Smithella sp. D17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Smithella; unclassified Smithella

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094K1Q2|A0A094K1Q2_9DELT Antitoxin OS=Smithella sp. D17 OX=1538639 GN=KD27_07510 PE=4 SV=1
MM1 pKa = 7.97AEE3 pKa = 3.67IKK5 pKa = 10.76KK6 pKa = 10.06PVTSEE11 pKa = 3.59MGHH14 pKa = 4.9QVTGKK19 pKa = 10.24VAILYY24 pKa = 9.89GNVKK28 pKa = 10.3AISPDD33 pKa = 2.91GTVRR37 pKa = 11.84MLKK40 pKa = 10.09INSPVFADD48 pKa = 4.05DD49 pKa = 5.01RR50 pKa = 11.84IITGGDD56 pKa = 3.2GSVSIVFNTSPSTQLDD72 pKa = 3.7LGRR75 pKa = 11.84VSDD78 pKa = 3.81MVIDD82 pKa = 3.74EE83 pKa = 4.4DD84 pKa = 4.74VYY86 pKa = 10.91GVVSKK91 pKa = 10.83GVAAEE96 pKa = 4.29ASAEE100 pKa = 3.9QEE102 pKa = 4.48AIQKK106 pKa = 10.5ALLAGDD112 pKa = 3.75QPLEE116 pKa = 4.25LDD118 pKa = 3.29ATAAGAEE125 pKa = 4.13ANAGGGHH132 pKa = 6.52PVFVVTPDD140 pKa = 2.98WSEE143 pKa = 3.85VTPEE147 pKa = 3.95SGAEE151 pKa = 3.85TRR153 pKa = 11.84GITWGTGVSTNYY165 pKa = 10.41DD166 pKa = 3.69PNLDD170 pKa = 3.64QPITVSISDD179 pKa = 4.48GLPDD183 pKa = 4.4PQSEE187 pKa = 4.5GQGGNVITFTVSLSEE202 pKa = 4.13ATNHH206 pKa = 6.14DD207 pKa = 3.42TTVNYY212 pKa = 7.33TTADD216 pKa = 3.45GTAVAGTDD224 pKa = 3.67YY225 pKa = 10.66IATSGTITFLAGEE238 pKa = 4.36TTATVTVPVLNDD250 pKa = 3.46TVFEE254 pKa = 4.16NPEE257 pKa = 3.92AFTVQLGTPSTGVTIVDD274 pKa = 3.94GSGIGNIADD283 pKa = 3.72NDD285 pKa = 3.94AVPAFSINDD294 pKa = 3.36VTVTEE299 pKa = 4.87GTDD302 pKa = 3.24STITFTVTKK311 pKa = 10.1TGLTDD316 pKa = 3.21QASSVDD322 pKa = 3.79YY323 pKa = 11.11SVAPNTAVTPGDD335 pKa = 3.68YY336 pKa = 8.95TASDD340 pKa = 4.32LLAGTLTFAAGEE352 pKa = 4.29TTKK355 pKa = 10.64TITLNIADD363 pKa = 4.16DD364 pKa = 3.86SVYY367 pKa = 11.07EE368 pKa = 3.97LTEE371 pKa = 4.01TFNVNLSNAAAATISDD387 pKa = 3.88TQGVGTILDD396 pKa = 4.21NDD398 pKa = 4.15DD399 pKa = 3.81TDD401 pKa = 3.69PTAGIIYY408 pKa = 8.22ATVDD412 pKa = 3.52DD413 pKa = 5.01EE414 pKa = 4.55GLSGGISGGTGDD426 pKa = 4.76IVVTPDD432 pKa = 3.64PDD434 pKa = 4.06SNEE437 pKa = 3.79ATFTGILTHH446 pKa = 7.22DD447 pKa = 4.43FVLNGPGSIGFASMNGLTGIVGQEE471 pKa = 3.83TVTYY475 pKa = 9.07SWTGSTLTATGPRR488 pKa = 11.84GALFTVEE495 pKa = 4.22VTDD498 pKa = 4.33PATGAYY504 pKa = 9.34KK505 pKa = 9.48VTLLDD510 pKa = 3.73NVLHH514 pKa = 6.07GTLDD518 pKa = 3.68GQVGDD523 pKa = 3.78NTEE526 pKa = 4.21NNATAALNYY535 pKa = 8.76TVTDD539 pKa = 3.84ADD541 pKa = 4.01GDD543 pKa = 4.31TATGTLNITFNDD555 pKa = 4.12DD556 pKa = 3.1MPTANDD562 pKa = 3.23QSVTVVVHH570 pKa = 7.03DD571 pKa = 4.23SATTAHH577 pKa = 6.97ISALAAGWVAPFDD590 pKa = 3.98INVDD594 pKa = 3.76TKK596 pKa = 10.81TSTDD600 pKa = 3.03ADD602 pKa = 4.02TYY604 pKa = 10.42FEE606 pKa = 4.73KK607 pKa = 10.61IAWGDD612 pKa = 3.49NSNSSSYY619 pKa = 11.17VYY621 pKa = 11.0NDD623 pKa = 3.01NNALATVNVGEE634 pKa = 4.45TFTLGTFTHH643 pKa = 6.54NNFPIPSGSSITDD656 pKa = 3.3AYY658 pKa = 11.27LKK660 pKa = 9.57VTFDD664 pKa = 3.79VVIDD668 pKa = 4.1GQTVHH673 pKa = 6.11VDD675 pKa = 3.2KK676 pKa = 10.63TVHH679 pKa = 5.58FQHH682 pKa = 6.9NEE684 pKa = 3.95TPNDD688 pKa = 3.68GANPDD693 pKa = 4.5DD694 pKa = 4.26IVTIVNGTNVVDD706 pKa = 4.01VKK708 pKa = 11.38VGDD711 pKa = 3.64FTYY714 pKa = 10.51TLTIGFQDD722 pKa = 3.2ASGNTVTQVYY732 pKa = 9.24TDD734 pKa = 3.67EE735 pKa = 4.55SAANTFNLNATLTADD750 pKa = 3.77ADD752 pKa = 4.04HH753 pKa = 7.04YY754 pKa = 10.54ILPVVGTVDD763 pKa = 3.22ASFGADD769 pKa = 3.25GPGALHH775 pKa = 6.53IVSIAHH781 pKa = 6.99DD782 pKa = 4.21ANGDD786 pKa = 4.02GIDD789 pKa = 3.6EE790 pKa = 4.61VYY792 pKa = 9.28NTSSGGYY799 pKa = 9.73NSTTTTLTIATHH811 pKa = 6.11EE812 pKa = 4.66GGTLAVNFSTGAYY825 pKa = 7.92TYY827 pKa = 7.37TAPTGTPLVANEE839 pKa = 3.93VFTYY843 pKa = 10.37TIMDD847 pKa = 4.24ADD849 pKa = 4.5GDD851 pKa = 4.17TATANLTVNLPVHH864 pKa = 6.89DD865 pKa = 4.16MLVVGTNADD874 pKa = 3.76DD875 pKa = 3.83VTGQTIDD882 pKa = 3.23HH883 pKa = 6.98HH884 pKa = 7.07IDD886 pKa = 3.03HH887 pKa = 6.94YY888 pKa = 11.61SPLDD892 pKa = 3.57GPITGGGGNDD902 pKa = 3.5VLVGDD907 pKa = 4.58IGGANNIVQPGQNYY921 pKa = 9.98NISLIVDD928 pKa = 3.83SSGSMDD934 pKa = 4.13KK935 pKa = 11.15DD936 pKa = 3.34SGTAGYY942 pKa = 8.38TRR944 pKa = 11.84MEE946 pKa = 4.46LAQAALKK953 pKa = 10.63NLADD957 pKa = 3.67QLVGHH962 pKa = 7.52DD963 pKa = 3.92GTINLQIIDD972 pKa = 3.84FDD974 pKa = 5.63SNVVANTSWSNITSANLSAIYY995 pKa = 8.71TAIDD999 pKa = 3.25NMVAEE1004 pKa = 4.9GATNYY1009 pKa = 8.82EE1010 pKa = 4.03AGFNASNAWLSSQTNGYY1027 pKa = 10.04EE1028 pKa = 3.98NITFFLTDD1036 pKa = 4.1GNPTYY1041 pKa = 10.78YY1042 pKa = 10.0INSYY1046 pKa = 10.85GNVAGPGKK1054 pKa = 9.3VTDD1057 pKa = 4.32YY1058 pKa = 10.4YY1059 pKa = 11.52TMLNSVEE1066 pKa = 4.61AFALLSPITHH1076 pKa = 6.09VEE1078 pKa = 4.08AIGIGSEE1085 pKa = 4.12VNEE1088 pKa = 4.29NYY1090 pKa = 10.79LKK1092 pKa = 10.9FFDD1095 pKa = 3.99NTDD1098 pKa = 3.03VTTEE1102 pKa = 3.95TGSVSFWNGTVTGPVGQVEE1121 pKa = 4.41IVHH1124 pKa = 5.6TAEE1127 pKa = 4.16EE1128 pKa = 4.04LSAALQHH1135 pKa = 6.8GSLEE1139 pKa = 4.2YY1140 pKa = 10.15TLLGAGDD1147 pKa = 4.08DD1148 pKa = 4.35HH1149 pKa = 6.83LASGDD1154 pKa = 3.54GDD1156 pKa = 3.94DD1157 pKa = 5.44VIFGDD1162 pKa = 5.18SVHH1165 pKa = 6.64TDD1167 pKa = 3.05ALADD1171 pKa = 4.11ANPSLTTADD1180 pKa = 3.48GAGWEE1185 pKa = 4.33VFEE1188 pKa = 4.34KK1189 pKa = 10.97LGWTEE1194 pKa = 3.87QQITDD1199 pKa = 4.06YY1200 pKa = 10.94IKK1202 pKa = 10.98ANHH1205 pKa = 5.87STLATEE1211 pKa = 4.55SGRR1214 pKa = 11.84TGGNDD1219 pKa = 3.69TIDD1222 pKa = 3.56GGAGNDD1228 pKa = 3.39IIYY1231 pKa = 9.65GQEE1234 pKa = 3.95GNDD1237 pKa = 3.65VINGGEE1243 pKa = 4.26GNDD1246 pKa = 4.03LLSGGTGNNILNGGLGADD1264 pKa = 3.45TFVISKK1270 pKa = 9.99GGHH1273 pKa = 5.73DD1274 pKa = 4.59TIQDD1278 pKa = 3.77YY1279 pKa = 11.58NKK1281 pKa = 10.6GQLDD1285 pKa = 3.73KK1286 pKa = 11.42VDD1288 pKa = 3.19ISSIVKK1294 pKa = 8.57VTDD1297 pKa = 3.44DD1298 pKa = 4.26AVAKK1302 pKa = 9.43TYY1304 pKa = 10.96LGVEE1308 pKa = 3.89QNANGKK1314 pKa = 9.48AVLDD1318 pKa = 4.04IYY1320 pKa = 11.61DD1321 pKa = 3.91NGSDD1325 pKa = 3.29HH1326 pKa = 7.29SAGHH1330 pKa = 6.72LVATVTFDD1338 pKa = 3.66NIHH1341 pKa = 6.05YY1342 pKa = 9.91SDD1344 pKa = 5.1LGSIHH1349 pKa = 6.51QLDD1352 pKa = 4.21SLLGAVDD1359 pKa = 3.91INHH1362 pKa = 6.23SS1363 pKa = 3.57

Molecular weight: 141.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094JX75|A0A094JX75_9DELT PCRF domain-containing protein (Fragment) OS=Smithella sp. D17 OX=1538639 GN=KD27_05320 PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 9.08KK3 pKa = 8.39TLTNKK8 pKa = 9.48TNTKK12 pKa = 9.77RR13 pKa = 11.84KK14 pKa = 7.29KK15 pKa = 7.47THH17 pKa = 5.51GFLVRR22 pKa = 11.84MASKK26 pKa = 10.38SGRR29 pKa = 11.84QVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.94GRR40 pKa = 11.84KK41 pKa = 8.66RR42 pKa = 11.84LAVV45 pKa = 3.41

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1343

0

1343

368400

41

1699

274.3

30.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.562 ± 0.063

1.138 ± 0.03

5.302 ± 0.044

6.45 ± 0.086

4.479 ± 0.06

6.814 ± 0.076

1.816 ± 0.029

8.217 ± 0.07

7.574 ± 0.079

9.472 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.03

4.51 ± 0.042

3.858 ± 0.034

3.2 ± 0.039

4.864 ± 0.058

6.044 ± 0.056

5.077 ± 0.066

6.543 ± 0.058

1.012 ± 0.028

3.357 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski