Streptomyces tateyamensis
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V4N0G0|A0A2V4N0G0_9ACTN Cell surface protein OS=Streptomyces tateyamensis OX=565073 GN=C7C46_19810 PE=4 SV=1
MM1 pKa = 7.43 SVTSEE6 pKa = 4.02 AGASEE11 pKa = 3.94 QLEE14 pKa = 4.19 VWIDD18 pKa = 3.38 QDD20 pKa = 3.99 LCTGDD25 pKa = 5.01 GICAQYY31 pKa = 10.9 APEE34 pKa = 4.23 VFEE37 pKa = 5.9 LDD39 pKa = 3.05 IDD41 pKa = 3.65 GLAYY45 pKa = 10.7 VKK47 pKa = 10.78 GADD50 pKa = 3.46 EE51 pKa = 5.08 EE52 pKa = 4.98 LRR54 pKa = 11.84 QSPGEE59 pKa = 4.11 TVPVPLTLLQDD70 pKa = 3.73 VVDD73 pKa = 4.58 SVRR76 pKa = 11.84 DD77 pKa = 3.73 CPGDD81 pKa = 4.09 CIHH84 pKa = 6.54 VRR86 pKa = 11.84 RR87 pKa = 11.84 ASDD90 pKa = 3.36 GVEE93 pKa = 3.83 VHH95 pKa = 6.92 GPDD98 pKa = 4.03 ADD100 pKa = 3.47
Molecular weight: 10.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.77
IPC_protein 3.745
Toseland 3.528
ProMoST 3.859
Dawson 3.732
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.859
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A2V4P9W5|A0A2V4P9W5_9ACTN Carbonic anhydrase OS=Streptomyces tateyamensis OX=565073 GN=C7C46_03240 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.45 GRR40 pKa = 11.84 ASLSAA45 pKa = 3.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6427
0
6427
2091099
24
6666
325.4
34.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.481 ± 0.047
0.816 ± 0.01
5.448 ± 0.022
5.404 ± 0.036
2.66 ± 0.018
9.444 ± 0.035
2.263 ± 0.013
2.843 ± 0.022
1.833 ± 0.023
11.074 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.518 ± 0.011
1.851 ± 0.024
6.321 ± 0.027
3.394 ± 0.019
7.71 ± 0.043
4.974 ± 0.028
6.125 ± 0.034
8.236 ± 0.027
1.537 ± 0.012
2.067 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here