Avian orthoavulavirus 9
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0J7I1|C0J7I1_9MONO Phosphoprotein OS=Avian orthoavulavirus 9 OX=2560326 GN=P PE=2 SV=1
MM1 pKa = 7.53 SSIFNEE7 pKa = 4.23 YY8 pKa = 10.46 EE9 pKa = 4.02 SLLEE13 pKa = 4.17 SQLKK17 pKa = 7.98 PTGSNVLGEE26 pKa = 4.63 KK27 pKa = 10.64 GDD29 pKa = 3.86 TPKK32 pKa = 10.59 VEE34 pKa = 3.74 IPVFVLNSDD43 pKa = 3.66 NPEE46 pKa = 4.08 DD47 pKa = 3.28 RR48 pKa = 11.84 WNFTTFCLRR57 pKa = 11.84 VAVSEE62 pKa = 4.19 DD63 pKa = 3.04 ANRR66 pKa = 11.84 PLRR69 pKa = 11.84 QGALISLLCAHH80 pKa = 6.04 SQVMKK85 pKa = 10.38 NHH87 pKa = 5.4 VAIAGKK93 pKa = 9.41 QDD95 pKa = 3.04 EE96 pKa = 4.36 ALIVVLEE103 pKa = 4.28 IDD105 pKa = 4.35 TINDD109 pKa = 3.42 GVPAFNNRR117 pKa = 11.84 SGVTEE122 pKa = 3.81 EE123 pKa = 3.8 RR124 pKa = 11.84 AQRR127 pKa = 11.84 FAMIAQALPRR137 pKa = 11.84 ACANGTPFTVQDD149 pKa = 3.92 AEE151 pKa = 4.45 DD152 pKa = 5.05 DD153 pKa = 4.07 PVEE156 pKa = 5.46 DD157 pKa = 3.86 ITDD160 pKa = 3.76 ALDD163 pKa = 4.65 RR164 pKa = 11.84 ILSIQAQVWVTVAKK178 pKa = 10.87 SMTAYY183 pKa = 7.35 EE184 pKa = 4.18 TADD187 pKa = 3.22 EE188 pKa = 4.55 SEE190 pKa = 4.28 QKK192 pKa = 10.81 RR193 pKa = 11.84 LTKK196 pKa = 10.28 YY197 pKa = 7.97 VQQGRR202 pKa = 11.84 VQKK205 pKa = 10.66 KK206 pKa = 8.54 CMIYY210 pKa = 9.04 PVCRR214 pKa = 11.84 SMLQQIIRR222 pKa = 11.84 QSLAVRR228 pKa = 11.84 RR229 pKa = 11.84 FIVSEE234 pKa = 3.93 LKK236 pKa = 10.21 RR237 pKa = 11.84 ARR239 pKa = 11.84 NTAGGTSTYY248 pKa = 10.27 YY249 pKa = 11.15 NFVADD254 pKa = 3.44 VDD256 pKa = 4.31 SYY258 pKa = 11.5 IRR260 pKa = 11.84 NAGLTAFFLTLKK272 pKa = 10.55 YY273 pKa = 10.58 GVNTKK278 pKa = 9.96 TSVLALSSLAGDD290 pKa = 4.21 LQTVKK295 pKa = 10.81 QLMRR299 pKa = 11.84 LYY301 pKa = 10.14 KK302 pKa = 10.69 AKK304 pKa = 10.73 GDD306 pKa = 3.84 DD307 pKa = 3.88 APYY310 pKa = 8.26 MTILGDD316 pKa = 3.55 GDD318 pKa = 3.65 QMRR321 pKa = 11.84 FAPAEE326 pKa = 3.87 YY327 pKa = 10.11 AQLYY331 pKa = 8.96 SYY333 pKa = 11.23 AMGMASVIDD342 pKa = 4.19 KK343 pKa = 8.02 GTSRR347 pKa = 11.84 YY348 pKa = 9.45 QYY350 pKa = 11.43 ARR352 pKa = 11.84 DD353 pKa = 3.59 FLNPSFWRR361 pKa = 11.84 LGVEE365 pKa = 4.2 YY366 pKa = 10.49 AQTQGSNINEE376 pKa = 3.88 EE377 pKa = 4.19 MASEE381 pKa = 4.57 LKK383 pKa = 10.57 LSPIARR389 pKa = 11.84 RR390 pKa = 11.84 MLTTAVTKK398 pKa = 10.63 VATGASDD405 pKa = 3.65 YY406 pKa = 11.23 SVPQHH411 pKa = 5.47 TAGVLTGLNSTDD423 pKa = 3.81 GNLGSQKK430 pKa = 10.48 LPTSIQQDD438 pKa = 3.85 QNDD441 pKa = 4.07 DD442 pKa = 3.37 TAMLNFMRR450 pKa = 11.84 AVAQGMKK457 pKa = 7.77 EE458 pKa = 4.31 TPIQAPPTPGFGSQQAADD476 pKa = 4.58 DD477 pKa = 4.62 DD478 pKa = 4.77 DD479 pKa = 5.88 SRR481 pKa = 11.84 DD482 pKa = 3.69 QADD485 pKa = 2.95 SWGLL489 pKa = 3.37
Molecular weight: 53.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.029
IPC2_protein 5.092
IPC_protein 5.029
Toseland 4.902
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.143
Wikipedia 4.889
Rodwell 4.889
Grimsley 4.813
Solomon 5.003
Lehninger 4.965
Nozaki 5.118
DTASelect 5.308
Thurlkill 4.902
EMBOSS 4.914
Sillero 5.169
Patrickios 4.139
IPC_peptide 5.003
IPC2_peptide 5.169
IPC2.peptide.svr19 5.149
Protein with the highest isoelectric point:
>tr|C0J7I3|C0J7I3_9MONO Fusion glycoprotein F0 OS=Avian orthoavulavirus 9 OX=2560326 GN=F PE=2 SV=1
MM1 pKa = 7.88 DD2 pKa = 5.6 ASRR5 pKa = 11.84 MISLYY10 pKa = 10.66 VDD12 pKa = 3.63 PTSSSSSILAFPIVMEE28 pKa = 4.05 ATGDD32 pKa = 3.46 GRR34 pKa = 11.84 KK35 pKa = 9.5 QISPQYY41 pKa = 9.53 RR42 pKa = 11.84 IQRR45 pKa = 11.84 LDD47 pKa = 2.93 HH48 pKa = 6.58 WSDD51 pKa = 3.06 SSRR54 pKa = 11.84 DD55 pKa = 3.45 AVFITTYY62 pKa = 10.87 GFIFGYY68 pKa = 9.09 PKK70 pKa = 10.46 SRR72 pKa = 11.84 ADD74 pKa = 3.37 RR75 pKa = 11.84 GQLNEE80 pKa = 4.35 EE81 pKa = 3.94 IRR83 pKa = 11.84 PVLLSAATLCLGSVANTGDD102 pKa = 3.17 QVAIARR108 pKa = 11.84 ACLSLQISCKK118 pKa = 10.16 KK119 pKa = 10.37 SATSEE124 pKa = 3.84 EE125 pKa = 4.21 KK126 pKa = 10.15 MIFAITQAPQILQSCRR142 pKa = 11.84 AVSQKK147 pKa = 10.03 FVSVGSNKK155 pKa = 9.51 CVKK158 pKa = 10.28 APEE161 pKa = 4.73 RR162 pKa = 11.84 IEE164 pKa = 5.48 GGQQYY169 pKa = 10.46 DD170 pKa = 3.73 YY171 pKa = 11.2 KK172 pKa = 11.45 VNFVSLTIVPKK183 pKa = 10.5 DD184 pKa = 3.4 DD185 pKa = 3.65 VYY187 pKa = 11.07 RR188 pKa = 11.84 VPKK191 pKa = 9.9 PVLSVSSPTLFRR203 pKa = 11.84 LALSVNIAIDD213 pKa = 3.54 INADD217 pKa = 3.39 NPLSKK222 pKa = 9.61 TLIKK226 pKa = 10.09 TEE228 pKa = 4.23 SGFEE232 pKa = 4.01 ANLFLHH238 pKa = 6.64 VGILSNIDD246 pKa = 3.15 KK247 pKa = 10.36 RR248 pKa = 11.84 GKK250 pKa = 10.08 KK251 pKa = 7.57 VTFEE255 pKa = 4.02 KK256 pKa = 10.79 LEE258 pKa = 4.02 KK259 pKa = 10.12 KK260 pKa = 9.21 IRR262 pKa = 11.84 RR263 pKa = 11.84 MEE265 pKa = 4.0 LTAGLSDD272 pKa = 3.49 MFGPSIILKK281 pKa = 10.42 AKK283 pKa = 10.0 GPRR286 pKa = 11.84 TKK288 pKa = 10.8 LMSAFFSNTGTACYY302 pKa = 9.41 PIAQASPPVSKK313 pKa = 10.63 ILWSQSGHH321 pKa = 4.56 LQEE324 pKa = 5.18 VKK326 pKa = 10.48 ILVQSGTSKK335 pKa = 10.41 MIALTADD342 pKa = 3.33 QEE344 pKa = 4.34 ITTTKK349 pKa = 10.36 LDD351 pKa = 3.38 QHH353 pKa = 7.05 AKK355 pKa = 8.88 IQSFNPFKK363 pKa = 10.82 KK364 pKa = 10.52
Molecular weight: 39.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.935
IPC2_protein 8.829
IPC_protein 8.712
Toseland 9.648
ProMoST 9.341
Dawson 9.838
Bjellqvist 9.531
Wikipedia 9.97
Rodwell 10.335
Grimsley 9.882
Solomon 9.867
Lehninger 9.838
Nozaki 9.75
DTASelect 9.472
Thurlkill 9.706
EMBOSS 10.043
Sillero 9.794
Patrickios 9.531
IPC_peptide 9.867
IPC2_peptide 8.112
IPC2.peptide.svr19 7.8
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4612
364
2210
768.7
85.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.088 ± 0.861
1.93 ± 0.264
5.16 ± 0.424
4.987 ± 0.408
3.404 ± 0.447
5.551 ± 0.276
2.103 ± 0.432
6.83 ± 0.718
4.597 ± 0.443
11.058 ± 1.119
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.348
5.052 ± 0.354
4.271 ± 0.503
4.618 ± 0.509
5.421 ± 0.358
8.283 ± 0.838
6.375 ± 0.526
5.941 ± 0.633
0.867 ± 0.185
3.057 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here