candidate division MSBL1 archaeon SCGC-AAA833K04

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A133VSK1|A0A133VSK1_9EURY Translation initiation factor 5A OS=candidate division MSBL1 archaeon SCGC-AAA833K04 OX=1698258 GN=eif5a PE=3 SV=1
MM1 pKa = 7.4EE2 pKa = 5.42LWQVVYY8 pKa = 10.52IIGVFAGLAVAGTISPYY25 pKa = 10.22VAVAFIWAFIIISEE39 pKa = 4.3VTHH42 pKa = 5.6YY43 pKa = 10.11WGEE46 pKa = 3.97LKK48 pKa = 10.52KK49 pKa = 10.89FRR51 pKa = 11.84VEE53 pKa = 3.74VEE55 pKa = 3.92EE56 pKa = 4.56AGEE59 pKa = 3.91EE60 pKa = 4.19LEE62 pKa = 4.53EE63 pKa = 4.96RR64 pKa = 11.84GEE66 pKa = 4.23PIEE69 pKa = 5.05AGEE72 pKa = 4.07EE73 pKa = 4.18TIGEE77 pKa = 4.33SEE79 pKa = 4.22EE80 pKa = 4.39GG81 pKa = 3.43

Molecular weight:
9.02 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A133VSG9|A0A133VSG9_9EURY 50S ribosomal protein L15 OS=candidate division MSBL1 archaeon SCGC-AAA833K04 OX=1698258 GN=rpl15 PE=3 SV=1
MM1 pKa = 7.3TNKK4 pKa = 9.63KK5 pKa = 9.67RR6 pKa = 11.84DD7 pKa = 3.55KK8 pKa = 10.99KK9 pKa = 10.7KK10 pKa = 10.54GANICSRR17 pKa = 11.84CGSRR21 pKa = 11.84GAVFQRR27 pKa = 11.84LGLNLCRR34 pKa = 11.84RR35 pKa = 11.84CFRR38 pKa = 11.84EE39 pKa = 4.06VASEE43 pKa = 4.05MGFKK47 pKa = 10.45KK48 pKa = 10.67YY49 pKa = 10.09RR50 pKa = 3.41

Molecular weight:
5.8 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

196

0

196

41341

50

863

210.9

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.056 ± 0.187

0.866 ± 0.07

5.261 ± 0.157

9.547 ± 0.269

3.432 ± 0.128

7.591 ± 0.166

1.691 ± 0.078

7.138 ± 0.168

7.68 ± 0.222

9.535 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.082

3.251 ± 0.101

4.032 ± 0.118

2.595 ± 0.098

6.156 ± 0.204

6.086 ± 0.135

4.874 ± 0.108

7.254 ± 0.173

1.113 ± 0.064

2.588 ± 0.092

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski