Oryzias latipes (Japanese rice fish) (Japanese killifish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B3IDI4|A0A3B3IDI4_ORYLA Uncharacterized protein OS=Oryzias latipes OX=8090 GN=ercc6l2 PE=4 SV=1
MM1 pKa = 7.25NLDD4 pKa = 3.51LKK6 pKa = 10.57VFRR9 pKa = 11.84TIKK12 pKa = 10.27SYY14 pKa = 11.28YY15 pKa = 6.48EE16 pKa = 3.97QKK18 pKa = 10.69GVPPAPRR25 pKa = 11.84PVPAPRR31 pKa = 11.84GVPPAPRR38 pKa = 11.84PVPAPRR44 pKa = 11.84GVPPAPRR51 pKa = 11.84RR52 pKa = 11.84VPSDD56 pKa = 3.46CWPDD60 pKa = 3.7PPEE63 pKa = 4.5PDD65 pKa = 3.7CWPDD69 pKa = 3.75PPEE72 pKa = 4.5PDD74 pKa = 3.7CWPDD78 pKa = 3.75PPEE81 pKa = 4.5PDD83 pKa = 3.7CWPDD87 pKa = 3.75PPEE90 pKa = 4.5PDD92 pKa = 3.7CWPDD96 pKa = 3.75PPEE99 pKa = 4.5PDD101 pKa = 3.7CWPDD105 pKa = 3.75PPEE108 pKa = 4.5PDD110 pKa = 3.7CWPDD114 pKa = 3.75PPEE117 pKa = 4.5PDD119 pKa = 3.7CWPDD123 pKa = 3.75PPEE126 pKa = 4.5PDD128 pKa = 3.7CWPDD132 pKa = 3.75PPEE135 pKa = 4.5PDD137 pKa = 3.7CWPDD141 pKa = 3.75PPEE144 pKa = 4.5PDD146 pKa = 3.7CWPDD150 pKa = 3.33PPEE153 pKa = 4.48RR154 pKa = 11.84CSGQIKK160 pKa = 10.12PILNFLVTMM169 pKa = 5.23

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B3HLF6|A0A3B3HLF6_ORYLA DZF domain-containing protein OS=Oryzias latipes OX=8090 GN=zfr PE=4 SV=1
RR1 pKa = 7.11AVRR4 pKa = 11.84RR5 pKa = 11.84GRR7 pKa = 11.84SGGRR11 pKa = 11.84LGGVGHH17 pKa = 6.24GVRR20 pKa = 11.84LGGVGHH26 pKa = 6.11GVRR29 pKa = 11.84LGGVGHH35 pKa = 6.11GVRR38 pKa = 11.84LGGVGHH44 pKa = 6.54GARR47 pKa = 11.84LGGVGHH53 pKa = 6.65GARR56 pKa = 11.84LGGVGHH62 pKa = 6.65GARR65 pKa = 11.84LGGVGHH71 pKa = 6.21GVRR74 pKa = 11.84LGGVGPAVRR83 pKa = 11.84LWRR86 pKa = 11.84VGRR89 pKa = 3.84

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23617

12521

36138

21724183

15

9615

601.1

67.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.543 ± 0.012

2.249 ± 0.011

5.095 ± 0.009

6.816 ± 0.021

3.721 ± 0.01

6.225 ± 0.018

2.71 ± 0.012

4.376 ± 0.012

5.701 ± 0.015

9.546 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.005

3.821 ± 0.007

5.874 ± 0.016

4.726 ± 0.011

5.715 ± 0.012

8.974 ± 0.018

5.557 ± 0.016

6.265 ± 0.013

1.152 ± 0.005

2.625 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski