Calditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1)
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2089 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E4TJG6|E4TJG6_CALNY Enoyl-CoA hydratase/isomerase OS=Calditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1) OX=768670 GN=Calni_1325 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 6.11 PSFCPYY8 pKa = 10.17 CGEE11 pKa = 4.02 EE12 pKa = 4.78 HH13 pKa = 7.3 LDD15 pKa = 3.62 EE16 pKa = 5.63 LDD18 pKa = 3.55 PTEE21 pKa = 5.94 LMVDD25 pKa = 3.39 NQKK28 pKa = 10.52 WIIYY32 pKa = 9.49 HH33 pKa = 5.44 YY34 pKa = 8.35 EE35 pKa = 4.01 CKK37 pKa = 10.56 VCGEE41 pKa = 4.46 IFDD44 pKa = 5.02 KK45 pKa = 10.82 IYY47 pKa = 10.4 IDD49 pKa = 3.78 EE50 pKa = 4.95 EE51 pKa = 4.47 YY52 pKa = 11.29 GDD54 pKa = 4.3 MEE56 pKa = 6.61 DD57 pKa = 6.47 DD58 pKa = 3.79 EE59 pKa = 7.14 DD60 pKa = 5.59 DD61 pKa = 4.82 EE62 pKa = 4.6 NRR64 pKa = 11.84 LWSS67 pKa = 3.62
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.834
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.528
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.439
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.834
Patrickios 0.248
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.756
Protein with the highest isoelectric point:
>tr|E4TI08|E4TI08_CALNY Putative membrane protein insertion efficiency factor OS=Calditerrivibrio nitroreducens (strain DSM 19672 / NBRC 101217 / Yu37-1) OX=768670 GN=Calni_1027 PE=3 SV=1
MM1 pKa = 7.24 AQMRR5 pKa = 11.84 TLKK8 pKa = 10.79 KK9 pKa = 9.15 LTKK12 pKa = 9.5 LKK14 pKa = 10.39 KK15 pKa = 9.98 IRR17 pKa = 11.84 DD18 pKa = 3.38 HH19 pKa = 6.51 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 KK25 pKa = 6.71 STKK28 pKa = 9.74 GGRR31 pKa = 11.84 NVLKK35 pKa = 10.44 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 9.91 GRR42 pKa = 11.84 KK43 pKa = 8.85 RR44 pKa = 11.84 LALL47 pKa = 3.85
Molecular weight: 5.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.179
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2089
0
2089
683502
32
1706
327.2
37.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.549 ± 0.059
0.927 ± 0.024
5.855 ± 0.04
6.915 ± 0.047
5.388 ± 0.05
6.342 ± 0.056
1.48 ± 0.021
9.783 ± 0.061
9.033 ± 0.049
9.524 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.024
5.386 ± 0.053
3.218 ± 0.03
2.237 ± 0.021
3.766 ± 0.031
6.155 ± 0.037
4.6 ± 0.035
6.584 ± 0.044
0.659 ± 0.016
4.251 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here