Ascoidea rubescens DSM 1968

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Ascoideaceae; Ascoidea; Ascoidea rubescens

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D2VMV0|A0A1D2VMV0_9ASCO O-acetylhomoserine ami OS=Ascoidea rubescens DSM 1968 OX=1344418 GN=ASCRUDRAFT_68727 PE=3 SV=1
MM1 pKa = 7.36VYY3 pKa = 9.19STFTSDD9 pKa = 3.22NADD12 pKa = 4.32FININDD18 pKa = 3.82AVQITHH24 pKa = 7.7LLHH27 pKa = 6.53NHH29 pKa = 7.0DD30 pKa = 5.13EE31 pKa = 5.01PIQSKK36 pKa = 8.6DD37 pKa = 3.44TNPSIVEE44 pKa = 3.69VDD46 pKa = 3.49AVEE49 pKa = 5.1LYY51 pKa = 11.01NNNLGRR57 pKa = 11.84TRR59 pKa = 11.84TSSFSSEE66 pKa = 3.67INSPSEE72 pKa = 3.87EE73 pKa = 4.19VVDD76 pKa = 4.18PFTNTEE82 pKa = 3.91NKK84 pKa = 9.21VAEE87 pKa = 4.15VDD89 pKa = 3.86YY90 pKa = 8.6FTSDD94 pKa = 3.15EE95 pKa = 4.57LVWQPVVSSFADD107 pKa = 3.5DD108 pKa = 3.93FNSVGDD114 pKa = 3.55VDD116 pKa = 4.3YY117 pKa = 11.32YY118 pKa = 11.38QYY120 pKa = 12.0

Molecular weight: 13.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D2VQS6|A0A1D2VQS6_9ASCO Exosome complex exonuclease RRP44 OS=Ascoidea rubescens DSM 1968 OX=1344418 GN=ASCRUDRAFT_29311 PE=3 SV=1
LL1 pKa = 5.9QRR3 pKa = 11.84RR4 pKa = 11.84WKK6 pKa = 10.81ARR8 pKa = 11.84GNTYY12 pKa = 10.27QPSTLKK18 pKa = 10.25RR19 pKa = 11.84KK20 pKa = 9.45RR21 pKa = 11.84RR22 pKa = 11.84FGFFARR28 pKa = 11.84MTSRR32 pKa = 11.84TGRR35 pKa = 11.84KK36 pKa = 8.28IMQRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84SKK44 pKa = 10.3GRR46 pKa = 11.84WYY48 pKa = 9.14LTHH51 pKa = 7.23

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6733

2

6735

3141677

49

4919

466.5

53.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.976 ± 0.029

1.087 ± 0.01

5.919 ± 0.024

5.585 ± 0.031

5.019 ± 0.025

3.945 ± 0.028

1.876 ± 0.01

8.163 ± 0.028

8.142 ± 0.039

9.844 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.46 ± 0.009

10.668 ± 0.075

3.77 ± 0.022

3.683 ± 0.028

3.384 ± 0.018

9.663 ± 0.039

5.027 ± 0.023

4.308 ± 0.025

0.8 ± 0.01

3.678 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski