Enterobacteria phage YYZ-2008
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6DZ68|B6DZ68_9CAUD N protein OS=Enterobacteria phage YYZ-2008 OX=564886 PE=4 SV=1
MM1 pKa = 7.62 EE2 pKa = 4.36 FHH4 pKa = 7.21 EE5 pKa = 5.34 SAICDD10 pKa = 3.52 FRR12 pKa = 11.84 ANANSVKK19 pKa = 9.25 PQPIAVLFKK28 pKa = 10.94 TMGAWAVLCFAADD41 pKa = 3.76 DD42 pKa = 4.28 TDD44 pKa = 3.9 DD45 pKa = 3.38 TDD47 pKa = 3.53 ARR49 pKa = 11.84 MAIGQEE55 pKa = 4.02 MEE57 pKa = 3.99 MDD59 pKa = 3.55 PTNDD63 pKa = 2.86 EE64 pKa = 4.58 FIIYY68 pKa = 7.71 GAPSNYY74 pKa = 10.43 LLDD77 pKa = 3.47 TCNIYY82 pKa = 10.99 NKK84 pKa = 10.37 AAA86 pKa = 3.64
Molecular weight: 9.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 4.012
IPC_protein 3.935
Toseland 3.732
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.77
Grimsley 3.643
Solomon 3.91
Lehninger 3.872
Nozaki 4.062
DTASelect 4.279
Thurlkill 3.795
EMBOSS 3.884
Sillero 4.062
Patrickios 1.939
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|B6DZ96|B6DZ96_9CAUD Putative DNAse OS=Enterobacteria phage YYZ-2008 OX=564886 PE=4 SV=1
MM1 pKa = 7.39 SSKK4 pKa = 10.2 NRR6 pKa = 11.84 PRR8 pKa = 11.84 RR9 pKa = 11.84 TTTRR13 pKa = 11.84 NIRR16 pKa = 11.84 FPNQMIEE23 pKa = 4.22 QINIALDD30 pKa = 3.51 QKK32 pKa = 11.1 GSEE35 pKa = 4.26 NFSAWVIEE43 pKa = 4.17 SCRR46 pKa = 11.84 RR47 pKa = 11.84 EE48 pKa = 3.64 LAADD52 pKa = 3.66 IKK54 pKa = 10.46 YY55 pKa = 10.59 ARR57 pKa = 11.84 QLTIKK62 pKa = 10.78 KK63 pKa = 10.17 NDD65 pKa = 3.18 TQYY68 pKa = 11.67 ALRR71 pKa = 11.84 WLFII75 pKa = 4.33
Molecular weight: 8.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.56
IPC_protein 10.116
Toseland 10.584
ProMoST 10.204
Dawson 10.687
Bjellqvist 10.35
Wikipedia 10.862
Rodwell 10.979
Grimsley 10.73
Solomon 10.774
Lehninger 10.745
Nozaki 10.555
DTASelect 10.35
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.599
Patrickios 10.76
IPC_peptide 10.774
IPC2_peptide 9.209
IPC2.peptide.svr19 8.65
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
15830
44
1159
211.1
23.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.431 ± 0.501
1.51 ± 0.163
5.515 ± 0.259
6.431 ± 0.292
3.316 ± 0.154
7.239 ± 0.256
1.92 ± 0.158
5.439 ± 0.242
5.199 ± 0.31
8.168 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.931 ± 0.167
4.239 ± 0.202
4.163 ± 0.278
4.39 ± 0.266
6.759 ± 0.312
6.702 ± 0.274
5.875 ± 0.353
6.519 ± 0.249
1.567 ± 0.127
2.634 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here