Amino acid dipepetide frequency for Prevotella sp. CAG:732

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.597AlaAla: 5.597 ± 0.11	1.028AlaCys: 1.028 ± 0.035	4.423AlaAsp: 4.423 ± 0.08	4.704AlaGlu: 4.704 ± 0.086	3.152AlaPhe: 3.152 ± 0.062	4.722AlaGly: 4.722 ± 0.092	1.415AlaHis: 1.415 ± 0.04	4.955AlaIle: 4.955 ± 0.099	5.252AlaLys: 5.252 ± 0.088	6.505AlaLeu: 6.505 ± 0.103	2.252AlaMet: 2.252 ± 0.058	3.785AlaAsn: 3.785 ± 0.083	2.233AlaPro: 2.233 ± 0.052	3.014AlaGln: 3.014 ± 0.074	2.733AlaArg: 2.733 ± 0.064	4.609AlaSer: 4.609 ± 0.084	4.048AlaThr: 4.048 ± 0.074	4.227AlaVal: 4.227 ± 0.079	0.972AlaTrp: 0.972 ± 0.038	3.006AlaTyr: 3.006 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.778CysAla: 0.778 ± 0.031	0.229CysCys: 0.229 ± 0.014	0.782CysAsp: 0.782 ± 0.031	0.739CysGlu: 0.739 ± 0.028	0.613CysPhe: 0.613 ± 0.022	1.121CysGly: 1.121 ± 0.04	0.355CysHis: 0.355 ± 0.022	0.883CysIle: 0.883 ± 0.036	0.794CysLys: 0.794 ± 0.03	1.198CysLeu: 1.198 ± 0.04	0.365CysMet: 0.365 ± 0.02	0.606CysAsn: 0.606 ± 0.028	0.552CysPro: 0.552 ± 0.03	0.451CysGln: 0.451 ± 0.025	0.579CysArg: 0.579 ± 0.027	0.827CysSer: 0.827 ± 0.029	0.633CysThr: 0.633 ± 0.029	0.781CysVal: 0.781 ± 0.032	0.171CysTrp: 0.171 ± 0.016	0.587CysTyr: 0.587 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.646AspAla: 4.646 ± 0.077	0.691AspCys: 0.691 ± 0.029	3.48AspAsp: 3.48 ± 0.083	4.348AspGlu: 4.348 ± 0.072	3.148AspPhe: 3.148 ± 0.06	4.769AspGly: 4.769 ± 0.089	1.117AspHis: 1.117 ± 0.035	4.173AspIle: 4.173 ± 0.072	4.382AspLys: 4.382 ± 0.086	4.584AspLeu: 4.584 ± 0.074	1.742AspMet: 1.742 ± 0.048	3.043AspAsn: 3.043 ± 0.062	1.868AspPro: 1.868 ± 0.05	1.555AspGln: 1.555 ± 0.043	2.231AspArg: 2.231 ± 0.055	3.122AspSer: 3.122 ± 0.067	2.755AspThr: 2.755 ± 0.06	3.959AspVal: 3.959 ± 0.079	0.951AspTrp: 0.951 ± 0.034	2.976AspTyr: 2.976 ± 0.056	0.001AspXaa: 0.001 ± 0.001
Glu	4.704GluAla: 4.704 ± 0.079	0.739GluCys: 0.739 ± 0.032	3.616GluAsp: 3.616 ± 0.078	4.969GluGlu: 4.969 ± 0.086	2.366GluPhe: 2.366 ± 0.053	4.246GluGly: 4.246 ± 0.074	1.35GluHis: 1.35 ± 0.044	4.03GluIle: 4.03 ± 0.076	5.089GluLys: 5.089 ± 0.087	5.173GluLeu: 5.173 ± 0.092	2.163GluMet: 2.163 ± 0.051	3.381GluAsn: 3.381 ± 0.067	1.772GluPro: 1.772 ± 0.042	2.62GluGln: 2.62 ± 0.058	2.856GluArg: 2.856 ± 0.061	3.057GluSer: 3.057 ± 0.059	3.133GluThr: 3.133 ± 0.066	4.198GluVal: 4.198 ± 0.069	0.881GluTrp: 0.881 ± 