Prevotella sp. CAG:732

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6X9E9|R6X9E9_9BACT Site-specific DNA-methyltransferase (adenine-specific) OS=Prevotella sp. CAG:732 OX=1262934 GN=BN769_01573 PE=3 SV=1
MM1 pKa = 7.41KK2 pKa = 10.04KK3 pKa = 10.07IYY5 pKa = 7.36MTPHH9 pKa = 7.52LEE11 pKa = 3.48ILAVVNSEE19 pKa = 4.03QLCDD23 pKa = 3.67GGGLYY28 pKa = 10.35GWSATNEE35 pKa = 4.13SDD37 pKa = 3.77GSSDD41 pKa = 4.25GGGSSDD47 pKa = 4.68NDD49 pKa = 3.64GDD51 pKa = 4.5PEE53 pKa = 4.3WGTAGAKK60 pKa = 10.34HH61 pKa = 5.87FDD63 pKa = 4.27CWNSWDD69 pKa = 3.9DD70 pKa = 3.42

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6XBM2|R6XBM2_9BACT Uncharacterized protein OS=Prevotella sp. CAG:732 OX=1262934 GN=BN769_01886 PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2409

0

2409

864167

29

2859

358.7

40.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.263 ± 0.038

1.304 ± 0.017

5.954 ± 0.037

6.302 ± 0.048

4.391 ± 0.031

6.911 ± 0.042

2.09 ± 0.022

6.541 ± 0.043

7.132 ± 0.037

8.654 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.98 ± 0.027

5.237 ± 0.048

3.518 ± 0.026

3.657 ± 0.025

4.174 ± 0.031

6.08 ± 0.042

5.616 ± 0.046

6.45 ± 0.038

1.34 ± 0.018

4.402 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski