candidate division MSBL1 archaeon SCGC-AAA833K04
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133VSK1|A0A133VSK1_9EURY Translation initiation factor 5A OS=candidate division MSBL1 archaeon SCGC-AAA833K04 OX=1698258 GN=eif5a PE=3 SV=1
MM1 pKa = 7.4 EE2 pKa = 5.42 LWQVVYY8 pKa = 10.52 IIGVFAGLAVAGTISPYY25 pKa = 10.22 VAVAFIWAFIIISEE39 pKa = 4.3 VTHH42 pKa = 5.6 YY43 pKa = 10.11 WGEE46 pKa = 3.97 LKK48 pKa = 10.52 KK49 pKa = 10.89 FRR51 pKa = 11.84 VEE53 pKa = 3.74 VEE55 pKa = 3.92 EE56 pKa = 4.56 AGEE59 pKa = 3.91 EE60 pKa = 4.19 LEE62 pKa = 4.53 EE63 pKa = 4.96 RR64 pKa = 11.84 GEE66 pKa = 4.23 PIEE69 pKa = 5.05 AGEE72 pKa = 4.07 EE73 pKa = 4.18 TIGEE77 pKa = 4.33 SEE79 pKa = 4.22 EE80 pKa = 4.39 GG81 pKa = 3.43
Molecular weight: 9.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.275
IPC2_protein 4.228
IPC_protein 4.037
Toseland 3.923
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.745
Rodwell 3.884
Grimsley 3.846
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.037
Thurlkill 3.91
EMBOSS 3.77
Sillero 4.126
Patrickios 3.3
IPC_peptide 3.935
IPC2_peptide 4.126
IPC2.peptide.svr19 4.056
Protein with the highest isoelectric point:
>tr|A0A133VSG9|A0A133VSG9_9EURY 50S ribosomal protein L15 OS=candidate division MSBL1 archaeon SCGC-AAA833K04 OX=1698258 GN=rpl15 PE=3 SV=1
MM1 pKa = 7.3 TNKK4 pKa = 9.63 KK5 pKa = 9.67 RR6 pKa = 11.84 DD7 pKa = 3.55 KK8 pKa = 10.99 KK9 pKa = 10.7 KK10 pKa = 10.54 GANICSRR17 pKa = 11.84 CGSRR21 pKa = 11.84 GAVFQRR27 pKa = 11.84 LGLNLCRR34 pKa = 11.84 RR35 pKa = 11.84 CFRR38 pKa = 11.84 EE39 pKa = 4.06 VASEE43 pKa = 4.05 MGFKK47 pKa = 10.45 KK48 pKa = 10.67 YY49 pKa = 10.09 RR50 pKa = 3.41
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.706
IPC_protein 10.292
Toseland 10.965
ProMoST 11.008
Dawson 11.023
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.33
Grimsley 11.038
Solomon 11.169
Lehninger 11.14
Nozaki 10.965
DTASelect 10.687
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.95
Patrickios 11.096
IPC_peptide 11.169
IPC2_peptide 9.955
IPC2.peptide.svr19 8.783
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
196
0
196
41341
50
863
210.9
23.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.056 ± 0.187
0.866 ± 0.07
5.261 ± 0.157
9.547 ± 0.269
3.432 ± 0.128
7.591 ± 0.166
1.691 ± 0.078
7.138 ± 0.168
7.68 ± 0.222
9.535 ± 0.223
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.082
3.251 ± 0.101
4.032 ± 0.118
2.595 ± 0.098
6.156 ± 0.204
6.086 ± 0.135
4.874 ± 0.108
7.254 ± 0.173
1.113 ± 0.064
2.588 ± 0.092
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here