0.031	2.672GluTyr: 2.672 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	3.154PheAla: 3.154 ± 0.065	0.77PheCys: 0.77 ± 0.031	2.988PheAsp: 2.988 ± 0.052	2.366PheGlu: 2.366 ± 0.05	2.042PhePhe: 2.042 ± 0.063	3.341PheGly: 3.341 ± 0.074	0.958PheHis: 0.958 ± 0.031	2.748PheIle: 2.748 ± 0.06	2.528PheLys: 2.528 ± 0.055	3.728PheLeu: 3.728 ± 0.076	1.177PheMet: 1.177 ± 0.037	2.215PheAsn: 2.215 ± 0.052	1.474PhePro: 1.474 ± 0.041	1.325PheGln: 1.325 ± 0.041	1.883PheArg: 1.883 ± 0.05	3.212PheSer: 3.212 ± 0.069	2.709PheThr: 2.709 ± 0.065	2.886PheVal: 2.886 ± 0.062	0.553PheTrp: 0.553 ± 0.028	1.851PheTyr: 1.851 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.482GlyAla: 4.482 ± 0.089	0.994GlyCys: 0.994 ± 0.034	3.859GlyAsp: 3.859 ± 0.08	4.294GlyGlu: 4.294 ± 0.072	3.111GlyPhe: 3.111 ± 0.063	4.717GlyGly: 4.717 ± 0.112	1.273GlyHis: 1.273 ± 0.04	5.215GlyIle: 5.215 ± 0.091	6.182GlyLys: 6.182 ± 0.074	5.546GlyLeu: 5.546 ± 0.09	2.182GlyMet: 2.182 ± 0.05	3.814GlyAsn: 3.814 ± 0.09	1.267GlyPro: 1.267 ± 0.04	2.061GlyGln: 2.061 ± 0.057	2.627GlyArg: 2.627 ± 0.058	4.165GlySer: 4.165 ± 0.094	4.223GlyThr: 4.223 ± 0.095	4.726GlyVal: 4.726 ± 0.077	1.081GlyTrp: 1.081 ± 0.043	3.295GlyTyr: 3.295 ± 0.076	0.0GlyXaa: 0.0 ± 0.0
His	1.28HisAla: 1.28 ± 0.035	0.33HisCys: 0.33 ± 0.022	1.186HisAsp: 1.186 ± 0.038	1.15HisGlu: 1.15 ± 0.038	1.094HisPhe: 1.094 ± 0.044	1.382HisGly: 1.382 ± 0.045	0.668HisHis: 0.668 ± 0.032	1.539HisIle: 1.539 ± 0.042	1.106HisLys: 1.106 ± 0.038	2.063HisLeu: 2.063 ± 0.063	0.351HisMet: 0.351 ± 0.022	1.054HisAsn: 1.054 ± 0.039	1.029HisPro: 1.029 ± 0.035	0.832HisGln: 0.832 ± 0.033	1.009HisArg: 1.009 ± 0.034	1.215HisSer: 1.215 ± 0.036	1.099HisThr: 1.099 ± 0.038	1.279HisVal: 1.279 ± 0.041	0.264HisTrp: 0.264 ± 0.016	0.966HisTyr: 0.966 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.074IleAla: 5.074 ± 0.084	0.98IleCys: 0.98 ± 0.036	4.515IleAsp: 4.515 ± 0.078	4.039IleGlu: 4.039 ± 0.079	2.607IlePhe: 2.607 ± 0.07	4.422IleGly: 4.422 ± 0.089	1.389IleHis: 1.389 ± 0.045	4.309IleIle: 4.309 ± 0.09	4.343IleLys: 4.343 ± 0.07	5.386IleLeu: 5.386 ± 0.103	1.544IleMet: 1.544 ± 0.04	3.359IleAsn: 3.359 ± 0.064	2.782IlePro: 2.782 ± 0.058	2.281IleGln: 2.281 ± 0.047	2.754IleArg: 2.754 ± 0.06	4.18IleSer: 4.18 ± 0.078	3.888IleThr: 3.888 ± 0.076	4.221IleVal: 4.221 ± 0.076	0.712IleTrp: 0.712 ± 0.03	2.624IleTyr: 2.624 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	5.41LysAla: 5.41 ± 0.092	0.643LysCys: 0.643 ± 0.029	4.501LysAsp: 4.501 ± 0.085	5.37LysGlu: 5.37 ± 0.088	2.424LysPhe: 2.424 ± 0.049	4.931LysGly: 4.931 ± 0.078	1.408LysHis: 1.408 ± 0.039	3.958LysIle: 3.958 ± 0.078	5.71LysLys: 5.71 ± 0.108	5.536LysLeu: 5.536 ± 0.078	2.627LysMet: 2.627 ± 0.056	3.923LysAsn: 3.923 ± 0.062	2.267LysPro: 2.267 ± 0.052	2.749LysGln: 2.749 ± 0.063	3.061LysArg: 3.061 ± 0.062	3.803LysSer: 3.803 ± 0.064	3.969LysThr: 3.969 ± 0.074	4.861LysVal: 4.861 ± 0.073	0.964LysTrp: 0.964 ± 0.036	3.202LysTyr: 3.202 ± 0.062	0.001LysXaa: 0.001 ± 0.001
Leu	6.53LeuAla: 6.53 ± 0.092	1.313LeuCys: 1.313 ± 0.043	4.942LeuAsp: 4.942 ± 0.09	4.589LeuGlu: 4.589 ± 0.08	3.792LeuPhe: 3.792 ± 0.08	5.787LeuGly: 5.787 ± 0.102	1.995LeuHis: 1.995 ± 0.058	4.747LeuIle: 4.747 ± 0.099	6.12LeuLys: 6.12 ± 0.088	7.776LeuLeu: 7.776 ± 0.144	2.601LeuMet: 2.601 ± 0.059	4.317LeuAsn: 4.317 ± 0.078	3.656LeuPro: 3.656 ± 0.068	3.356LeuGln: 3.356 ± 0.063	3.925LeuArg: 3.925 ± 0.073	6.103LeuSer: 6.103 ± 0.102	5.143LeuThr: 5.143 ± 0.085	5.332LeuVal: 5.332 ± 0.091	1.073LeuTrp: 1.073 ± 0.037	3.445LeuTyr: 3.445 ± 0.078	0.001LeuXaa: 0.001 ± 0.001
Met	2.523MetAla: 2.523 ± 0.059	0.259MetCys: 0.259 ± 0.02	1.678MetAsp: 1.678 ± 0.046	1.893MetGlu: 1.893 ± 0.047	0.957MetPhe: 0.957 ± 0.034	2.104MetGly: 2.104 ± 0.049	0.524MetHis: 0.524 ± 0.025	1.646MetIle: 1.646 ± 0.051	2.681MetLys: 2.681 ± 0.054	2.543MetLeu: 2.543 ± 0.064	1.065MetMet: 1.065 ± 0.035	1.611MetAsn: 1.611 ± 0.048	1.268MetPro: 1.268 ± 0.036	1.165MetGln: 1.165 ± 0.037	1.325MetArg: 1.325 ± 0.046	1.77MetSer: 1.77 ± 0.046	1.768MetThr: 1.768 ± 0.041	1.854MetVal: 1.854 ± 0.051	0.299MetTrp: 0.299 ± 0.021	0.868MetTyr: 0.868 ± 0.031	0.001MetXaa: 0.001 ± 0.001
Asn	3.918AsnAla: 3.918 ± 0.075	0.536AsnCys: 0.536 ± 0.026	2.839AsnAsp: 2.839 ± 0.063	3.02AsnGlu: 3.02 ± 0.055	2.325AsnPhe: 2.325 ± 0.052	4.205AsnGly: 4.205 ± 0.097	1.104AsnHis: 1.104 ± 0.036	3.765AsnIle: 3.765 ± 0.069	3.385AsnLys: 3.385 ± 0.069	4.411AsnLeu: 4.411 ± 0.072	1.413AsnMet: 1.413 ± 0.041	2.734AsnAsn: 2.734 ± 0.071	2.472AsnPro: 2.472 ± 0.058	1.897AsnGln: 1.897 ± 0.05	2.144AsnArg: 2.144 ± 0.053	2.807AsnSer: 2.807 ± 0.058	2.724AsnThr: 2.724 ± 0.068	3.517AsnVal: 3.517 ± 0.065	0.693AsnTrp: 0.693 ± 0.032	2.464AsnTyr: 2.464 ± 0.063	0.001AsnXaa: 0.001 ± 0.001
Pro	2.519ProAla: 2.519 ± 0.06	0.382ProCys: 0.382 ± 0.02	2.239ProAsp: 2.239 ± 0.055	2.852ProGlu: 2.852 ± 0.061	1.733ProPhe: 1.733 ± 0.045	2.118ProGly: 2.118 ± 0.054	0.712ProHis: 0.712 ± 0.029	2.282ProIle: 2.282 ± 0.05	2.208ProLys: 2.208 ± 0.048	2.909ProLeu: 2.909 ± 0.067	1.046ProMet: 1.046 ± 0.031	1.788ProAsn: 1.788 ± 0.048	0.605ProPro: 0.605 ± 0.028	1.401ProGln: 1.401 ± 0.045	1.209ProArg: 1.209 ± 0.037	2.299ProSer: 2.299 ± 0.054	2.266ProThr: 2.266 ± 0.05	2.468ProVal: 2.468 ± 0.058	0.466ProTrp: 0.466 ± 0.025	1.672ProTyr: 1.672 ± 0.052	0.001ProXaa: 0.001 ± 0.001
Gln	2.741GlnAla: 2.741 ± 0.054	0.354GlnCys: 0.354 ± 0.021	1.799GlnAsp: 1.799 ± 0.048	2.395GlnGlu: 2.395 ± 0.063	1.437GlnPhe: 1.437 ± 0.04	2.295GlnGly: 2.295 ± 0.064	0.787GlnHis: 0.787 ± 0.034	2.262GlnIle: 2.262 ± 0.05	2.708GlnLys: 2.708 ± 0.059	3.544GlnLeu: 3.544 ± 0.059	1.305GlnMet: 1.305 ± 0.039	1.885GlnAsn: 1.885 ± 0.045	1.259GlnPro: 1.259 ± 0.04	1.774GlnGln: 1.774 ± 0.051	1.635GlnArg: 1.635 ± 0.047	1.865GlnSer: 1.865 ± 0.045	2.008GlnThr: 2.008 ± 0.048	2.456GlnVal: 2.456 ± 0.056	0.502GlnTrp: 0.502 ± 0.028	1.558GlnTyr: 1.558 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.457ArgAla: 2.457 ± 0.067	0.472ArgCys: 0.472 ± 0.024	2.125ArgAsp: 2.125 ± 0.048	2.547ArgGlu: 2.547 ± 0.059	2.073ArgPhe: 2.073 ± 0.053	2.424ArgGly: 2.424 ± 0.059	0.977ArgHis: 0.977 ± 0.036	3.073ArgIle: 3.073 ± 0.062	3.238ArgLys: 3.238 ± 0.068	3.971ArgLeu: 3.971 ± 0.077	1.456ArgMet: 1.456 ± 0.04	2.39ArgAsn: 2.39 ± 0.059	1.418ArgPro: 1.418 ± 0.039	1.845ArgGln: 1.845 ± 0.052	2.017ArgArg: 2.017 ± 0.054	2.147ArgSer: 2.147 ± 0.052	2.045ArgThr: 2.045 ± 0.054	2.401ArgVal: 2.401 ± 0.05	0.64ArgTrp: 0.64 ± 0.027	2.026ArgTyr: 2.026 ± 0.053	0.002ArgXaa: 0.002 ± 0.002
Ser	4.164SerAla: 4.164 ± 0.079	0.857SerCys: 0.857 ± 0.037	3.619SerAsp: 3.619 ± 0.063	3.379SerGlu: 3.379 ± 0.06	3.047SerPhe: 3.047 ± 0.07	4.175SerGly: 4.175 ± 0.093	1.308SerHis: 1.308 ± 0.041	4.093SerIle: 4.093 ± 0.08	3.848SerLys: 3.848 ± 0.07	5.786SerLeu: 5.786 ± 0.096	1.607SerMet: 1.607 ± 0.043	2.972SerAsn: 2.972 ± 0.07	2.185SerPro: 2.185 ± 0.05	2.151SerGln: 2.151 ± 0.054	2.415SerArg: 2.415 ± 0.051	4.169SerSer: 4.169 ± 0.095	3.304SerThr: 3.304 ± 0.071	3.936SerVal: 3.936 ± 0.074	0.875SerTrp: 0.875 ± 0.035	2.909SerTyr: 2.909 ± 0.073	0.0SerXaa: 0.0 ± 0.0
Thr	3.961ThrAla: 3.961 ± 0.077	0.66ThrCys: 0.66 ± 0.03	3.417ThrAsp: 3.417 ± 0.061	3.134ThrGlu: 3.134 ± 0.059	2.721ThrPhe: 2.721 ± 0.058	4.002ThrGly: 4.002 ± 0.084	1.131ThrHis: 1.131 ± 0.036	4.069ThrIle: 4.069 ± 0.066	3.204ThrLys: 3.204 ± 0.067	5.307ThrLeu: 5.307 ± 0.072	1.438ThrMet: 1.438 ± 0.037	2.525ThrAsn: 2.525 ± 0.068	2.686ThrPro: 2.686 ± 0.055	1.865ThrGln: 1.865 ± 0.049	2.007ThrArg: 2.007 ± 0.044	3.671ThrSer: 3.671 ± 0.075	3.457ThrThr: 3.457 ± 0.084	3.49ThrVal: 3.49 ± 0.066	0.793ThrTrp: 0.793 ± 0.034	2.626ThrTyr: 2.626 ± 0.063	0.0ThrXaa: 0.0 ± 0.0
Val	4.83ValAla: 4.83 ± 0.089	1.026ValCys: 1.026 ± 0.035	3.936ValAsp: 3.936 ± 0.072	4.121ValGlu: 4.121 ± 0.07	2.637ValPhe: 2.637 ± 0.057	4.247ValGly: 4.247 ± 0.09	1.041ValHis: 1.041 ± 0.037	4.212ValIle: 4.212 ± 0.073	4.959ValLys: 4.959 ± 0.082	5.435ValLeu: 5.435 ± 0.092	1.813ValMet: 1.813 ± 0.045	3.484ValAsn: 3.484 ± 0.07	2.413ValPro: 2.413 ± 0.06	1.783ValGln: 1.783 ± 0.042	2.688ValArg: 2.688 ± 0.055	4.326ValSer: 4.326 ± 0.081	3.789ValThr: 3.789 ± 0.083	4.579ValVal: 4.579 ± 0.082	0.741ValTrp: 0.741 ± 0.029	2.444ValTyr: 2.444 ± 0.05	0.001ValXaa: 0.001 ± 0.001
Trp	0.892TrpAla: 0.892 ± 0.034	0.189TrpCys: 0.189 ± 0.014	0.745TrpAsp: 0.745 ± 0.032	0.693TrpGlu: 0.693 ± 0.026	0.56TrpPhe: 0.56 ± 0.027	1.052TrpGly: 1.052 ± 0.039	0.331TrpHis: 0.331 ± 0.018	0.764TrpIle: 0.764 ± 0.033	0.997TrpLys: 0.997 ± 0.038	1.239TrpLeu: 1.239 ± 0.045	0.52TrpMet: 0.52 ± 0.026	0.94TrpAsn: 0.94 ± 0.034	0.275TrpPro: 0.275 ± 0.018	0.608TrpGln: 0.608 ± 0.023	0.57TrpArg: 0.57 ± 0.026	0.831TrpSer: 0.831 ± 0.034	0.713TrpThr: 0.713 ± 0.03	0.709TrpVal: 0.709 ± 0.029	0.205TrpTrp: 0.205 ± 0.015	0.57TrpTyr: 0.57 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.972TyrAla: 2.972 ± 0.063	0.592TyrCys: 0.592 ± 0.026	2.85TyrAsp: 2.85 ± 0.071	2.409TyrGlu: 2.409 ± 0.059	2.071TyrPhe: 2.071 ± 0.048	3.054TyrGly: 3.054 ± 0.076	1.053TyrHis: 1.053 ± 0.038	2.684TyrIle: 2.684 ± 0.062	2.585TyrLys: 2.585 ± 0.064	3.902TyrLeu: 3.902 ± 0.077	1.135TyrMet: 1.135 ± 0.036	2.547TyrAsn: 2.547 ± 0.064	1.698TyrPro: 1.698 ± 0.05	1.836TyrGln: 1.836 ± 0.044	2.073TyrArg: 2.073 ± 0.046	2.618TyrSer: 2.618 ± 0.07	2.489TyrThr: 2.489 ± 0.062	2.624TyrVal: 2.624 ± 0.052	0.569TyrTrp: 0.569 ± 0.028	2.257TyrTyr: 2.257 ± 0.068	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.014XaaXaa: 0.014 ± 0.006

Statistics based on 2409 proteins (864167 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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