10.28TEE583 pKa = 3.98AGYY586 pKa = 10.42QYY588 pKa = 10.75QSLPIRR594 pKa = 11.84RR595 pKa = 11.84DD596 pKa = 3.21NSISTLISNDD606 pKa = 3.36PVDD609 pKa = 4.84LSGQITDD616 pKa = 3.45VTFKK620 pKa = 11.35NIGKK624 pKa = 10.14DD625 pKa = 3.21SAAPIYY631 pKa = 10.73LEE633 pKa = 4.83FGITDD638 pKa = 3.68SLSNPTQQLSISFDD652 pKa = 3.43TDD654 pKa = 2.96HH655 pKa = 7.55DD656 pKa = 4.58VVTGCIPMLMRR667 pKa = 11.84IIGTVGTQEE676 pKa = 3.7VRR678 pKa = 11.84SINVFGMDD686 pKa = 3.35NDD688 pKa = 3.8NPSVYY693 pKa = 8.82EE694 pKa = 3.91TANTKK699 pKa = 10.35HH700 pKa = 6.32EE701 pKa = 3.99LTIRR705 pKa = 11.84IDD707 pKa = 3.14KK708 pKa = 10.42HH709 pKa = 6.55NNEE712 pKa = 4.04IQFLSGGSVTDD723 pKa = 3.6TLPLAGFVADD733 pKa = 3.42TPMFVFIKK741 pKa = 10.49AGTIDD746 pKa = 3.79PDD748 pKa = 3.49EE749 pKa = 4.91TEE751 pKa = 4.53DD752 pKa = 3.98SSVSFNEE759 pKa = 3.88ISVQSATADD768 pKa = 3.47KK769 pKa = 10.9LVEE772 pKa = 4.41LVNAHH777 pKa = 7.34PIADD781 pKa = 4.35AGPDD785 pKa = 3.34QSGISAGSTFQLDD798 pKa = 3.53GSGSYY803 pKa = 10.91DD804 pKa = 3.15SDD806 pKa = 3.98GSIVGYY812 pKa = 10.19EE813 pKa = 3.87WVQVDD818 pKa = 3.44NGAPSVAPFDD828 pKa = 3.97TTAVQPMVTAPSIGTPTTLRR848 pKa = 11.84FEE850 pKa = 4.81LVVEE854 pKa = 4.77DD855 pKa = 4.71DD856 pKa = 4.91DD857 pKa = 5.45GAFSVADD864 pKa = 3.62AVNVEE869 pKa = 4.53VTSTPNVGPTANAGTPSTIEE889 pKa = 4.37AGSQYY894 pKa = 10.74QLNGSASDD902 pKa = 3.47SDD904 pKa = 3.77GTIVSTVWTQTGDD917 pKa = 3.29AVTLSNPNVLNPTFTAPLVSSGSTTFTLTVTDD949 pKa = 4.99DD950 pKa = 4.29DD951 pKa = 5.55GEE953 pKa = 4.56TATDD957 pKa = 3.61TVVITYY963 pKa = 9.65EE964 pKa = 4.02DD965 pKa = 3.34TTAPVITRR973 pKa = 11.84NGPATVTLNLNDD985 pKa = 3.86TFTPATFTAFDD996 pKa = 4.2AVDD999 pKa = 3.88GDD1001 pKa = 4.07LTSAITVVNPYY1012 pKa = 10.14QPATGEE1018 pKa = 4.15YY1019 pKa = 8.73TVTANVSDD1027 pKa = 3.98AAGNAATEE1035 pKa = 4.37VTQVVTVTDD1044 pKa = 4.74DD1045 pKa = 2.63ISYY1048 pKa = 9.8PFNTVSSEE1056 pKa = 4.03NIPFGNNVVLQTTIRR1071 pKa = 11.84LGSTRR1076 pKa = 11.84CFNVNLSSWCNPEE1089 pKa = 3.73EE1090 pKa = 4.54LYY1092 pKa = 11.01SHH1094 pKa = 6.61QIDD1097 pKa = 3.73VSGTGLSIVNSQIQGGILVFYY1118 pKa = 10.52AQGLAVGRR1126 pKa = 11.84HH1127 pKa = 4.98KK1128 pKa = 10.91FKK1130 pKa = 9.82ITYY1133 pKa = 7.49STQSRR1138 pKa = 11.84QDD1140 pKa = 3.01YY1141 pKa = 8.8FYY1143 pKa = 10.95AYY1145 pKa = 10.51VEE1147 pKa = 4.21VSSYY1151 pKa = 11.36EE1152 pKa = 4.03GLL1154 pKa = 3.64

Molecular weight: 125.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3YR60|A0A6M3YR60_9CAUD Uncharacterized protein OS=Alteromonas phage vB_AcoS-R7M OX=2729541 GN=vBAcoSR7M_2 PE=4 SV=1
MM1 pKa = 7.61SKK3 pKa = 8.66TAQQRR8 pKa = 11.84FDD10 pKa = 3.08EE11 pKa = 4.8TYY13 pKa = 7.62ITGYY17 pKa = 10.21EE18 pKa = 3.89IQTIMDD24 pKa = 3.82VNRR27 pKa = 11.84STVLNARR34 pKa = 11.84KK35 pKa = 9.68RR36 pKa = 11.84GILPQPIQVPGSRR49 pKa = 11.84SFIWEE54 pKa = 3.82RR55 pKa = 11.84TIVQPYY61 pKa = 7.9IDD63 pKa = 3.65AWKK66 pKa = 10.11VALASRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84EE75 pKa = 3.96LTGGVV80 pKa = 3.34

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

17771

31

1528

265.2

29.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.996 ± 0.498

1.109 ± 0.128

5.959 ± 0.187

7.231 ± 0.363

3.697 ± 0.14

7.282 ± 0.228

1.823 ± 0.159

5.638 ± 0.21

5.717 ± 0.398

8.047 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.124

4.997 ± 0.135

4.316 ± 0.211

3.962 ± 0.391

5.036 ± 0.199

6.387 ± 0.267

6.331 ± 0.413

6.702 ± 0.235

1.474 ± 0.138

3.826 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski