Dermacoccus sp. 147Ba
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2846 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q4BZC3|A0A4Q4BZC3_9MICO Probable primosomal protein N' OS=Dermacoccus sp. 147Ba OX=2510111 GN=priA PE=3 SV=1
MM1 pKa = 7.99 SEE3 pKa = 4.03 LQYY6 pKa = 10.82 PADD9 pKa = 3.43 LRR11 pKa = 11.84 YY12 pKa = 8.76 TEE14 pKa = 4.03 DD15 pKa = 3.95 HH16 pKa = 6.54 EE17 pKa = 4.53 WVADD21 pKa = 3.51 KK22 pKa = 11.57 GEE24 pKa = 4.13 GVVRR28 pKa = 11.84 VGITAYY34 pKa = 10.48 AQDD37 pKa = 3.56 ALGDD41 pKa = 3.79 VVYY44 pKa = 11.16 VSLPSVGDD52 pKa = 3.57 SVTAGEE58 pKa = 4.36 SCGEE62 pKa = 4.14 VEE64 pKa = 4.43 STKK67 pKa = 10.7 SVSDD71 pKa = 4.28 LYY73 pKa = 11.5 SPLDD77 pKa = 3.72 GEE79 pKa = 4.78 VVAVNDD85 pKa = 4.31 TLDD88 pKa = 3.97 AEE90 pKa = 4.79 PGTVNSDD97 pKa = 3.51 PYY99 pKa = 10.42 GSGWMFEE106 pKa = 4.28 IRR108 pKa = 11.84 PSDD111 pKa = 3.28 ASAIDD116 pKa = 3.69 GLMDD120 pKa = 4.72 ADD122 pKa = 4.96 AYY124 pKa = 9.95 TGSLDD129 pKa = 3.3
Molecular weight: 13.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.81
IPC2_protein 3.541
IPC_protein 3.528
Toseland 3.312
ProMoST 3.668
Dawson 3.541
Bjellqvist 3.732
Wikipedia 3.49
Rodwell 3.363
Grimsley 3.223
Solomon 3.516
Lehninger 3.465
Nozaki 3.656
DTASelect 3.884
Thurlkill 3.389
EMBOSS 3.503
Sillero 3.656
Patrickios 0.54
IPC_peptide 3.503
IPC2_peptide 3.63
IPC2.peptide.svr19 3.677
Protein with the highest isoelectric point:
>tr|A0A4V1VGJ2|A0A4V1VGJ2_9MICO Glutamine synthetase OS=Dermacoccus sp. 147Ba OX=2510111 GN=EVU97_11925 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.46 GRR40 pKa = 11.84 SKK42 pKa = 11.12 LSAA45 pKa = 3.74
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2846
0
2846
933200
28
2216
327.9
35.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.77 ± 0.058
0.715 ± 0.012
6.367 ± 0.04
6.109 ± 0.048
2.96 ± 0.026
8.918 ± 0.043
2.215 ± 0.023
3.73 ± 0.032
2.745 ± 0.041
9.667 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.083 ± 0.018
2.133 ± 0.02
5.217 ± 0.032
2.724 ± 0.022
7.362 ± 0.053
5.765 ± 0.034
6.094 ± 0.034
8.998 ± 0.046
1.494 ± 0.018
1.933 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here