Ajellomyces dermatitidis (strain ER-3 / ATCC MYA-2586) (Blastomyces dermatitidis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Blastomyces; Blastomyces dermatitidis

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179U3Q3|A0A179U3Q3_AJEDR Uncharacterized protein OS=Ajellomyces dermatitidis (strain ER-3 / ATCC MYA-2586) OX=559297 GN=BDCG_17296 PE=4 SV=1
MM1 pKa = 7.49NLQDD5 pKa = 5.74DD6 pKa = 4.45ICTHH10 pKa = 5.25YY11 pKa = 9.63HH12 pKa = 5.36TVDD15 pKa = 3.5LRR17 pKa = 11.84QLSDD21 pKa = 2.88KK22 pKa = 11.17SFLFSQEE29 pKa = 3.97NNLDD33 pKa = 3.96LSDD36 pKa = 5.1LDD38 pKa = 3.6SDD40 pKa = 5.04LPVLSQVEE48 pKa = 4.61EE49 pKa = 3.87ILISHH54 pKa = 5.79MHH56 pKa = 5.24VFIEE60 pKa = 4.17LSEE63 pKa = 4.5NEE65 pKa = 4.6DD66 pKa = 3.21VSDD69 pKa = 3.75QIPFHH74 pKa = 6.67EE75 pKa = 5.08LDD77 pKa = 3.6KK78 pKa = 11.67QEE80 pKa = 3.96LTVIEE85 pKa = 4.22NEE87 pKa = 4.18NYY89 pKa = 10.88LNLDD93 pKa = 3.62NLNISPEE100 pKa = 4.01YY101 pKa = 10.88SDD103 pKa = 5.41VSDD106 pKa = 4.86LLISQFKK113 pKa = 10.61VEE115 pKa = 3.98QLHH118 pKa = 6.0YY119 pKa = 10.2EE120 pKa = 4.15LEE122 pKa = 4.33ISQQ125 pKa = 3.63

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5GY47|C5GY47_AJEDR Hydantoinase OS=Ajellomyces dermatitidis (strain ER-3 / ATCC MYA-2586) OX=559297 GN=BDCG_09424 PE=4 SV=1
MM1 pKa = 7.26SHH3 pKa = 6.29QNPGIIRR10 pKa = 11.84MVAGGTFYY18 pKa = 11.0SRR20 pKa = 11.84KK21 pKa = 9.35SSSIYY26 pKa = 9.77TSLGCRR32 pKa = 11.84QSVRR36 pKa = 11.84GQHH39 pKa = 6.46KK40 pKa = 8.99KK41 pKa = 7.06TQHH44 pKa = 5.14ACKK47 pKa = 10.18AHH49 pKa = 5.0TLCLEE54 pKa = 3.68SRR56 pKa = 11.84EE57 pKa = 4.62AYY59 pKa = 7.33TASPEE64 pKa = 3.85GRR66 pKa = 11.84RR67 pKa = 11.84TCSSTSHH74 pKa = 6.04VGNPSSFTLRR84 pKa = 11.84RR85 pKa = 11.84ARR87 pKa = 11.84GEE89 pKa = 3.79EE90 pKa = 3.87RR91 pKa = 11.84KK92 pKa = 10.4AGILKK97 pKa = 10.33LRR99 pKa = 11.84GPDD102 pKa = 3.04QDD104 pKa = 3.02RR105 pKa = 11.84GKK107 pKa = 10.06RR108 pKa = 11.84FSGAQPDD115 pKa = 4.21HH116 pKa = 6.6EE117 pKa = 4.64MSLALTRR124 pKa = 4.18

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9745

653

10398

4587642

36

5907

441.2

48.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.948 ± 0.021

1.224 ± 0.009

5.566 ± 0.019

6.262 ± 0.025

3.612 ± 0.016

6.716 ± 0.024

2.493 ± 0.011

5.042 ± 0.018

5.035 ± 0.022

8.764 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.009

3.925 ± 0.012

6.333 ± 0.028

4.105 ± 0.017

6.419 ± 0.022

8.794 ± 0.029

5.967 ± 0.017

5.725 ± 0.016

1.301 ± 0.009

2.644 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski