Wuhan house centipede virus 1
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KKG1|A0A1L3KKG1_9VIRU Non-structural protein 3 OS=Wuhan house centipede virus 1 OX=1923705 PE=4 SV=1
MM1 pKa = 7.32 YY2 pKa = 10.36 IKK4 pKa = 10.56 FCVLLYY10 pKa = 9.95 FVSFVNSAQLYY21 pKa = 9.49 SRR23 pKa = 11.84 WLEE26 pKa = 3.61 NGYY29 pKa = 10.47 FGTNITLPLLSLKK42 pKa = 9.31 YY43 pKa = 9.08 TEE45 pKa = 4.76 TPVGQYY51 pKa = 10.15 SRR53 pKa = 11.84 SPFVFEE59 pKa = 4.44 KK60 pKa = 10.82 SCISVVKK67 pKa = 9.99 TNTLLEE73 pKa = 4.24 VDD75 pKa = 4.16 CQLPQHH81 pKa = 6.53 CGVLHH86 pKa = 6.03 HH87 pKa = 7.16 RR88 pKa = 11.84 KK89 pKa = 8.24 MPQGWFGQEE98 pKa = 3.85 VILCYY103 pKa = 10.45 SFHH106 pKa = 5.66 QTTLGGALKK115 pKa = 10.55 VSTMDD120 pKa = 3.79 FSPSSFTYY128 pKa = 9.85 EE129 pKa = 4.25 SPNGPLTVEE138 pKa = 4.47 DD139 pKa = 3.72 WTSVDD144 pKa = 4.29 FFSHH148 pKa = 6.9 VYY150 pKa = 10.4 LLNSNDD156 pKa = 3.81 RR157 pKa = 11.84 YY158 pKa = 11.2 SLIPRR163 pKa = 11.84 ICLDD167 pKa = 4.12 HH168 pKa = 5.96 YY169 pKa = 10.01 TSSNDD174 pKa = 3.58 DD175 pKa = 3.41 QPQSVQFEE183 pKa = 4.29 FTEE186 pKa = 4.34 SQVCVHH192 pKa = 6.06 QSLHH196 pKa = 6.84 PEE198 pKa = 4.14 TDD200 pKa = 4.73 CEE202 pKa = 4.4 PEE204 pKa = 3.87 WYY206 pKa = 10.1 EE207 pKa = 4.61 SMEE210 pKa = 4.07 KK211 pKa = 10.7 LIVSYY216 pKa = 9.84 DD217 pKa = 3.41 YY218 pKa = 11.0 PVAYY222 pKa = 10.09 RR223 pKa = 11.84 GNPYY227 pKa = 10.68 EE228 pKa = 4.25 LVTHH232 pKa = 6.48 IRR234 pKa = 11.84 YY235 pKa = 9.67 GNQDD239 pKa = 3.72 PNVINYY245 pKa = 8.73 VSSNKK250 pKa = 9.59 LNHH253 pKa = 5.72 VFSVIDD259 pKa = 3.71 GKK261 pKa = 9.46 TYY263 pKa = 10.7 SSAVGLSHH271 pKa = 7.08 MYY273 pKa = 10.51 LHH275 pKa = 7.42 GISSPEE281 pKa = 3.81 NYY283 pKa = 9.13 IVLVDD288 pKa = 4.14 PSPLKK293 pKa = 10.86 VNQSFCYY300 pKa = 9.79 PIQSVYY306 pKa = 10.88 KK307 pKa = 9.13 NPLSAVINSIIDD319 pKa = 3.39 ALEE322 pKa = 4.4 PIILKK327 pKa = 10.18 LLSILEE333 pKa = 4.07 QYY335 pKa = 10.57 LEE337 pKa = 4.19 KK338 pKa = 10.4 FLKK341 pKa = 10.45 IFIKK345 pKa = 10.76 ALLIFLKK352 pKa = 10.61 LFYY355 pKa = 10.77 EE356 pKa = 5.33 LIDD359 pKa = 3.68 NLLTQEE365 pKa = 4.9 IITALLCSAAVYY377 pKa = 9.92 LYY379 pKa = 10.64 FKK381 pKa = 10.44 DD382 pKa = 3.95 YY383 pKa = 11.14 VITFISFCLFCFLLYY398 pKa = 10.85 YY399 pKa = 10.22 IFF401 pKa = 5.33
Molecular weight: 46.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.319
IPC2_protein 5.181
IPC_protein 5.156
Toseland 5.283
ProMoST 5.372
Dawson 5.245
Bjellqvist 5.334
Wikipedia 5.169
Rodwell 5.194
Grimsley 5.283
Solomon 5.245
Lehninger 5.219
Nozaki 5.423
DTASelect 5.575
Thurlkill 5.372
EMBOSS 5.334
Sillero 5.512
Patrickios 0.337
IPC_peptide 5.258
IPC2_peptide 5.512
IPC2.peptide.svr19 5.343
Protein with the highest isoelectric point:
>tr|A0A1L3KKD7|A0A1L3KKD7_9VIRU DiSB-ORF2_chro domain-containing protein OS=Wuhan house centipede virus 1 OX=1923705 PE=4 SV=1
MM1 pKa = 7.39 SSAVRR6 pKa = 11.84 NRR8 pKa = 11.84 SRR10 pKa = 11.84 IPVVSQRR17 pKa = 11.84 TVQRR21 pKa = 11.84 PRR23 pKa = 11.84 VVTRR27 pKa = 11.84 TRR29 pKa = 11.84 VVVPRR34 pKa = 11.84 QKK36 pKa = 9.46 TRR38 pKa = 11.84 NTPRR42 pKa = 11.84 SNYY45 pKa = 9.49 FDD47 pKa = 4.14 LNKK50 pKa = 9.82 VFTDD54 pKa = 3.59 ISSTFTRR61 pKa = 11.84 ALSNPLVLCTISIALGVILTHH82 pKa = 7.55 DD83 pKa = 3.94 FEE85 pKa = 5.25 KK86 pKa = 11.06 KK87 pKa = 9.94 NGYY90 pKa = 9.84 IYY92 pKa = 9.59 DD93 pKa = 3.91 TFKK96 pKa = 11.05 EE97 pKa = 4.19 RR98 pKa = 11.84 NDD100 pKa = 4.84 NISQWIVNNGKK111 pKa = 9.59 KK112 pKa = 9.93 FAGLLIFAPAVVDD125 pKa = 4.03 APRR128 pKa = 11.84 NLRR131 pKa = 11.84 AVVALVSFLWVMIIPEE147 pKa = 4.31 SKK149 pKa = 9.13 AAEE152 pKa = 4.1 YY153 pKa = 10.19 FLQSLALHH161 pKa = 6.44 TYY163 pKa = 9.85 FRR165 pKa = 11.84 LSNNNSKK172 pKa = 10.8 LMVLFMVVIAWFLGFLTVGKK192 pKa = 10.22 NSSS195 pKa = 3.04
Molecular weight: 22.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.94
IPC_protein 10.877
Toseland 10.994
ProMoST 10.833
Dawson 11.067
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.242
Grimsley 11.111
Solomon 11.257
Lehninger 11.213
Nozaki 10.965
DTASelect 10.818
Thurlkill 10.979
EMBOSS 11.403
Sillero 11.008
Patrickios 10.965
IPC_peptide 11.257
IPC2_peptide 9.838
IPC2.peptide.svr19 8.406
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3135
166
2373
783.8
89.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.402 ± 0.54
2.711 ± 0.363
5.04 ± 0.682
5.71 ± 1.092
5.423 ± 0.605
3.7 ± 0.211
2.679 ± 0.256
6.252 ± 0.234
5.391 ± 0.552
9.601 ± 0.884
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.285
5.837 ± 0.582
4.402 ± 0.265
3.126 ± 0.307
5.136 ± 0.898
8.995 ± 0.459
5.678 ± 0.203
7.719 ± 0.729
0.574 ± 0.396
5.646 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here