Firmicutes bacterium CAG:791

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5C9X6|R5C9X6_9FIRM DNA replication and repair protein RecF OS=Firmicutes bacterium CAG:791 OX=1262993 GN=recF PE=3 SV=1
MM1 pKa = 7.81KK2 pKa = 10.32KK3 pKa = 10.3RR4 pKa = 11.84IVSVLMAGALASAMLAGCGNSSAPAASAAAEE35 pKa = 4.18DD36 pKa = 4.01TAEE39 pKa = 4.36DD40 pKa = 3.74TTEE43 pKa = 4.0EE44 pKa = 3.97ADD46 pKa = 3.45AAPAEE51 pKa = 4.21LDD53 pKa = 3.27YY54 pKa = 11.77DD55 pKa = 4.03GDD57 pKa = 3.83IKK59 pKa = 11.41VWVADD64 pKa = 3.61NAVSFTQEE72 pKa = 3.6QIAKK76 pKa = 9.18FQEE79 pKa = 4.48ANPGVANATFTVEE92 pKa = 4.37AVGEE96 pKa = 4.0GDD98 pKa = 3.24AASNVITDD106 pKa = 3.45VKK108 pKa = 11.02AGGDD112 pKa = 3.62VYY114 pKa = 11.09TFVQDD119 pKa = 3.23QIARR123 pKa = 11.84FVAAGALEE131 pKa = 4.24EE132 pKa = 4.17VAPEE136 pKa = 3.64NVAAVEE142 pKa = 3.97ADD144 pKa = 3.39NDD146 pKa = 3.7EE147 pKa = 4.6GAVGAATVGSTLYY160 pKa = 10.47AYY162 pKa = 9.37PLTSDD167 pKa = 3.07NGYY170 pKa = 10.88FMYY173 pKa = 10.53YY174 pKa = 10.07DD175 pKa = 3.62KK176 pKa = 11.45SVVTDD181 pKa = 3.76PTDD184 pKa = 3.64LDD186 pKa = 5.22AIIADD191 pKa = 4.07CEE193 pKa = 4.16KK194 pKa = 10.61AGKK197 pKa = 9.08NIYY200 pKa = 9.33MEE202 pKa = 5.05INSGWYY208 pKa = 6.59QTAFFFGTGCDD219 pKa = 3.53LSYY222 pKa = 10.38EE223 pKa = 4.13TDD225 pKa = 3.41DD226 pKa = 4.57AGNFTSANVNYY237 pKa = 10.49ASDD240 pKa = 3.5QGLVALKK247 pKa = 10.63EE248 pKa = 4.21MIRR251 pKa = 11.84LSSSSAFVNGSAAGEE266 pKa = 4.08ATTIGAIVDD275 pKa = 4.22GTWDD279 pKa = 3.27ATIVKK284 pKa = 9.7EE285 pKa = 4.7VFGDD289 pKa = 3.74NYY291 pKa = 10.98GCTPLPSFQGSDD303 pKa = 2.67GKK305 pKa = 9.25TYY307 pKa = 10.88QMSGFSGYY315 pKa = 10.68KK316 pKa = 9.92LLGVKK321 pKa = 9.45PQEE324 pKa = 4.73DD325 pKa = 4.09EE326 pKa = 4.24NKK328 pKa = 10.59LAVCDD333 pKa = 4.12ALAAYY338 pKa = 9.69LSSGEE343 pKa = 4.17VQLARR348 pKa = 11.84YY349 pKa = 7.46EE350 pKa = 4.34AIGWGPSNLEE360 pKa = 3.81AQQSDD365 pKa = 3.96EE366 pKa = 4.27VQADD370 pKa = 3.76EE371 pKa = 5.53ALSALNEE378 pKa = 3.78QLLRR382 pKa = 11.84NKK384 pKa = 10.01PQGQYY389 pKa = 10.31PGDD392 pKa = 3.74YY393 pKa = 7.35WTLATGLGDD402 pKa = 5.46DD403 pKa = 5.28IITGTLNEE411 pKa = 4.37KK412 pKa = 10.74SSDD415 pKa = 3.9EE416 pKa = 4.28DD417 pKa = 4.55LMAALQTFQDD427 pKa = 3.63TCISYY432 pKa = 11.24AKK434 pKa = 10.53

Molecular weight: 45.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5CA28|R5CA28_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:791 OX=1262993 GN=BN785_00896 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 8.07MTYY6 pKa = 8.55QPKK9 pKa = 9.46KK10 pKa = 7.57RR11 pKa = 11.84QRR13 pKa = 11.84AKK15 pKa = 9.44VHH17 pKa = 5.53GFRR20 pKa = 11.84ARR22 pKa = 11.84MASAGGRR29 pKa = 11.84KK30 pKa = 8.85VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.11GRR40 pKa = 11.84KK41 pKa = 8.21QLTVV45 pKa = 2.97

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2175

0

2175

699191

29

1537

321.5

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.438 ± 0.061

1.55 ± 0.022

5.759 ± 0.05

7.417 ± 0.065

4.076 ± 0.036

7.489 ± 0.046

2.007 ± 0.025

6.468 ± 0.051

5.518 ± 0.041

9.455 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.021 ± 0.026

3.786 ± 0.03

3.692 ± 0.03

3.269 ± 0.027

5.497 ± 0.051

5.869 ± 0.039

5.178 ± 0.038

6.733 ± 0.044

0.955 ± 0.017

3.821 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski