Acute bee paralysis virus (strain Rothamsted) (ABPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Picornavirales; Dicistroviridae; Aparavirus; Acute bee paralysis virus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9DSN9|POLN_ABPVR Replicase polyprotein OS=Acute bee paralysis virus (strain Rothamsted) OX=1217067 GN=ORF1 PE=4 SV=1
AA1 pKa = 7.58DD2 pKa = 3.66QEE4 pKa = 4.87TNTSNVHH11 pKa = 4.6NTQLASTSEE20 pKa = 4.2EE21 pKa = 3.77NSVEE25 pKa = 4.1TEE27 pKa = 4.13QITTFHH33 pKa = 7.12DD34 pKa = 3.62VEE36 pKa = 4.49TPNRR40 pKa = 11.84INTPMAQDD48 pKa = 3.47TSSARR53 pKa = 11.84SMDD56 pKa = 3.58DD57 pKa = 2.87THH59 pKa = 9.33SIIQFLQRR67 pKa = 11.84PVLIDD72 pKa = 4.19HH73 pKa = 7.02IEE75 pKa = 4.28VIAGSTADD83 pKa = 4.53DD84 pKa = 3.66NKK86 pKa = 10.59PLNRR90 pKa = 11.84YY91 pKa = 7.77VLNRR95 pKa = 11.84QNPQPFVKK103 pKa = 9.84SWTLPSVVLSAGGKK117 pKa = 7.0GQKK120 pKa = 9.3LANFKK125 pKa = 9.91YY126 pKa = 10.17LRR128 pKa = 11.84CDD130 pKa = 3.36VKK132 pKa = 11.43VKK134 pKa = 10.41IVLNANPFIAGRR146 pKa = 11.84LYY148 pKa = 10.84LAYY151 pKa = 10.2SPYY154 pKa = 10.57DD155 pKa = 3.56DD156 pKa = 4.87RR157 pKa = 11.84VDD159 pKa = 3.58PARR162 pKa = 11.84SILNTSRR169 pKa = 11.84AGVTGYY175 pKa = 9.87PGIEE179 pKa = 4.05IDD181 pKa = 3.88FQLDD185 pKa = 3.19NSVEE189 pKa = 3.97MTIPYY194 pKa = 10.63ASFQEE199 pKa = 4.96AYY201 pKa = 10.49DD202 pKa = 4.0LVTGTEE208 pKa = 4.44DD209 pKa = 3.75FVKK212 pKa = 10.63LYY214 pKa = 10.59LFTITPILSPTSTSASSKK232 pKa = 10.28VDD234 pKa = 3.17LSVYY238 pKa = 8.96MWLDD242 pKa = 3.81NISLVIPTYY251 pKa = 10.34RR252 pKa = 11.84VNTSIVPNVGTVVQTVQNMTTRR274 pKa = 11.84DD275 pKa = 3.26SEE277 pKa = 4.73TIRR280 pKa = 11.84KK281 pKa = 9.68AMVALRR287 pKa = 11.84KK288 pKa = 9.54NNKK291 pKa = 7.69STYY294 pKa = 10.09DD295 pKa = 3.73YY296 pKa = 10.72IVQALSSAVPEE307 pKa = 4.37VKK309 pKa = 10.45NVTMQINSKK318 pKa = 10.32KK319 pKa = 10.14NNSNKK324 pKa = 9.05MATPVKK330 pKa = 9.95EE331 pKa = 3.97KK332 pKa = 10.08TKK334 pKa = 10.62NIPKK338 pKa = 10.15PKK340 pKa = 9.57TEE342 pKa = 4.07NPKK345 pKa = 10.04IGPISEE351 pKa = 4.47LATGVNKK358 pKa = 9.87VANGIEE364 pKa = 4.39RR365 pKa = 11.84IPVIGEE371 pKa = 3.52MAKK374 pKa = 10.32PVTSTIKK381 pKa = 9.9WVADD385 pKa = 3.9KK386 pKa = 10.36IGSVAAIFGWSKK398 pKa = 10.33PRR400 pKa = 11.84NLEE403 pKa = 4.46QVNLYY408 pKa = 10.74QNVPGWGYY416 pKa = 11.16SLYY419 pKa = 10.75KK420 pKa = 10.82GIDD423 pKa = 3.27NSVPLAFDD431 pKa = 4.22PNNEE435 pKa = 4.23LGDD438 pKa = 3.89LRR440 pKa = 11.84DD441 pKa = 3.81VFPSGVDD448 pKa = 3.44EE449 pKa = 4.0MAIGYY454 pKa = 9.03VCGNPAVKK462 pKa = 10.1HH463 pKa = 4.5VLSWNTTDD471 pKa = 5.17KK472 pKa = 11.09VQAPISNGDD481 pKa = 3.2DD482 pKa = 3.08WGGVIPVGMPCYY494 pKa = 10.43SKK496 pKa = 10.59IIRR499 pKa = 11.84TTEE502 pKa = 3.48NDD504 pKa = 3.19TTRR507 pKa = 11.84TNTEE511 pKa = 3.43IMDD514 pKa = 4.76PAPCEE519 pKa = 4.01YY520 pKa = 9.35VCNMFSYY527 pKa = 9.67WRR529 pKa = 11.84ATMCYY534 pKa = 9.5RR535 pKa = 11.84IAIVKK540 pKa = 7.96TAFHH544 pKa = 6.72TGRR547 pKa = 11.84LGIFFGPGKK556 pKa = 10.39IPITTTKK563 pKa = 11.15DD564 pKa = 3.31NISPDD569 pKa = 3.41LTQLDD574 pKa = 4.98GIKK577 pKa = 10.57APSDD581 pKa = 3.43NNYY584 pKa = 10.67KK585 pKa = 10.78YY586 pKa = 10.65ILDD589 pKa = 3.91LTNDD593 pKa = 3.53TEE595 pKa = 4.03ITIRR599 pKa = 11.84VPFVSNKK606 pKa = 8.42MFMKK610 pKa = 9.78STGIYY615 pKa = 10.01GGNSEE620 pKa = 4.6NNWDD624 pKa = 3.7FSEE627 pKa = 4.51SFTGFLCIRR636 pKa = 11.84PITKK640 pKa = 9.49FMCPEE645 pKa = 4.03TVSNNVSIVVWKK657 pKa = 8.83WAEE660 pKa = 3.89DD661 pKa = 3.77VVVVEE666 pKa = 5.19PKK668 pKa = 10.22PLLSGPTQVFQPPVTSADD686 pKa = 3.84SINTIDD692 pKa = 5.49ASMQINLANKK702 pKa = 9.37ADD704 pKa = 3.96EE705 pKa = 4.25NVVTFFDD712 pKa = 3.84SDD714 pKa = 3.73DD715 pKa = 3.9AEE717 pKa = 4.22EE718 pKa = 4.98RR719 pKa = 11.84NMEE722 pKa = 4.4ALLKK726 pKa = 10.88GSGEE730 pKa = 4.09QIMNLRR736 pKa = 11.84SLLRR740 pKa = 11.84TFRR743 pKa = 11.84TISEE747 pKa = 4.12NWNLPPNTKK756 pKa = 8.59TAITDD761 pKa = 3.7LTDD764 pKa = 3.24VADD767 pKa = 3.96KK768 pKa = 10.84EE769 pKa = 4.51GRR771 pKa = 11.84DD772 pKa = 3.59YY773 pKa = 10.9MSYY776 pKa = 10.97LSYY779 pKa = 10.49IYY781 pKa = 10.21RR782 pKa = 11.84FYY784 pKa = 11.14RR785 pKa = 11.84GGRR788 pKa = 11.84RR789 pKa = 11.84YY790 pKa = 10.78KK791 pKa = 10.66FFNTTALKK799 pKa = 10.36QSQTCYY805 pKa = 10.22VRR807 pKa = 11.84SFLIPRR813 pKa = 11.84YY814 pKa = 8.55YY815 pKa = 10.36TADD818 pKa = 3.24NTNNDD823 pKa = 3.91GPSHH827 pKa = 5.53ITYY830 pKa = 9.6PVLNPVHH837 pKa = 6.08EE838 pKa = 4.49VEE840 pKa = 4.31VPYY843 pKa = 10.61YY844 pKa = 9.33CQYY847 pKa = 11.13RR848 pKa = 11.84KK849 pKa = 10.39LPVASTTDD857 pKa = 2.99KK858 pKa = 11.37GYY860 pKa = 10.53DD861 pKa = 3.37ASLMYY866 pKa = 10.06YY867 pKa = 10.68SNVGTNQIVARR878 pKa = 11.84AGNDD882 pKa = 3.1DD883 pKa = 4.41FTFGWLIGTPQTQGITRR900 pKa = 11.84TEE902 pKa = 4.09TKK904 pKa = 10.61

Molecular weight: 100.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9DSN8|POLS_ABPVR Structural polyprotein OS=Acute bee paralysis virus (strain Rothamsted) OX=1217067 GN=ORF2 PE=1 SV=2
PP1 pKa = 7.43IKK3 pKa = 10.59KK4 pKa = 9.97QILPTYY10 pKa = 9.89IIRR13 pKa = 11.84NSRR16 pKa = 11.84RR17 pKa = 11.84PLKK20 pKa = 9.64KK21 pKa = 8.55TQLKK25 pKa = 10.01RR26 pKa = 11.84NKK28 pKa = 9.93SPPFMMWKK36 pKa = 9.54LQIGSIPPWLKK47 pKa = 9.08TLHH50 pKa = 7.03RR51 pKa = 11.84LGAWMIRR58 pKa = 11.84TVLFSFYY65 pKa = 10.34NAPYY69 pKa = 10.62SLTTLRR75 pKa = 11.84SLLDD79 pKa = 3.56QQQMITNPSIDD90 pKa = 3.22MCC92 pKa = 5.56

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2902

92

1906

967.3

110.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.583 ± 0.555

1.654 ± 0.297

6.168 ± 0.612

6.203 ± 1.412

4.273 ± 0.418

4.962 ± 0.427

1.413 ± 0.272

7.512 ± 0.379

7.547 ± 0.951

7.788 ± 1.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.432

6.444 ± 0.589

4.376 ± 1.333

3.377 ± 0.461

4.342 ± 0.481

6.685 ± 0.408

6.513 ± 1.395

7.478 ± 0.943

1.516 ± 0.257

3.928 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski