Palaemonetes kadiakensis Mississippi grass shrimp associated circular virus
Average proteome isoelectric point is 9.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RL61|A0A0K1RL61_9CIRC Putative capsid protein OS=Palaemonetes kadiakensis Mississippi grass shrimp associated circular virus OX=1692258 PE=4 SV=1
MM1 pKa = 7.98 RR2 pKa = 11.84 EE3 pKa = 3.8 KK4 pKa = 10.72 EE5 pKa = 4.35 RR6 pKa = 11.84 NNTAAPHH13 pKa = 5.97 EE14 pKa = 4.55 PVSRR18 pKa = 11.84 DD19 pKa = 3.15 PGSIRR24 pKa = 11.84 RR25 pKa = 11.84 TKK27 pKa = 10.24 SIKK30 pKa = 9.74 RR31 pKa = 11.84 DD32 pKa = 3.39 AVSLSRR38 pKa = 11.84 RR39 pKa = 11.84 VARR42 pKa = 11.84 WWLLTIPEE50 pKa = 4.99 DD51 pKa = 4.14 DD52 pKa = 3.9 WNPPADD58 pKa = 3.88 LPAGVAHH65 pKa = 7.39 LKK67 pKa = 10.39 GQLEE71 pKa = 4.42 SGAEE75 pKa = 3.47 GGYY78 pKa = 9.22 RR79 pKa = 11.84 HH80 pKa = 5.73 WQVMVSFTTQVRR92 pKa = 11.84 LPAVKK97 pKa = 10.34 ALFSRR102 pKa = 11.84 TAHH105 pKa = 6.41 CEE107 pKa = 3.64 PSRR110 pKa = 11.84 SDD112 pKa = 4.4 AVDD115 pKa = 3.28 KK116 pKa = 10.42 YY117 pKa = 9.94 VWKK120 pKa = 10.36 EE121 pKa = 3.74 DD122 pKa = 3.21 TRR124 pKa = 11.84 VPGTQFEE131 pKa = 4.61 LGRR134 pKa = 11.84 APVKK138 pKa = 10.76 LNSKK142 pKa = 9.45 PDD144 pKa = 3.08 WDD146 pKa = 5.42 AIWDD150 pKa = 3.68 AAKK153 pKa = 10.52 AGNIEE158 pKa = 5.09 AIPAGIRR165 pKa = 11.84 VRR167 pKa = 11.84 SYY169 pKa = 11.27 ANLRR173 pKa = 11.84 RR174 pKa = 11.84 IRR176 pKa = 11.84 SDD178 pKa = 3.06 HH179 pKa = 6.49 AVALPVEE186 pKa = 4.77 RR187 pKa = 11.84 SCRR190 pKa = 11.84 VFVGPTGTGKK200 pKa = 8.31 SHH202 pKa = 6.92 RR203 pKa = 11.84 AWSEE207 pKa = 3.55 AGMDD211 pKa = 4.88 AYY213 pKa = 10.12 PKK215 pKa = 10.5 DD216 pKa = 3.81 PRR218 pKa = 11.84 TKK220 pKa = 9.95 FWDD223 pKa = 4.42 GYY225 pKa = 10.21 RR226 pKa = 11.84 DD227 pKa = 3.63 QLHH230 pKa = 5.45 VVIDD234 pKa = 3.87 EE235 pKa = 4.08 FRR237 pKa = 11.84 GGIDD241 pKa = 2.99 IAHH244 pKa = 7.88 LLRR247 pKa = 11.84 WLDD250 pKa = 3.59 RR251 pKa = 11.84 YY252 pKa = 9.34 PVNVEE257 pKa = 3.52 IKK259 pKa = 9.72 GASMPLAAKK268 pKa = 9.22 GIWITSNLAPNLWYY282 pKa = 8.39 PAPQHH287 pKa = 5.76 TATLYY292 pKa = 10.89 HH293 pKa = 6.46 PGIRR297 pKa = 11.84 YY298 pKa = 7.98 WSKK301 pKa = 10.82 CCAEE305 pKa = 4.11
Molecular weight: 34.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.945
IPC2_protein 8.902
IPC_protein 8.902
Toseland 9.56
ProMoST 9.341
Dawson 9.823
Bjellqvist 9.575
Wikipedia 10.014
Rodwell 10.072
Grimsley 9.897
Solomon 9.867
Lehninger 9.838
Nozaki 9.677
DTASelect 9.531
Thurlkill 9.677
EMBOSS 9.984
Sillero 9.78
Patrickios 5.423
IPC_peptide 9.867
IPC2_peptide 8.287
IPC2.peptide.svr19 7.924
Protein with the highest isoelectric point:
>tr|A0A0K1RL61|A0A0K1RL61_9CIRC Putative capsid protein OS=Palaemonetes kadiakensis Mississippi grass shrimp associated circular virus OX=1692258 PE=4 SV=1
MM1 pKa = 7.94 PGTLVRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 SRR11 pKa = 11.84 SVGWARR17 pKa = 11.84 RR18 pKa = 11.84 AGAAVSVARR27 pKa = 11.84 GVWRR31 pKa = 11.84 GVKK34 pKa = 9.54 RR35 pKa = 11.84 VRR37 pKa = 11.84 SAISRR42 pKa = 11.84 GRR44 pKa = 11.84 SLARR48 pKa = 11.84 STSRR52 pKa = 11.84 TRR54 pKa = 11.84 SKK56 pKa = 10.83 SRR58 pKa = 11.84 PKK60 pKa = 10.63 SSTRR64 pKa = 11.84 PSSAAPMSVDD74 pKa = 3.53 PQSISHH80 pKa = 6.48 GSVSLRR86 pKa = 11.84 LASPVKK92 pKa = 10.4 GMKK95 pKa = 10.13 SLGRR99 pKa = 11.84 WVYY102 pKa = 7.9 TQQNNGRR109 pKa = 11.84 IVAGLGLQGAGVLLGHH125 pKa = 5.86 NTISQLVVDD134 pKa = 4.18 TAVPNGTQFKK144 pKa = 10.86 DD145 pKa = 3.7 DD146 pKa = 3.79 VFAMNPYY153 pKa = 9.09 QANTGSGIFGSILAPSQDD171 pKa = 3.49 RR172 pKa = 11.84 IMCRR176 pKa = 11.84 SVRR179 pKa = 11.84 TKK181 pKa = 10.86 FMMANTSNAPCVVVLYY197 pKa = 9.7 WVLSKK202 pKa = 10.57 KK203 pKa = 10.11 AHH205 pKa = 5.84 QGNPFDD211 pKa = 3.7 RR212 pKa = 11.84 WTSFLSEE219 pKa = 4.08 TALGQSTATQPNQSDD234 pKa = 3.87 SSTGPGTTGVPTFAVYY250 pKa = 9.76 GQEE253 pKa = 4.0 PTTVRR258 pKa = 11.84 SWRR261 pKa = 11.84 NTFKK265 pKa = 11.12 LLRR268 pKa = 11.84 RR269 pKa = 11.84 KK270 pKa = 9.15 EE271 pKa = 3.92 YY272 pKa = 10.48 HH273 pKa = 5.7 MSFGAVQCINYY284 pKa = 9.55 SVAVNKK290 pKa = 9.86 MFDD293 pKa = 3.38 KK294 pKa = 10.91 TVATQHH300 pKa = 5.47 NTLTSTGYY308 pKa = 9.86 PGGTVWLCAVVRR320 pKa = 11.84 GTRR323 pKa = 11.84 DD324 pKa = 3.44 LVPDD328 pKa = 4.07 CWW330 pKa = 3.7
Molecular weight: 35.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.224
IPC2_protein 10.072
IPC_protein 11.242
Toseland 11.359
ProMoST 11.711
Dawson 11.389
Bjellqvist 11.301
Wikipedia 11.784
Rodwell 11.33
Grimsley 11.433
Solomon 11.784
Lehninger 11.696
Nozaki 11.345
DTASelect 11.301
Thurlkill 11.359
EMBOSS 11.828
Sillero 11.359
Patrickios 11.038
IPC_peptide 11.784
IPC2_peptide 10.73
IPC2.peptide.svr19 9.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
635
305
330
317.5
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.291 ± 1.111
1.417 ± 0.077
4.567 ± 1.208
2.835 ± 1.514
2.52 ± 0.401
7.874 ± 0.957
2.362 ± 0.666
3.622 ± 0.942
4.567 ± 0.493
6.299 ± 0.188
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.047 ± 0.535
3.622 ± 0.488
6.299 ± 0.902
3.307 ± 0.974
9.291 ± 0.158
8.976 ± 1.996
6.772 ± 1.585
8.504 ± 1.176
3.307 ± 0.694
2.52 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here