Amino acid dipepetide frequency for Firmicutes bacterium CAG:791

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.355AlaAla: 10.355 ± 0.168	1.26AlaCys: 1.26 ± 0.045	5.356AlaAsp: 5.356 ± 0.103	6.752AlaGlu: 6.752 ± 0.122	3.212AlaPhe: 3.212 ± 0.066	7.627AlaGly: 7.627 ± 0.113	1.256AlaHis: 1.256 ± 0.043	4.771AlaIle: 4.771 ± 0.097	4.268AlaLys: 4.268 ± 0.084	7.79AlaLeu: 7.79 ± 0.128	2.614AlaMet: 2.614 ± 0.066	2.413AlaAsn: 2.413 ± 0.06	2.567AlaPro: 2.567 ± 0.071	2.612AlaGln: 2.612 ± 0.067	3.786AlaArg: 3.786 ± 0.079	4.678AlaSer: 4.678 ± 0.087	2.85AlaThr: 2.85 ± 0.08	6.855AlaVal: 6.855 ± 0.115	0.699AlaTrp: 0.699 ± 0.035	2.656AlaTyr: 2.656 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	1.181CysAla: 1.181 ± 0.045	0.35CysCys: 0.35 ± 0.024	0.867CysAsp: 0.867 ± 0.04	0.891CysGlu: 0.891 ± 0.033	0.635CysPhe: 0.635 ± 0.035	1.595CysGly: 1.595 ± 0.046	0.335CysHis: 0.335 ± 0.024	1.034CysIle: 1.034 ± 0.043	0.576CysLys: 0.576 ± 0.033	1.373CysLeu: 1.373 ± 0.051	0.479CysMet: 0.479 ± 0.028	0.515CysAsn: 0.515 ± 0.029	0.668CysPro: 0.668 ± 0.035	0.328CysGln: 0.328 ± 0.026	1.12CysArg: 1.12 ± 0.045	1.001CysSer: 1.001 ± 0.042	0.755CysThr: 0.755 ± 0.036	1.066CysVal: 1.066 ± 0.038	0.154CysTrp: 0.154 ± 0.015	0.578CysTyr: 0.578 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	5.024AspAla: 5.024 ± 0.089	0.764AspCys: 0.764 ± 0.034	2.951AspAsp: 2.951 ± 0.08	4.312AspGlu: 4.312 ± 0.097	2.596AspPhe: 2.596 ± 0.064	4.827AspGly: 4.827 ± 0.093	1.226AspHis: 1.226 ± 0.045	3.623AspIle: 3.623 ± 0.086	2.481AspLys: 2.481 ± 0.062	5.348AspLeu: 5.348 ± 0.093	1.556AspMet: 1.556 ± 0.047	1.836AspAsn: 1.836 ± 0.05	2.636AspPro: 2.636 ± 0.069	1.681AspGln: 1.681 ± 0.045	3.372AspArg: 3.372 ± 0.074	3.122AspSer: 3.122 ± 0.078	3.011AspThr: 3.011 ± 0.106	3.863AspVal: 3.863 ± 0.081	0.596AspTrp: 0.596 ± 0.028	2.765AspTyr: 2.765 ± 0.074	0.0AspXaa: 0.0 ± 0.0
Glu	6.254GluAla: 6.254 ± 0.102	0.708GluCys: 0.708 ± 0.032	4.477GluAsp: 4.477 ± 0.085	7.596GluGlu: 7.596 ± 0.158	2.384GluPhe: 2.384 ± 0.055	4.565GluGly: 4.565 ± 0.088	1.522GluHis: 1.522 ± 0.053	4.96GluIle: 4.96 ± 0.091	6.173GluLys: 6.173 ± 0.128	6.659GluLeu: 6.659 ± 0.111	2.291GluMet: 2.291 ± 0.054	3.844GluAsn: 3.844 ± 0.075	1.997GluPro: 1.997 ± 0.058	2.826GluGln: 2.826 ± 0.07	3.641GluArg: 3.641 ± 0.094	3.311GluSer: 3.311 ± 0.078	3.733GluThr: 3.733 ± 0.079	3.99GluVal: 3.99 ± 0.081	0.601GluTrp: 0.601 ± 0.032	2.637GluTyr: 2.637 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.374PheAla: 3.374 ± 0.07	0.841PheCys: 0.841 ± 0.033	2.437PheAsp: 2.437 ± 0.062	2.409PheGlu: 2.409 ± 0.05	1.942PhePhe: 1.942 ± 0.068	3.245PheGly: 3.245 ± 0.074	1.018PheHis: 1.018 ± 0.043	2.18PheIle: 2.18 ± 0.064	1.296PheLys: 1.296 ± 0.046	4.514PheLeu: 4.514 ± 0.096	1.044PheMet: 1.044 ± 0.041	1.207PheAsn: 1.207 ± 0.051	1.635PhePro: 1.635 ± 0.049	1.237PheGln: 1.237 ± 0.04	2.582PheArg: 2.582 ± 0.067	2.767PheSer: 2.767 ± 0.062	2.18PheThr: 2.18 ± 0.063	2.706PheVal: 2.706 ± 0.076	0.489PheTrp: 0.489 ± 0.028	1.659PheTyr: 1.659 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	5.788GlyAla: 5.788 ± 0.12	1.363GlyCys: 1.363 ± 0.043	3.893GlyAsp: 3.893 ± 0.081	5.023GlyGlu: 5.023 ± 0.098	3.167GlyPhe: 3.167 ± 0.071	5.25GlyGly: 5.25 ± 0.11	1.433GlyHis: 1.433 ± 0.049	6.026GlyIle: 6.026 ± 0.109	5.369GlyLys: 5.369 ± 0.086	6.07GlyLeu: 6.07 ± 0.102	2.71GlyMet: 2.71 ± 0.06	3.026GlyAsn: 3.026 ± 0.071	1.688GlyPro: 1.688 ± 0.055	2.142GlyGln: 2.142 ± 0.056	4.065GlyArg: 4.065 ± 0.081	4.499GlySer: 4.499 ± 0.087	4.57GlyThr: 4.57 ± 0.097	5.046GlyVal: 5.046 ± 0.097	0.717GlyTrp: 0.717 ± 0.036	3.042GlyTyr: 3.042 ± 0.062	0.001GlyXaa: 0.001 ± 0.001
His	1.512HisAla: 1.512 ± 0.042	0.393HisCys: 0.393 ± 0.026	1.074HisAsp: 1.074 ± 0.042	1.171HisGlu: 1.171 ± 0.04	0.997HisPhe: 0.997 ± 0.039	1.559HisGly: 1.559 ± 0.05	0.551HisHis: 0.551 ± 0.036	1.303HisIle: 1.303 ± 0.045	0.847HisLys: 0.847 ± 0.035	1.935HisLeu: 1.935 ± 0.057	0.518HisMet: 0.518 ± 0.032	0.689HisAsn: 0.689 ± 0.036	1.103HisPro: 1.103 ± 0.04	0.699HisGln: 0.699 ± 0.033	1.18HisArg: 1.18 ± 0.044	1.18HisSer: 1.18 ± 0.035	0.994HisThr: 0.994 ± 0.034	1.291HisVal: 1.291 ± 0.045	0.213HisTrp: 0.213 ± 0.019	0.862HisTyr: 0.862 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	5.648IleAla: 5.648 ± 0.098	1.183IleCys: 1.183 ± 0.047	3.415IleAsp: 3.415 ± 0.069	3.463IleGlu: 3.463 ± 0.078	2.534IlePhe: 2.534 ± 0.069	4.986IleGly: 4.986 ± 0.09	1.562IleHis: 1.562 ± 0.048	3.534IleIle: 3.534 ± 0.084	2.207IleLys: 2.207 ± 0.064	7.072IleLeu: 7.072 ± 0.115	1.622IleMet: 1.622 ± 0.053	2.177IleAsn: 2.177 ± 0.064	3.268IlePro: 3.268 ± 0.064	2.017IleGln: 2.017 ± 0.056	4.693IleArg: 4.693 ± 0.083	4.382IleSer: 4.382 ± 0.099	3.564IleThr: 3.564 ± 0.072	4.398IleVal: 4.398 ± 0.085	0.589IleTrp: 0.589 ± 0.03	2.371IleTyr: 2.371 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	4.87LysAla: 4.87 ± 0.097	0.628LysCys: 0.628 ± 0.033	3.395LysAsp: 3.395 ± 0.074	5.346LysGlu: 5.346 ± 0.103	1.47LysPhe: 1.47 ± 0.045	3.631LysGly: 3.631 ± 0.067	0.828LysHis: 0.828 ± 0.037	3.423LysIle: 3.423 ± 0.064	4.437LysLys: 4.437 ± 0.092	4.771LysLeu: 4.771 ± 0.089	1.6LysMet: 1.6 ± 0.05	2.559LysAsn: 2.559 ± 0.053	1.888LysPro: 1.888 ± 0.051	1.708LysGln: 1.708 ± 0.053	2.833LysArg: 2.833 ± 0.065	2.562LysSer: 2.562 ± 0.056	3.227LysThr: 3.227 ± 0.076	3.461LysVal: 3.461 ± 0.075	0.475LysTrp: 0.475 ± 0.027	2.064LysTyr: 2.064 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	7.699LeuAla: 7.699 ± 0.141	1.731LeuCys: 1.731 ± 0.053	4.926LeuAsp: 4.926 ± 0.091	6.058LeuGlu: 6.058 ± 0.102	4.245LeuPhe: 4.245 ± 0.111	6.13LeuGly: 6.13 ± 0.102	2.1LeuHis: 2.1 ± 0.063	5.854LeuIle: 5.854 ± 0.108	4.69LeuLys: 4.69 ± 0.076	10.102LeuLeu: 10.102 ± 0.179	2.629LeuMet: 2.629 ± 0.063	3.377LeuAsn: 3.377 ± 0.072	4.346LeuPro: 4.346 ± 0.088	3.593LeuGln: 3.593 ± 0.081	5.195LeuArg: 5.195 ± 0.11	6.59LeuSer: 6.59 ± 0.12	5.266LeuThr: 5.266 ± 0.093	5.814LeuVal: 5.814 ± 0.099	0.844LeuTrp: 0.844 ± 0.034	3.368LeuTyr: 3.368 ± 0.091	0.0LeuXaa: 0.0 ± 0.0
Met	2.534MetAla: 2.534 ± 0.065	0.366MetCys: 0.366 ± 0.024	1.832MetAsp: 1.832 ± 0.051	2.318MetGlu: 2.318 ± 0.06	0.97MetPhe: 0.97 ± 0.04	1.965MetGly: 1.965 ± 0.057	0.598MetHis: 0.598 ± 0.029	2.078MetIle: 2.078 ± 0.055	2.131MetLys: 2.131 ± 0.051	2.577MetLeu: 2.577 ± 0.067	0.96MetMet: 0.96 ± 0.042	1.469MetAsn: 1.469 ± 0.039	1.144MetPro: 1.144 ± 0.039	1.201MetGln: 1.201 ± 0.038	1.463MetArg: 1.463 ± 0.051	1.766MetSer: 1.766 ± 0.052	1.938MetThr: 1.938 ± 0.048	1.842MetVal: 1.842 ± 0.053	0.243MetTrp: 0.243 ± 0.018	0.808MetTyr: 0.808 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.248AsnAla: 3.248 ± 0.067	0.546AsnCys: 0.546 ± 0.027	1.962AsnAsp: 1.962 ± 0.052	2.413AsnGlu: 2.413 ± 0.06	1.44AsnPhe: 1.44 ± 0.055	3.43AsnGly: 3.43 ± 0.079	0.862AsnHis: 0.862 ± 0.034	2.5AsnIle: 2.5 ± 0.062	1.676AsnLys: 1.676 ± 0.057	3.547AsnLeu: 3.547 ± 0.074	1.12AsnMet: 1.12 ± 0.042	1.273AsnAsn: 1.273 ± 0.056	1.951AsnPro: 1.951 ± 0.043	1.19AsnGln: 1.19 ± 0.044	2.163AsnArg: 2.163 ± 0.052	2.082AsnSer: 2.082 ± 0.056	1.879AsnThr: 1.879 ± 0.044	2.629AsnVal: 2.629 ± 0.069	0.409AsnTrp: 0.409 ± 0.029	1.539AsnTyr: 1.539 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	3.265ProAla: 3.265 ± 0.076	0.548ProCys: 0.548 ± 0.027	2.832ProAsp: 2.832 ± 0.079	4.088ProGlu: 4.088 ± 0.086	1.698ProPhe: 1.698 ± 0.051	2.818ProGly: 2.818 ± 0.067	0.718ProHis: 0.718 ± 0.033	1.872ProIle: 1.872 ± 0.058	1.822ProLys: 1.822 ± 0.054	3.028ProLeu: 3.028 ± 0.082	1.003ProMet: 1.003 ± 0.041	1.16ProAsn: 1.16 ± 0.045	0.749ProPro: 0.749 ± 0.034	1.187ProGln: 1.187 ± 0.043	1.23ProArg: 1.23 ± 0.047	2.16ProSer: 2.16 ± 0.057	1.425ProThr: 1.425 ± 0.049	3.361ProVal: 3.361 ± 0.079	0.403ProTrp: 0.403 ± 0.025	1.552ProTyr: 1.552 ± 0.048	0.004ProXaa: 0.004 ± 0.002
Gln	2.567GlnAla: 2.567 ± 0.076	0.358GlnCys: 0.358 ± 0.022	1.763GlnAsp: 1.763 ± 0.051	2.723GlnGlu: 2.723 ± 0.061	1.074GlnPhe: 1.074 ± 0.043	1.984GlnGly: 1.984 ± 0.054	0.596GlnHis: 0.596 ± 0.029	2.413GlnIle: 2.413 ± 0.062	2.623GlnLys: 2.623 ± 0.063	2.89GlnLeu: 2.89 ± 0.075	1.271GlnMet: 1.271 ± 0.039	1.713GlnAsn: 1.713 ± 0.052	1.005GlnPro: 1.005 ± 0.037	1.239GlnGln: 1.239 ± 0.053	1.64GlnArg: 1.64 ± 0.056	1.66GlnSer: 1.66 ± 0.051	1.689GlnThr: 1.689 ± 0.054	1.944GlnVal: 1.944 ± 0.055	0.262GlnTrp: 0.262 ± 0.018	1.273GlnTyr: 1.273 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	3.638ArgAla: 3.638 ± 0.083	0.811ArgCys: 0.811 ± 0.038	2.879ArgAsp: 2.879 ± 0.067	4.664ArgGlu: 4.664 ± 0.109	2.213ArgPhe: 2.213 ± 0.055	3.028ArgGly: 3.028 ± 0.069	1.104ArgHis: 1.104 ± 0.044	4.447ArgIle: 4.447 ± 0.083	4.159ArgLys: 4.159 ± 0.088	4.78ArgLeu: 4.78 ± 0.089	1.951ArgMet: 1.951 ± 0.056	2.42ArgAsn: 2.42 ± 0.057	1.721ArgPro: 1.721 ± 0.057	1.942ArgGln: 1.942 ± 0.067	3.614ArgArg: 3.614 ± 0.1	3.011ArgSer: 3.011 ± 0.07	2.835ArgThr: 2.835 ± 0.067	3.138ArgVal: 3.138 ± 0.07	0.481ArgTrp: 0.481 ± 0.033	2.134ArgTyr: 2.134 ± 0.062	0.0ArgXaa: 0.0 ± 0.0
Ser	4.781SerAla: 4.781 ± 0.087	0.978SerCys: 0.978 ± 0.038	3.39SerAsp: 3.39 ± 0.075	4.088SerGlu: 4.088 ± 0.082	2.926SerPhe: 2.926 ± 0.07	5.638SerGly: 5.638 ± 0.114	1.023SerHis: 1.023 ± 0.039	3.587SerIle: 3.587 ± 0.081	2.446SerLys: 2.446 ± 0.059	5.438SerLeu: 5.438 ± 0.099	1.884SerMet: 1.884 ± 0.056	1.891SerAsn: 1.891 ± 0.055	1.781SerPro: 1.781 ± 0.052	1.676SerGln: 1.676 ± 0.051	3.344SerArg: 3.344 ± 0.069	3.84SerSer: 3.84 ± 0.09	2.639SerThr: 2.639 ± 0.066	4.298SerVal: 4.298 ± 0.093	0.668SerTrp: 0.668 ± 0.033	2.38SerTyr: 2.38 ± 0.064	0.001SerXaa: 0.001 ± 0.001
Thr	4.664ThrAla: 4.664 ± 0.089	0.718ThrCys: 0.718 ± 0.028	3.473ThrAsp: 3.473 ± 0.096	3.77ThrGlu: 3.77 ± 0.097	2.068ThrPhe: 2.068 ± 0.06	4.846ThrGly: 4.846 ± 0.102	0.891ThrHis: 0.891 ± 0.034	3.497ThrIle: 3.497 ± 0.073	2.429ThrLys: 2.429 ± 0.062	4.565ThrLeu: 4.565 ± 0.079	1.452ThrMet: 1.452 ± 0.045	1.686ThrAsn: 1.686 ± 0.052	2.102ThrPro: 2.102 ± 0.06	1.502ThrGln: 1.502 ± 0.053	2.336ThrArg: 2.336 ± 0.065	2.737ThrSer: 2.737 ± 0.066	2.6ThrThr: 2.6 ± 0.062	4.126ThrVal: 4.126 ± 0.083	0.496ThrTrp: 0.496 ± 0.03	1.822ThrTyr: 1.822 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	4.467ValAla: 4.467 ± 0.088	1.261ValCys: 1.261 ± 0.039	3.597ValAsp: 3.597 ± 0.07	3.757ValGlu: 3.757 ± 0.085	3.058ValPhe: 3.058 ± 0.082	4.158ValGly: 4.158 ± 0.094	1.314ValHis: 1.314 ± 0.046	4.86ValIle: 4.86 ± 0.1	3.364ValLys: 3.364 ± 0.068	7.233ValLeu: 7.233 ± 0.127	2.227ValMet: 2.227 ± 0.062	2.583ValAsn: 2.583 ± 0.069	2.899ValPro: 2.899 ± 0.067	2.167ValGln: 2.167 ± 0.054	3.8ValArg: 3.8 ± 0.087	4.59ValSer: 4.59 ± 0.087	4.155ValThr: 4.155 ± 0.078	4.557ValVal: 4.557 ± 0.099	0.705ValTrp: 0.705 ± 0.034	2.58ValTyr: 2.58 ± 0.061	0.0ValXaa: 0.0 ± 0.0
Trp	0.589TrpAla: 0.589 ± 0.032	0.137TrpCys: 0.137 ± 0.014	0.532TrpAsp: 0.532 ± 0.027	0.626TrpGlu: 0.626 ± 0.031	0.455TrpPhe: 0.455 ± 0.025	0.631TrpGly: 0.631 ± 0.03	0.212TrpHis: 0.212 ± 0.019	0.644TrpIle: 0.644 ± 0.031	0.757TrpLys: 0.757 ± 0.035	0.938TrpLeu: 0.938 ± 0.041	0.326TrpMet: 0.326 ± 0.02	0.568TrpAsn: 0.568 ± 0.033	0.273TrpPro: 0.273 ± 0.018	0.366TrpGln: 0.366 ± 0.025	0.461TrpArg: 0.461 ± 0.026	0.545TrpSer: 0.545 ± 0.031	0.458TrpThr: 0.458 ± 0.029	0.492TrpVal: 0.492 ± 0.025	0.112TrpTrp: 0.112 ± 0.013	0.425TrpTyr: 0.425 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.919TyrAla: 2.919 ± 0.073	0.556TyrCys: 0.556 ± 0.026	2.539TyrAsp: 2.539 ± 0.061	2.702TyrGlu: 2.702 ± 0.072	1.678TyrPhe: 1.678 ± 0.045	2.976TyrGly: 2.976 ± 0.066	0.924TyrHis: 0.924 ± 0.038	2.081TyrIle: 2.081 ± 0.059	1.426TyrLys: 1.426 ± 0.049	3.925TyrLeu: 3.925 ± 0.084	0.948TyrMet: 0.948 ± 0.037	1.453TyrAsn: 1.453 ± 0.052	1.497TyrPro: 1.497 ± 0.054	1.377TyrGln: 1.377 ± 0.052	2.45TyrArg: 2.45 ± 0.075	2.21TyrSer: 2.21 ± 0.059	2.012TyrThr: 2.012 ± 0.056	2.454TyrVal: 2.454 ± 0.058	0.388TyrTrp: 0.388 ± 0.026	1.698TyrTyr: 1.698 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.005

Statistics based on 2175 proteins (699191 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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