Streptomyces pseudovenezuelae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101N175|A0A101N175_9ACTN Regulator OS=Streptomyces pseudovenezuelae OX=67350 GN=AQI94_29245 PE=3 SV=1
MM1 pKa = 6.85WTPDD5 pKa = 3.42EE6 pKa = 4.52EE7 pKa = 4.18TDD9 pKa = 3.81EE10 pKa = 4.97ACDD13 pKa = 3.18AAYY16 pKa = 10.47DD17 pKa = 3.99KK18 pKa = 11.73GCVTIGGRR26 pKa = 11.84RR27 pKa = 11.84LHH29 pKa = 6.16TRR31 pKa = 11.84DD32 pKa = 4.31LDD34 pKa = 3.91ACTVTAYY41 pKa = 10.16EE42 pKa = 4.48LADD45 pKa = 4.36LADD48 pKa = 3.66QDD50 pKa = 5.44EE51 pKa = 4.5EE52 pKa = 4.19FDD54 pKa = 4.2PNGMEE59 pKa = 4.33SEE61 pKa = 4.29DD62 pKa = 3.55RR63 pKa = 11.84LVRR66 pKa = 11.84GDD68 pKa = 4.73LDD70 pKa = 4.11VALAWAAAGVCVVQQLLPYY89 pKa = 9.91PFGDD93 pKa = 3.53VLPYY97 pKa = 10.84GPIGNQPAHH106 pKa = 6.83SGRR109 pKa = 11.84AA110 pKa = 3.35

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101N3T1|A0A101N3T1_9ACTN GCN5 family acetyltransferase OS=Streptomyces pseudovenezuelae OX=67350 GN=AQI94_23270 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8204

0

8204

2728014

29

4971

332.5

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.265 ± 0.042

0.772 ± 0.008

6.052 ± 0.025

5.679 ± 0.031

2.782 ± 0.016

9.379 ± 0.026

2.31 ± 0.013

3.15 ± 0.019

2.217 ± 0.023

10.377 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.71 ± 0.011

1.842 ± 0.016

5.926 ± 0.02

2.823 ± 0.017

7.887 ± 0.03

5.171 ± 0.021

6.294 ± 0.021

8.595 ± 0.024

1.584 ± 0.012

2.185 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski